Cloning and sequencing analysis on the cDNA fragment SI800 separated from rye which related with salt tolerance

doi:10.7525/j.issn.1673-5102.2005.04.011

Bulletin of Botanical Research ›› 2005, Vol. 25 ›› Issue (4): 427-431.doi: 10.7525/j.issn.1673-5102.2005.04.011

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Cloning and sequencing analysis on the cDNA fragment SI₈₀₀ separated from rye which related with salt tolerance

WANG Zhen-Ying¹, PENG Yong-Kang¹, ZHENG Jian-Yu²

1. Department of Biology, Tianjin Normal University, Tianjin 300074;
2. Department of Life Science, Nan Kai University, Tianjin 300071

Received:2004-12-20 Online:2005-12-15 Published:2016-06-14

Abstract

Abstract: SI₈₀₀ (Salt Induced(800 bp), SI₈₀₀) was a cDNA fragment, which separated from rye leaves by DDRT-PCR.In this study, we purified SI₈₀₀ by low temperature agarose electrophoresis and linked to pGEM-T vector then transformed to Escherichia coli(JM 109).30 positive clones were obtained.2 positive clones were selected randomly after test by digestion, PCR and sequenced.The result indicated that SI₈₀₀ was composed of(735 bp).DNA sequence analysis showed(68%) homologue to uxaB gene for altronate oxidoreductase in GenBank.

Key words: rye, salt tolerance, cloning of cDNA fragment, sequencing

WANG Zhen-Ying, PENG Yong-Kang, ZHENG Jian-Yu. Cloning and sequencing analysis on the cDNA fragment SI₈₀₀ separated from rye which related with salt tolerance[J]. Bulletin of Botanical Research, 2005, 25(4): 427-431.

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Cloning and sequencing analysis on the cDNA fragment SI₈₀₀ separated from rye which related with salt tolerance

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