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Bulletin of Botanical Research ›› 2008, Vol. 28 ›› Issue (1): 67-72.doi: 10.7525/j.issn.1673-5102.2008.01.015

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Cloning and Characterization of Beclin1-ike Gene in Eupatorium odoratum L. buds

QI Chang-Qing;LIU Ying;WANG Li;LI Si-Bin;WANG Min;JANG Yang;ZU Yuan-Gang*   

  1. Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-01-20 Published:2008-01-20
  • Contact: ZU Yuan-Gang
  • Supported by:

Abstract: Beclin1-like gene was cloned from three different growth periods flower buds of Eupatorium odoratum by RT-PCR method in this study. The homology between Beclin1-like gene (about 700bp) and Beclin1 gene in tobacco leaves is 95%; Northern blotting result showed that the expression of Beclin1-like gene in the middle stage was higher than those in initial stages and dernier stage; the result of DNA ladder showed Programmed Cell Death (PCD) happened in the process of flower buds. All the results proved that PCD certainly happened with the tapetum degenerating and the pollen coming into being, which indicated the relationship between the aggressive process of E. odoratum and PCD in the process of the reproduction.

Key words: Eupatorium odoratum, buds, Beclin1 gene, Programmed Cell Death (PCD), tapetum

CLC Number: