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植物研究 ›› 2014, Vol. 34 ›› Issue (5): 671-677.doi: 10.7525/j.issn.1673-5102.2014.05.014

• 论文 • 上一篇    下一篇

福建武夷山甜槠不同世代种群遗传多样性的SSR分析

周晓丽1;杨青2;丁晖3;方炎明1*;徐海根3;陈晓2   

  1. 1.南京林业大学森林资源与环境学院,南京 210037;2.武夷山生物研究所,武夷山 354300;3.环境保护部南京环境科学研究所,南京 210044
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2014-09-20 发布日期:2015-03-19
  • 通讯作者: 方炎明
  • 基金资助:
     

SSR Analysis on Genetic Diversity of Different Generations in Castanopsis eyrei from Wuyishan,Fujian Province

ZHOU Xiao-Li;YANG Qing;DING Hui;FANG Yan-Ming*;XU Hai-Gen;CHEN Xiao   

  1. 1.College of Forest Resources and Environment,Nanjing Forest University,Nanjing 210037;2.Wuyishan Institute of Biology,Wuyishan 354300;3.Nanjing Institute of Environmental Sciences,Ministry of Environmental Protection,Nanjing 210044
  • Received:1900-01-01 Revised:1900-01-01 Online:2014-09-20 Published:2015-03-19
  • Contact: FANG Yan-Ming
  • Supported by:
     

摘要: 按胸径将福建武夷山大安源样地的甜槠(Castanopsis eyrei)群体划分为成体、小树、幼苗3个世代,利用SSR分子标记对不同世代的甜槠遗传多样性及遗传分化进行分析,旨在揭示其不同世代间的遗传变异规律,为甜槠资源的保护与利用提供科学依据。14对SSR引物共检测到92个等位基因,平均每位点的等位基因数A=6.571 4,居群的平均有效等位基因数Ae=3.905 4,平均期望杂合度He=0.722 9,表明甜槠群体具有丰富的遗传变异。SSR分析显示3个世代的AeHe、Nei指数(h)、Shannon信息指数(I)均以幼苗最高,小树次之,成体最低,幼苗的遗传多样性指数高于成体及小树,且幼苗中出现最多的稀有等位基因数,表明甜槠种群世代间的遗传多样性呈稳定上升趋势。分子方差分析(AMOVA)表明甜槠群体不同世代内、世代间均存在遗传变异,但遗传变异主要存在于世代内。SSR分析显示,甜槠不同世代间的遗传分化系数Fst=0.074 3,基因流Nm=3.115 4。甜槠不同世代间的遗传相似度以成体与幼苗最小,遗传距离以成体与幼苗间最大。基于甜槠群体SSR的研究结果,认为自然保护区的建立对物种遗传多样性的保护具有重要作用,并提出在遗传多样性保护中应注重保护成体和幼苗中稀有的等位变异。

关键词: 甜槠, 世代, 遗传多样性, 遗传分化, SSR

Abstract: The populations of Castanopsis eyrei collected from Daanyuan plots in Wuyishan city, Fujian province were divided into 3 generations according to the size class, i.e. adults, juveniles, seedlings. A total of 92 alleles were detected with 14 pairs of SSR primers, and a mean of 6.571 4 alleles were detected with per pair primer. The mean effective number of alleles (Ae) and expected heterozygosity (He) at population level were 3.905 4 and 0.722 9, respectively. These data suggested that the genetic diversity of C.eyrei was quite high. SSR analyses showed that the highest effective number of alleles (Ae), expected heterozygosity (He), Nei index, Shannon information index were detected in seedlings, juveniles took the second place and the lowest were detected in adults. The most rare alleles was found in seedlings. It suggested that the trend of genetic diversity of the population of C.eyrei was in ascension. Analysis of molecular variance (AMOVA) showed that the genetic diversity might exist within and among different generations of C.eyrei populations, but mainly exist within generations. SSR analyses showed that gene differentiation coefficient (Fst) among generations of C.eyrei populations was 0.074 3 while the gene flow (Nm) was 3.115 4. The genetic identity between adults and seedlings was the lowest. The genetic distance between adults and seedlings was the highest. Based on the analysis of SSR, establishment of nature reserve and conservation of the rare alleles in seedlings and adults is of great importance.

Key words: Castanopsis eyrei, generation, genetic diversity, genetic differentiation, SSR

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