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植物研究 ›› 2014, Vol. 34 ›› Issue (3): 428-432.doi: 10.7525/j.issn.1673-5102.2014.03.022

• 论文 • 上一篇    

超声辅助提取杜仲多糖及其抗氧化活性

孙曦晓1;刘再枝1;杜新琦1;赵春建1*;王化3;杨磊1;汪贵斌2;祖元刚1   

  1. 1.东北林业大学森林植物生态学教育部重点实验室,哈尔滨 150040;2.南京林业大学森林资源与环境学院,南京 210037;3.黑龙江省科学院自然与生态研究所,哈尔滨 150040
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2014-05-20 发布日期:2014-05-20
  • 通讯作者: 赵春建
  • 基金资助:
     

Ultrasonic-assisted Extraction of Polysaccharide from Eucommia ulmoides and Evaluation of its Antioxidant Activity

SUN Xi-Xiao;LIU Zai-Zhi;DU Xin-Qi;ZHAO Chun-Jian*;WANG Hua;YANG Lei;WANG Gui-Bin;ZU Yuan-Gang   

  1. 1.Key Laboratory of Forest Plant Ecology,Ministry of Education,Northeast Forestry University,Harbin 150040;2.College of Forest Resources and Environment,Nanjing Forestry University,Nanjing 210037;3.Institute of Nature and Ecology,Heilongjiang Academy of Sciences,Harbin 150040
  • Received:1900-01-01 Revised:1900-01-01 Online:2014-05-20 Published:2014-05-20
  • Contact: ZHAO Chun-Jian
  • Supported by:
     

摘要: 以杜仲树皮为原料,采用超声辅助法提取杜仲多糖,考察了预浸泡时间、超声功率、超声时间、超声温度、料液比和提取次数对杜仲多糖得率的影响,最佳提取条件为:预浸泡时间2 h,超声功率250 W,超声时间30 min,超声温度40℃,料液比1∶12 g·mL-1,提取次数2次。在上述提取条件下提取杜仲多糖,多糖得率为1.94%。此外,对杜仲多糖的抗氧化活性进行了分析评价。结果表明,在相同浓度下,杜仲多糖对DPPH自由基的清除能力高于维生素C。然而,杜仲多糖的铁还原能力低于维生素C的铁还原能力,并且与浓度呈正相关。

关键词: 杜仲多糖, DPPH自由基的清除能力, 铁还原能力, 维生素C

Abstract: Ultrasonic-assisted extraction was used for the extraction of polysaccharide from Eucommia ulmoides bark. The effects of the pre-soaking time, the ultrasonic power, the extracting time, the ultrasonic temperature, the ratio of liquid to solid and the extraction times were examined. The optimal conditions for ultrasound extraction were obtained as following: pre-soaking time 2 h, extraction power 250 W, extracting time 30 min, ultrasonic temperature 40℃, liquid to solid ratio 1∶12 g·mL-1, and extraction 2 times. The highest polysaccharide yield (1.94%) was obtained under the optimal extraction condition. In addition, the antioxidant activity of polysaccharide from E.ulmoides was analyzed and evaluated. The results showed that the DPPH free-radical scavenging ability of polysaccharide is much higher than that of vitamin C at the same concentration. While compared with vitamin C, it exhibited a moderate and concentration-dependent ferric-reducing power.

Key words: Eucommia ulmoides polysaccharide, DPPH free-radical scavenging ability, ferric-reducing power, vitamin C

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