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Bulletin of Botanical Research ›› 2006, Vol. 26 ›› Issue (6): 703-707.doi: 10.7525/j.issn.1673-5102.2006.06.013

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Studied the difference of genetic feature between tissue cultured and cultivated paddy rice of Jia He Zao Zan via microsatellite(SSR) markers

ZHOU Ke-Fu;ZHANG Kai;RONG Wen-Ting;HUANG Yu-Ming;WANG Hou-Cong   

  1. (The Key Laboratory of Education Ministry for Cell Biology and Tumor Cell Xiamen University,Fujian 361005)
  • Received:1900-01-01 Revised:1900-01-01 Online:2006-11-20 Published:2006-11-20
  • Contact: ZHOU Ke-Fu
  • Supported by:

Abstract: Plant height is one of the important agronomic traits of rice. Overhigh plant easily led to lodge and reduce output. Recently, great advances have been made in the identification, mapping,cloning and action mechanism of plant height genes in rice. This present paper selected the tissue cultured and cultivated paddy rice of Jia He Zao Zan(J.H.Z.Z) as experimental object, which were significant different on the height, the tissue cultured plant was only less than half height of the cultivated plant. In order to compare their differences of genetic and determine whether there are relationship between the dwarf gene and the dwarf character of tissue cultured plant, Polymorphism analysis was conducted between them by 311 pairs of rice SSR primers, designed by Cornell University, the result indicated that 88 pairs of primers had polymorphism between two kinds of materials, the ration in total primers was about 30.3%, the experimental result not only verified 11 multiple groups which regulate the height gene, but also obtained two group regions which control the dwarf character on chromosome 3 and chromosome 9, which have not been reported previously, these results will help to explore and determine the situation of new dwarf gene, and will be of benefit to act genetic manipulation of plant height of in rice breeding. These results also provided the evidence for studying the mechanism of dwarf.

Key words: molecular marker, SSR, dwarf character

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