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植物研究 ›› 2014, Vol. 34 ›› Issue (4): 479-484.doi: 10.7525/j.issn.1673-5102.2014.04.009

• 论文 • 上一篇    下一篇

红花荷天然群体的遗传多样性分析

徐斌;朱报著;张方秋;潘文;王裕霞   

  1. 广东省林业科学研究院,广州 510520
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2014-07-20 发布日期:2014-07-20
  • 基金资助:
     

Genetic Diversity Analysis on Rhodoleia championii Wild Populations

XU Bin;ZHU Bao-Zhu;ZHANG Fang-Qiu;PAN Wen;WANG Yu-Xia   

  1. Guangdong Academy of Forestry,Guangzhou 510520
  • Received:1900-01-01 Revised:1900-01-01 Online:2014-07-20 Published:2014-07-20
  • Supported by:
     

摘要: 利用ISSR分子标记技术,对红花荷主要分布区8个天然群体的遗传多样性和遗传结构进行研究。结果表明:红花荷在物种水平上其多态位点百分率(PPB)为94.86%,Shannon表型多样性指数(H0)为0.343 8;在群体水平上PPB为73.07%,H0为0.276 3,说明红花荷群体具有丰富的遗传多样性。AMOVA分子方差分析表明,红花荷遗传变异主要来自群体内,群体内遗传变异占群体的70.29%,群体间占29.71%。Mantel检测表明红花荷群体间的遗传距离与地理距离之间没有明显相关性。

关键词: 红花荷, ISSR, 遗传多样性, 遗传结构

Abstract: The genetic diversities and genetic structures of 8 Rhodoleia championii wild populations were evaluated using ISSR markers. The results showed that R.championii had higher genetic diversity at both species level (percentage of polymorphic bands (PPB)=94.86%, Shannon’s phenotypic diversity index (H0)=0.343 8) and population level (PPB=73.07%, H0=0.267 3). The analysis of molecular variance (AMOVA) showed that genetic differentiation among and within populations was all found to be remarkably high, and 29.71% of genetic variation existed among populations. The Mantel test showed that the geographic and genetic distances were not significantly correlated to each other.

Key words: Rhodoleia championii, ISSR, genetic diversity, genetic structure

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