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植物研究 ›› 2012, Vol. 32 ›› Issue (6): 724-730.doi: 10.7525/j.issn.1673-5102.2012.06.016

• 论文 • 上一篇    下一篇

油菜BnHMGB2基因的克隆及表达分析

张腾国1;王娟1;王圆圆1;王宁1;常燕1;张艳1;陈琼琼1;孙万仓2   

  1. 1.西北师范大学生命科学学院,兰州 730070;2.甘肃农业大学农学院,兰州 730070
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-11-20 发布日期:2012-11-20
  • 基金资助:
     

Cloning and Expression Analysis of BnHMGB2 Gene in Brassica napus

ZHANG Teng-Guo;WANG Juan;WANG Yuan-Yuan;WANG Ning;CHANG Yan;ZHANG Yan;CHEN Qiong-Qiong;SUN Wan-Cang   

  1. 1.School of Life Science,Northwest Normal University,Lanzhou 730070;2.College of Agronomy,Gansu Agricultural University,Lanzhou 730070
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-11-20 Published:2012-11-20
  • Supported by:
     

摘要: 通过RACE技术从陇油6号油菜中克隆得到了一种新的BnHMGB2基因的cDNA,全长823 bp,其中包括438 bp的开放阅读框,131 bp的5′非翻译区(5′UTR),253 bp的3′非翻译区(3′UTR)。与拟南芥AtHMGB2的同源性达到了87.4%,因此命名为BnHMGB2(GenBank登录号:JN807314)。该基因编码145个氨基酸的蛋白质,分子量15.9 KDa,等电点为5.63。实时荧光定量PCR结果表明该基因在油菜根、茎、叶、下胚轴均有表达,根中表达量最高。同时,该基因的表达受低温胁迫的诱导,表明该基因在油菜适应低温胁迫的过程中发挥作用。

关键词: 陇油6号, BnHMGB2基因, 分子克隆, 表达分析

Abstract: A new cDNA BnHMGB2 was isolated from Brassica napus long you6 by RACE. The cloned full-length cDNA was 823 bp, contained a 438 bp ORF, a 5′untranslated region (5-UTR) of 131 bp, and a 3′untranslated region (3′UTR) of 253 bp. The nucleotide sequence of BnHMGB2 was 87.4% identical with the Arabidopsis thaliana AtHMGB2, so it was named BnHMGB2(GenBank accession: JN807314). BnHMGB2 encoded a protein of 145 amino acids with a deduced molecular weight of about 15.9 KDa and with a PI of 5.63. Analysis by real-time RT-PCR indicated that BnHMGB2 was expressed in root, stem, leaf and hypocotyl of B.napus and relatively higher abundance was observed in root tissue. Sequence analysis indicated that the BnMKK2 protein exhibited closest homology to MAPKK which is responded to cold stress, and BnHMGB2 played an important role in defending cold stress.

Key words: longyou 6, BnHMGB2 gene, gene cloning, expression analysis

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