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Bulletin of Botanical Research ›› 2008, Vol. 28 ›› Issue (5): 603-607.doi: 10.7525/j.issn.1673-5102.2008.05.021

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Physiological and Biochemical Characteristics of Salt-tolerance Callus of Flax

GE Chun-Hui;JI Qiao-Ling*;WANG Xue-Hua;JIA Hong-Li;ZHU Guo-Li;ZHANG Pei-Hong   

  1. (College of life science and technology,Xinjiang university,Urumqi 830046)
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-09-20 Published:2008-09-20
  • Contact: JI Qiao-Ling
  • Supported by:

Abstract: The salt-tolerance callus of flax were used as experimental materials and common callus of flax were used as controls. After being cultured individually on the mediums containing 0~250 mmol·L-1 NaC1 for 20 d, the water content, content of MDA, the POD activity, the SOD activity, esterase isozyme zymogram, POD isozyme zymogram and SOD isozyme zymogram were compared individually. The results indicated that there were obvious differences in many aspects between the callus of salt-tolerance groups and the callus of control groups of flax. The water contents, the contents of MDA and the POD activities in the former were all higher than those in the latter as well as esterase activities. Esterase isozyme zymograms of callus of salttolerance groups were different from those of callus of contrast groups. There was more POD isozyme band in salt-tolerance groups. SOD isozyme bands in salt-tolerance groups were as many as those in control groups, SOD quality in the former was higher and stable. The stody laid a sound base for the further screening of salt-tolerance mutant cell line of flax.

Key words: flax(Shuangya No.5), salt-tolerance callus, content of MDA, POD, SOD activity, esterse, peroxidase and superoxide dismutase isozymes

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