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植物研究 ›› 2018, Vol. 38 ›› Issue (6): 913-920.doi: 10.7525/j.issn.1673-5102.2018.06.015

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水曲柳FmMUR5基因的克隆及表达模式的分析

王恒涛, 邵婉璇, 徐舒浩, 曾凡锁   

  1. 东北林业大学生命科学学院, 哈尔滨 150040
  • 收稿日期:2018-05-14 出版日期:2018-11-15 发布日期:2018-11-01
  • 通讯作者: 曾凡锁 E-mail:zengfansuo@126.com
  • 作者简介:王恒涛(1995-),男,本科生,主要从事植物逆境分子生物学方面的研究。
  • 基金资助:
    东北林业大学大学生创新项目(201710225302)

Cloning and Expression Pattern Analysis of MUR5 Gene in Fraxinus mandshurica

WANG Heng-Tao, SHAO Wan-Xuan, XU Shu-Hao, ZENG Fan-Suo   

  1. College of Life Sciences, Northeast Forestry University, Harbin 150040
  • Received:2018-05-14 Online:2018-11-15 Published:2018-11-01
  • Supported by:
    University of Northeastern Forestry Undergraduate Innovation Project(201710225302)

摘要: 在核苷糖合成途径中,尿苷二磷酸-阿拉伯吡喃糖变位酶(MUR5)可以催化UDP-阿拉伯吡喃糖和UDP-阿拉伯呋喃糖之间的相互转化。本研究分析了水曲柳FmMUR5基因的时空表达模式及其在非生物胁迫和植物激素诱导下的表达特征。结果表明,水曲柳FmMUR5编码区全长为1 080 bp,编码359个氨基酸。FmMUR5蛋白为稳定的亲水性蛋白,不存在信号肽和跨膜结构。FmMUR5基因与樟子松、大麦等物种的MUR5遗传距离比较近,说明它们亲缘关系比较近。FmMUR5基因在新生枝中表达最高,具有组织特异性,各个部位在八月份表达量相对较高。非生物胁迫和激素诱导下,FmMUR5基因的表达量随处理时间而改变,但其变化趋势不尽相同。在低温(4℃)、盐(NaCl)以及甘露醇3种非生物胁迫下,FmMUR5基因在处理1 h后表达量最高,分别为对照组的1.5、1.4和6.3倍;在4℃和甘露醇处理24 h后,FmMUR5基因表达量最低,而在盐(NaCl)处理12 h后表达量最低。FmMUR5基因在脱落酸(ABA)和生长素(IAA)处理1 h后表达量最高,分别为对照组的2.5和3.8倍,在赤霉素(GA)处理12 h后表达量最高,为对照组的1.1倍;而FmMUR5基因在脱落酸(ABA)处理24 h后表达量最低,在生长素(IAA)和赤霉素(GA)处理6 h后表达量最低。本研究结果表明FmMUR5基因参与非生物胁迫以及植物激素信号诱导。

关键词: 水曲柳, FmMUR5, 生物信息学分析, 非生物胁迫

Abstract: WE studied the expression pattern of FmMUR5 in different phases and abiotic stress, and revealed the phytohormone-induced expression characteristics. We bioinformatically analyzed the FmMUR5 gene and the encoded protein in Fraxinus mandshurica. The coding region of FmMUR5 is 1 080 bp in length and encodes 359 amino acids. The FmMUR5 protein is a stable, hydrophilic protein in the absence of signal peptide and transmembrane structure. The genetic distance between FmMUR5 and Pinus sylvestris var. mongolica and Hordeum vulgare was relatively close, indicating that they are relatively close genetic relationship. The expression of FmMUR5 gene was the highest in newly formed shoots with tissue specificity, and the expression of FmMUR5 in each part was relatively high in August. Under abiotic stress the induction of hormone signal, the expression level of FmMUR5 gene changed with the time of treatment, but the trend was not the same. FmMUR5 gene expression was highest by treated for 1 h at low temperature (4℃), salt (NaCl) and mannitol three abiotic stresses, 1.5, 1.4 and 6.3 times of the control group, respectively. FmMUR5 was the lowest at 4℃ and 24 h after mannitol treatment, while FmMUR5 was the lowest at 12 h after salt treatment (NaCl). The expression level of FmMUR5 gene was the highest at 1 h after abscisic acid (ABA) and auxin (IAA) treatment and 12 h after gibberellin (GA) treatment, 2.5, 3.8 and 1.1 times of the control group, respectively. The expression level of FmMUR5 was the lowest at 24 h after abscisic acid (ABA) treatment and lowest at 6 hours after treatment with IAA and GA. The results indicate that FmMUR5 is involved in abiotic stress and plant hormone signal induction.

Key words: Fraxinus mandshurica, FmMUR5, bioinformatics analysis, abiotic stress

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