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植物研究 ›› 2019, Vol. 39 ›› Issue (2): 287-293.doi: 10.7525/j.issn.1673-5102.2019.02.015

• 研究报告 • 上一篇    下一篇

白桦BpBEE2基因的遗传转化及抗逆性分析

颜斌, 武丹阳, 李慧玉   

  1. 东北林业大学林木遗传育种国家重点实验室, 哈尔滨 150040
  • 收稿日期:2018-11-05 出版日期:2019-03-05 发布日期:2019-03-20
  • 通讯作者: 李慧玉 E-mail:lihuiyu2017@126.com
  • 作者简介:颜斌(1992-),男,硕士研究生,主要从事林木遗传育种研究。
  • 基金资助:
    国家重点研发计划(2017YFD0600603)

Genetic Transformation and Resistance Analysis of BpBEE2 Gene from Betula platyphylla

YAN Bin, WU Dan-Yang, LI Hui-Yu   

  1. State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040
  • Received:2018-11-05 Online:2019-03-05 Published:2019-03-20
  • Supported by:
    National Key R&D Plan(2017YFD0600603)

摘要: Brassinolide Enhanced Expression2(BEE2)基因属于bHLH转录因子家族,是调控油菜素内酯信号转导的上游调控因子。本研究通过RT-PCR技术克隆BpBEE2基因的全长cDNA序列,构建植物过表达及抑制表达载体,并通过农杆菌介导法进行白桦的遗传转化,对获得的转基因株系进行生长量及盐、旱胁迫分析,结果表明:获得了长度为1 080 bp的全长cDNA序列,成功构建了该基因的过表达及抑制表达载体,并获得了过表达和抑制表达的白桦株系。BpBEE2基因过表达白桦株系的苗高高于对照株系,而抑制表达株系的苗高低于对照株系。同时发现BEE2基因对盐、旱胁迫后对植株的鲜重也产生了影响。说明BpBEE2可能参与了植物的生长发育过程,并且改善了植物的抗旱、耐盐性。

关键词: 白桦, BpBEE2基因, 载体构建, 遗传转化, 抗旱耐盐

Abstract: Brassinolide Enhanced Expression2(BEE2) genes belong to bHLH transcription factors family,and were involved in Brassinolide signal pathway.In this study, BpBEE2 gene was cloned using RT-PCR, and plant over-expression and inhibiting expression vector were constructed. Genetic transformation was operated through agrobacterium mediated, then mass growths of transgenic lines and non-transgenic line were analyzed under natural conditions and salt, drought stress. The length of 1 080 bp cDNA sequence was obtained, over-expression and inhibiting expression vectors were constructed successfully, and transgenic lines were obtained. Seedlings height of over-expression transgenetic line was higher than that of the control line, however, that of inhibiting expression lines was lower than that of the control line. BEE2 gene can ffect fresh weight of plants after salt and drought stress, and BpBEE2 is involved in the process of plant growth and development and plays a key role in drought and salt resistance response.

Key words: Betula platyphylla, BpBEE2 gene, vector construction, genetic transformation, drought and salt resistance

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