smGFP最小线性化元件的直接渗透转化与瞬时表达

doi:10.7525/j.issn.1673-5102.2009.02.018

植物研究 ›› 2009, Vol. 29 ›› Issue (2): 230-233.doi: 10.7525/j.issn.1673-5102.2009.02.018

smGFP最小线性化元件的直接渗透转化与瞬时表达

徐凤萍;杨君^*;程云清;刘建凤;安利佳

(大连理工大学生物科学与工程系，大连　116023)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-03-20 发布日期:2009-03-20
通讯作者: 杨君
基金资助:

Direct DNA Transformation of Minimal Linear smGFP Gene Cassette and Its Transient Expression in Onion Epidermal Cell

XU Feng-Ping;YANG Jun*;CHENG Yun-Qing;LIU Jian-Feng;AN Li-Jia

(Department of Bioscience and Biotechnology,Dalian University of Technology,Dalian　116023)

Received:1900-01-01 Revised:1900-01-01 Online:2009-03-20 Published:2009-03-20
Contact: YANG Jun
Supported by:

摘要/Abstract

摘要： 通过转化缓冲溶液调整转化系统的渗透压，实现了含有smGFP基因的最小线性化元件在洋葱表皮细胞中的直接转化和瞬时表达。利用荧光显微镜在细胞质和细胞核中观察到很强的绿色荧光蛋白的表达，表明smGFP线性化元件顺利导入细胞内，并维持了表达元件的完整性。该方法仅通过缓冲溶液的设计实现外源基因的直接转化，无需基因枪、电激、注射等转化动力来源，能够显著减少实验操作对受体材料的破坏性，扩大转化受体的应用范围，同时操作简便快捷，大大降低了实验成本。

关键词: smGFP, 直接转化, 瞬时表达, 线性化元件

Abstract: The minimal linear smGFP gene cassette was constructed and transiently expressed in onion epidermal cell by adjusting the osmotic pressure of the transformation buffer. Strong fluorescence of green fluorescent protein was observed in cytoplasm and nucleus by fluorescence microscope, which proved that smGFP gene cassettes transformed into onion epidermal cell and kept its integrity. Exogenous gene was penetrated into host cell directly without any other force, such as particle bombardment, electransformation and microinjection. Therefore, it induces less damage to the host cells and has a wide range of application. In addition, this method is easily operated and less costly.

Key words: smGFP, direct transformation, transient expression, minimal linear transformation cassette

中图分类号:

Q786

徐凤萍;杨君*;程云清;刘建凤;安利佳. smGFP最小线性化元件的直接渗透转化与瞬时表达[J]. 植物研究, 2009, 29(2): 230-233.

XU Feng-Ping;YANG Jun*;CHENG Yun-Qing;LIU Jian-Feng;AN Li-Jia. Direct DNA Transformation of Minimal Linear smGFP Gene Cassette and Its Transient Expression in Onion Epidermal Cell[J]. Bulletin of Botanical Research, 2009, 29(2): 230-233.

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smGFP最小线性化元件的直接渗透转化与瞬时表达

Direct DNA Transformation of Minimal Linear smGFP Gene Cassette and Its Transient Expression in Onion Epidermal Cell

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