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植物研究 ›› 2020, Vol. 40 ›› Issue (1): 85-92.doi: 10.7525/j.issn.1673-5102.2020.01.013

• 研究报告 • 上一篇    下一篇

胞外三磷酸腺苷对铅胁迫下植物细胞损伤和过氧化氢及其清除酶水平的调节

曹佳鑫, 达梦婷, 庞海龙, 贾凌云, 冯汉青   

  1. 西北师范大学生命科学学院, 兰州 730070
  • 收稿日期:2019-03-13 出版日期:2020-01-05 发布日期:2020-02-21
  • 通讯作者: 冯汉青 E-mail:fenghanq@nwnu.edu.cn
  • 作者简介:曹佳鑫(1990-),女,硕士研究生,主要从事植物生理生态方面研究工作。
  • 基金资助:
    国家自然科学基金(31870246,31560070);甘肃省高等学校科研项目(2015A-007);甘肃省重点研发计划项目(18YF1NA051);甘肃省高校基本科研业务费;西北师范大学青年创新团队项目

Effects of Extracellular adenosine-5'-triphosphate on Cellular damage, H2O2 Content,and Activities of H2O2 Detoxifying Enzymes under Lead Stress

CAO Jia-Xin, DA Meng-Ting, PANG Hai-Long, JIA Ling-Yun, FENG Han-Qing   

  1. College of Life Science, Northwest Normal University, Lanzhou 730070
  • Received:2019-03-13 Online:2020-01-05 Published:2020-02-21
  • Supported by:
    National Natural Science Foundation of China(31870246,31560070);University Scientific Research Project of Gansu Province(2015A-007);Key Research and Development Project of Gansu Province(18YF1NA051);Fundamental Research Funds for the Gansu Universities of Gansu Provincial Department of Finance;Youth Innovation Team of Northwest Normal University

摘要: 细胞外三磷酸腺苷(extracellular adenosine-5'-triphosphate)是植物细胞的重要信号分子。以烟草悬浮细胞BY-2(Nicotiana tabacum L.cv.Bright Yellow-2)为材料,探讨了胞外三磷酸腺苷对铅胁迫下细胞损伤、H2O2(过氧化氢)含量及H2O2清除酶活性的影响。结果显示,随着Pb(NO32浓度的不断提高(30~400 μmol·L-1),细胞外三磷酸腺苷含量呈现出逐渐下降的趋势,但胞内三磷酸腺苷含量及细胞的受损伤程度逐渐增大;同时,H2O2含量和过氧化氢酶的活性均有所上升,并在200 μmol·L-1 Pb(NO32处理下达到最大值,而过氧化物酶的活性则不断降低。较之Pb(NO32胁迫下的细胞,对Pb(NO32胁迫的细胞加入外源三磷酸腺苷使得细胞受损伤程度显著降低,H2O2含量减少,过氧化氢酶活性减弱,而过氧化物酶活性增强。实验结果表明,Pb(NO32胁迫诱导的植物细胞损伤和H2O2及其清除酶水平的变化能受到细胞外三磷酸腺苷水平的调节。

关键词: 细胞损伤, 胞外三磷酸腺苷, 铅胁迫, 过氧化氢(H2O2), 过氧化氢清除酶

Abstract: The extracellular adenosine-5'-triphosphate is an important signaling molecule for plant cells. By using the tobacco(Nicotiana tabacum L.cv.Bright Yellow-2) cell suspension cultures, we studied the effects of extracellular adenosine-5'-triphosphate on the cellular damage, H2O content, and the activities of H2O detoxifying enzymes underlead stress. With the increase of concentrations of Pb(NO3)2(from 30-400 μmol·L-1), the levels of the extracellular adenosine-5'-triphosphate were decreased, whilethe levels of the intracellular adenosine-5'-triphosphate and cellular damage were increased. The content of H2O2 and the activity of the CAT(catalaseenzyme) were increased and reached their maximum at 200 μmol·L-1Pb(NO3)2, while the activityof the POD(peroxide enzyme) was decreased, with the increase of concentrations of Pb(NO3)2. Compared with the cells under lead(200 μmol·L-1) stress, the lead-stressed cells treated with exogenous adenosine-5'-triphosphate(30 μmol·L-1) had lower levels of cellular damage, H2O2 content, andthe activity of CAT, but had higher level of the activity of POD. Therefore, the cellular damage, H2O content, and the activities of H2O detoxifying enzymesunderlead stress could beregulated by extracellular adenosine-5'-triphosphate.

Key words: cellular damage, extracellular adenosine-5'-triphosphate, lead stress, H2O2, H2O detoxifying enzymes

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