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Bulletin of Botanical Research ›› 2005, Vol. 25 ›› Issue (4): 427-431.doi: 10.7525/j.issn.1673-5102.2005.04.011

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Cloning and sequencing analysis on the cDNA fragment SI800 separated from rye which related with salt tolerance

WANG Zhen-Ying1, PENG Yong-Kang1, ZHENG Jian-Yu2   

  1. 1. Department of Biology, Tianjin Normal University, Tianjin 300074;
    2. Department of Life Science, Nan Kai University, Tianjin 300071
  • Received:2004-12-20 Online:2005-12-15 Published:2016-06-14

Abstract: SI800 (Salt Induced(800 bp), SI800) was a cDNA fragment, which separated from rye leaves by DDRT-PCR.In this study, we purified SI800 by low temperature agarose electrophoresis and linked to pGEM-T vector then transformed to Escherichia coli(JM 109).30 positive clones were obtained.2 positive clones were selected randomly after test by digestion, PCR and sequenced.The result indicated that SI800 was composed of(735 bp).DNA sequence analysis showed(68%) homologue to uxaB gene for altronate oxidoreductase in GenBank.

Key words: rye, salt tolerance, cloning of cDNA fragment, sequencing