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Bulletin of Botanical Research ›› 2005, Vol. 25 ›› Issue (4): 441-443.doi: 10.7525/j.issn.1673-5102.2005.04.014

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The study on secondary culturation after the protoplasts of Arabidopsis thaliana broken by ultrasonic

ZU Yuan-Gang, MENG Qing-Huan, GUO Xiao-Rui, SUN Jia-Yin, LIU Hong-Mei   

  1. Key Laboratory of Forest Plant Ecolory, Ministry of Education, Northeast Forestry University, Harbin 150040
  • Received:2005-07-07 Online:2005-12-15 Published:2016-06-14
  • Supported by:
    Supported by the State Key Basic Research Project (G19990160) and the Key Project of Chinese Ministry of Education (104191)

Abstract: After the protoplasts of Arabidopsis thaliana was broken by the ultrasonic and filtrated by the filter membrane,we subcultured the organs such as chloroplast and nucleus.It's observed that some chloroplasts were no fluorescence under the blue excitated light.The result indicated the ultrasonic had certain functions of destroying the chloroplasts.After being cultured for 115 h,the chloroplasts began to gather;and the nuclei obviously increased;also the bright green dots of endoplasmic reticulum appeared.As we all known the nuclei and endoplasmic reticulum are essential cell organs which form the new protoplast,so culturing cell organs may offer us some enlightenments about organs recombination and even for the cells reconstruction.

Key words: Arabidopsis thaliana, protoplast, organ recombination, fluorescent probe