温度胁迫对白车轴草水浸提液化感作用的影响

doi:10.7525/j.issn.1673-5102.2010.02.020

植物研究 ›› 2010, Vol. 30 ›› Issue (2): 243-247.doi: 10.7525/j.issn.1673-5102.2010.02.020

温度胁迫对白车轴草水浸提液化感作用的影响

何杨艳^1,2;马丹炜^1*;李群¹;王亚男¹;田佳乔¹;成为为¹

1.四川师范大学生命科学学院，成都　610066；2.四川大学华西医院生物治疗国家重点实验室，成都　610041

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-03-20 发布日期:2010-03-20
通讯作者: 马丹炜
基金资助:

Temperature Influences on the Allelopathy Effect of Aqueous Extracts from Trifolium repens L.

HE Yang-Yan;MA Dan-Wei*;LI Qun;WANG Ya-Nan;TIAN Jia-Qiao;CHENG Wei-Wei

1.Life science college，Sichuan normal university，Chengdu　610066；2.West China Hospital,Sichuan University,Chengdu　610041

Received:1900-01-01 Revised:1900-01-01 Online:2010-03-20 Published:2010-03-20
Contact: MA Dan-Wei
Supported by:

摘要/Abstract

摘要： 采用蚕豆根尖细胞微核试验和染色体畸变试验，研究了温度胁迫下白车轴草水浸提液对蚕豆根尖细胞的化感作用。结果表明：不同温度（5℃、25℃和35℃)处理后的白车轴草的水浸提液对蚕豆根尖细胞有丝分裂指数的影响为低浓度促进，高浓度抑制。在白车轴草水浸提液的作用下，蚕豆根尖细胞内出现了多种染色体畸变，如微核、染色体断片、染色体桥、落后染色体等，对微核率和染色体畸变率的诱导效应表现为随水浸提液浓度的增大而增大。温度胁迫后的化感作用明显大于胁迫前，其化感效应表现为低温(5℃)下白车轴草水浸提液>高温(35℃)>常温(25℃)白车轴草水浸提液。推测原因可能是温度胁迫改变和增加了化感物质的释放。

关键词: 白车轴草, 化感作用, 微核试验, 染色体畸变试验

Abstract: The allelopathy effect of aqueous extracts from Trifolium repens L. on root tip cells of Vicia faba under different temperature (5℃,25℃ and 35℃) stresses was investigated by using micronucleus assay and chromosome aberration assay. The results indicated that the mitotic index of V.faba root tip cells treated by low concentration of aqueous extracts increased and it decreased at high concentration, the aqueous extracts of T.repens also caused various types of chromosome aberrations such as micronucleus, chromosome fragment, chromosomal bridge and lagging chromosome,and micronucleus and aberration rates increased along with concentration of extracts. This research showed the allelopathy effects of aqueous extracts from T.repens by different temperature treatments were different, the low temperature group (5℃) was the strongest, high temperature group (35℃) was second, normal temperature（25℃）was the smallest, which was probably due to temperature stress changed and increased the secondary metabolites of T.repens, increased genetic toxicity of the aqueous extracts of this plant.

Key words: Trifolium repens L., Genetic toxicity, micronucleus assay, chromosome aberration assay

中图分类号:

S513
S161.4

何杨艳;马丹炜*;李群;王亚男;田佳乔;成为为. 温度胁迫对白车轴草水浸提液化感作用的影响[J]. 植物研究, 2010, 30(2): 243-247.

HE Yang-Yan;MA Dan-Wei*;LI Qun;WANG Ya-Nan;TIAN Jia-Qiao;CHENG Wei-Wei. Temperature Influences on the Allelopathy Effect of Aqueous Extracts from Trifolium repens L.[J]. Bulletin of Botanical Research, 2010, 30(2): 243-247.

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温度胁迫对白车轴草水浸提液化感作用的影响

Temperature Influences on the Allelopathy Effect of Aqueous Extracts from Trifolium repens L.

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