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Bulletin of Botanical Research ›› 2008, Vol. 28 ›› Issue (5): 579-583.doi: 10.7525/j.issn.1673-5102.2008.05.016

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Cloning and Analysis of CCS Gene of Newhall Navel Orange

TAO Neng-Guo;ZHANG Ji-Hong   

  1. (College of Chemical Engineering,Xiangtan University,Xiangtan 411105)
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-09-20 Published:2008-09-20
  • Contact: TAO Neng-Guo
  • Supported by:

Abstract: The mRNA sequence for Capsanthin/Capsorubin synthase (CCS) has been cloned from fruits of Newhall navel orange by RT-PCR. Sequencing results revealed that it was 1 619 bp in length, encoding 503 amino acids. BLAST results showed that the deduced amino acids shared the same amino acid sequence as that of Citrus sinensis, 70% identities with CCS of Daucus carota and Capsicum annuum. In addition to 70% identities with neoxanthin synthase (NSY) of Solanum tuberosum, it showed 50%~70% identities with lycopene cyclase of Lycopersicon esculentum and citrus. Semi-quantitative RT-PCR results showed that expressions of Ccs were down-regulated during fruit ripening of Newhall navel orange, with the highest one in September.

Key words: RT-PCR, citrus, Capsanthin/Capsorubin synthase, gene

CLC Number: