β-环糊精包合红松籽油的制备工艺及生物利用度评价

doi:10.7525/j.issn.1673-5102.2020.02.020

植物研究 ›› 2020, Vol. 40 ›› Issue (2): 314-320.doi: 10.7525/j.issn.1673-5102.2020.02.020

• 研究简报 • 上一篇

β-环糊精包合红松籽油的制备工艺及生物利用度评价

祖述冲

东北林业大学森林植物生态学教育部重点实验室, 哈尔滨 150040

收稿日期:2019-11-20 出版日期:2020-03-05 发布日期:2020-03-06
作者简介:祖述冲(1983-),男,博士研究生,主要从事森林植物资源中目的活性物质生态分离的研究。

Preparation Process and Bioavailability Evaluation of β-cyclodextrin Inclusion Korean Pine Seed Oil

ZU Shu-Chong

Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040

Received:2019-11-20 Online:2020-03-05 Published:2020-03-06

摘要/Abstract

摘要： 介绍了单因素法优化红松籽油包合物的制备工艺，在最优条件下制备的红松籽油包合物固化率为70.95%，包合物含油率为26.88%。且对红松籽油和红松籽油包合物脂肪酸组分、粒径、电位和生物利用度进行对比。研究结果表明，包合物中红松籽油的各组分含量与红松籽油中各组分含量无明显差异，其中皮诺敛酸的含量为14%~16%。红松籽油包合物的平均粒径为177.3±2.6 nm，电位为-33.01±1.4 mV。红松籽油包合物组的血药最大浓度（Cmax）是红松籽油组的1.27倍，t1/2a是红松籽油的1.42倍；AUC值是红松籽油1.56倍；平均滞留时间（MRT）是红松籽油的1.04倍。因此，通过本实验说明红松籽油包合物与水溶液形成乳剂，粒径减小，水溶性增大，增加了包合物在体内的t1/2a和平均滞留时间（MRT），提高了皮诺敛酸在体内的吸收。

关键词: 红松籽油, 红松籽油包合物, 皮诺敛酸, 生物利用度

Abstract: The single-factor method was used to optimize the preparation process of the Korean pine seed oil inclusion compound. Under the optimal conditions, the solidification rate of the Korean pine seed oil inclusion compound was 70.95%, and the oil content of the inclusion compound was 26.88%. The fatty acid composition, particle size, potential, and bioavailability of Korean pine seed oil and Korean pine seed oil inclusion complex were compared. There was no significant difference between the contents of each component of the Korean pine seed oil in the inclusion compound and that of the Korean pine seed oil, among which the content of pinolenic acid was in 14% to 16%. The average particle size of the Korean pine seed oil inclusion complex was 177.3±2.6 nm, and the potential was -33.01±1.4 mV. The blood drug concentration(CMAX), t1/2A, AUC and MRT were 1.27, 1.42, 1.56 and 1.04 times of the Korean pine oil inclusion complex group, respectively. The inclusion complex of Korean pine seed oil and aqueous solution formed an emulsion, the particle size decreased, the water solubility increased, the inclusion complex in vivo t1/2A and the average retention time(MRT) increased, and the absorption of pinolenic acid in vivo increased.

Key words: Korean pine seed oil, Korean pine seed oil inclusion compound, pinolenic acid, bioavailability

中图分类号:

S791.247

祖述冲. β-环糊精包合红松籽油的制备工艺及生物利用度评价[J]. 植物研究, 2020, 40(2): 314-320.

ZU Shu-Chong. Preparation Process and Bioavailability Evaluation of β-cyclodextrin Inclusion Korean Pine Seed Oil[J]. Bulletin of Botanical Research, 2020, 40(2): 314-320.

参考文献 11

[1]	黄洁,陈丽华,姚振生.《食物本草》中榅桲及海松子植物的考证[J].江西中医学院学报,2003,15(4):54-55.Huang J,Chen L H,Yao Z S.Textual research on cydonia oblonga and haisongzi plant in materia madica of food[J].Journal of Jiangxi University of TCM,2003,15(4):54-55.
[2]	刘国刚.坚果林树种——红松[J].中国林副特产,2004(4):47-48.Liu G G.Tree species of nut forest-Pinus koraiensis[J].Forest By-Product and Speciality in China,2004(4):47-48.
[3]	葛剑平,郭海燕,陈动.小兴安岭天然红松林种群结构的研究[J].东北林业大学学报,1990,18(6):26-32.Ge J P,Guo H Y,Chen D.Study on age structure and spatial pattern of old-growth Korean pine Forest in Lesser Xingan Mountain[J].Journal of Northeast Forestry University,1990,18(6):26-32.
[4]	张秦徽,李蕊,王璧莹,等.红松开花结实研究进展[J].分子植物育种,2019,17(4):1364-1372.Zhang Q H,Li R,Wang B Y,et al.Research progress on flowering and fruiting of Pinus koraiensis[J].Molecular Plant Breeding,2019,17(4):1364-1372.
[5]	王俊国,苗影志,宋玉清,等.长白山木本油料——松籽及松籽油[J].中国油脂,1994,19(6):47-48.Wang J G,Miao Y Z,Song Y Q,et al.Changbai Mountain woody oil-Pine seeds and oils[J].China Oils,1994,19(6):47-48.
[6]	陈永霞.红松籽营养价值分析[J].现代农业科技,2010,(3):361-362.Chen Y X.Analysis on nutritive value of Korean pine seed[J].Modern Agricultural Science and Technology,2010(3):361-362.
[7]	Kim Y M,Kim S,Han T U,et al.Pyrolysis reaction characteristics of Korean pine(Pinus Koraiensis) nut shell[J].Journal of Analytical & Applied Pyrolysis,2014,110:435-441.
[8]	段善海,缪铭.松籽乳饮料的开发研制[J].食品科学,2004,25(6):204-207.Duan S H,Miao M.Developing studies of pine seed milk beverage[J].Food Science,2004,25(6):204-207.
[9]	王振宇,景秋菊.磷酸化改性提高松仁分离蛋白乳化性研究[J].食品工业科技,2006(5):66-68.Wang Z Y,Jing Q J.Phosphorylation of pine nut protein on its emulsification properties[J].Science and Technology of Food Industry,2006(5):66-68.
[10]	饶国华,陈锦屏,张京芳,等.松仁深加工中提高蛋白质提取率工艺研究[J].食品工业科技,2002,23(11):46-47.Rao G H,Chen J P,Zhang J F,et al.Study on improving protein extraction rate in the course of pignolia deep procegsing[J].Science and Technology of Food Industry,2002,23(11):46-47.
[11]	岳红,胡小玲,管萍,等.核桃、松籽营养价值的分析及保健饮品的研制[J].食品科技,1999(2):37-39.Yue H,Hu X L,Guan P,et al.Analysis of nutritional value of walnuts and pine seeds and development of health drinks[J].Food Science and Technology,1999(2):37-39.

β-环糊精包合红松籽油的制备工艺及生物利用度评价

Preparation Process and Bioavailability Evaluation of β-cyclodextrin Inclusion Korean Pine Seed Oil

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