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植物研究 ›› 2012, Vol. 32 ›› Issue (6): 765-769.doi: 10.7525/j.issn.1673-5102.2012.06.023

• 论文 • 上一篇    

响应面优化纤维素酶辅助提取木豆叶总黄酮工艺

王秋雪1,2;顾成波1,2*;付丽楠1,2;李旺1,2;袁肖寒3;祖元刚1,2;付玉杰1,2   

  1. 1.东北林业大学森林植物生态学教育部重点实验室,哈尔滨 150040;2.东北林业大学林业生物制剂教育部工程研究中心, 哈尔滨 150040;3.东北农业大学生命科学与生物技术研究中心,哈尔滨 150030
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-11-20 发布日期:2012-11-20
  • 通讯作者: 顾成波
  • 基金资助:
     

Optimization of Cellulase-assisted Extraction Process of Total Flavonoids from Pigeon Pea Leaves Using Response Surface Methodology

WANG Qiu-Xue;GU Cheng-Bo;*;FU Li-Nan;LI Wang;YUAN Xiao-Han;ZU Yuan-Gang;FU Yu-Jie;   

  1. 1.Key Laboratory of Forest Plant Ecology of Northeast Forestry University,Ministry of Education,Harbin 150040;2.Engineering Research Center of Forest Biopreparation,Ministry of Education,Northeast Forestry University,Harbin 150040;3.Life Science and Biotechnique Research Center,Northeast Agricultural University,Harbin 150030
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-11-20 Published:2012-11-20
  • Contact: GU Cheng-Bo
  • Supported by:
     

摘要: 为探讨纤维素酶辅助提取木豆叶总黄酮最佳工艺条件,在单因素试验基础上,以酶用量、酶解温度、酶解时间和酶解pH为影响因子,总黄酮得率为响应值,采用Box-behnken中心组合设计建立4个影响因子与总黄酮得率关系的数学模型,进行响应面法分析。结果表明,纤维素酶辅助提取木豆叶总黄酮的最佳工艺条件为:酶用量为8.65 mg,酶解温度为33.88℃,酶解时间为2.02 h,酶解pH为5.02。在最优条件下总黄酮理论得率为4.86%,实测值为4.88%,拟合得到的模型与实际吻合良好。本研究建立的提取工艺条件稳定可靠,为以后木豆叶总黄酮的应用开发提供实验依据。

关键词: 木豆, 总黄酮, 纤维素酶, 响应面

Abstract: Response surface methodology (RSM) was used to optimize the cellulase-assisted extraction process of total flavonoids (TF) from pigeon pea leaves. The amount of enzyme, enzymatic hydrolysis temperature, time and pH were selected as influencing factors and TF yield as the response value based on single factor trials. The experiment mathematical model was established according to Box-Behnken central composite experiment design and was analyzed by RSM. The results showed the optimal extraction conditions were as follows:enzyme amount 8.65 mg, extraction temperature 33.88℃, extraction time 2.02 h and extraction pH 5.02. The predicted value of TF yield was 4.86% under the optimum conditions, and the experimental value was 4.88%. The optimal extraction conditions were stable and reliable, and would provide experimental basis for the future development of TF in pigeon pea leaves.

Key words: pigeon pea, total flavonoids, cellulase, response surface methodology

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