欢迎访问《植物研究》杂志官方网站,今天是 分享到:

植物研究 ›› 2018, Vol. 38 ›› Issue (5): 675-681.doi: 10.7525/j.issn.1673-5102.2018.05.006

• 研究报告 • 上一篇    下一篇

稀土元素对短叶对齿藓生理生化的研究

高叶青, 任冬梅   

  1. 内蒙古大学生命科学学牧草和特色作物生物技术教育部重点实验室, 呼和浩特 010070
  • 收稿日期:2018-04-10 出版日期:2018-09-15 发布日期:2018-08-31
  • 通讯作者: 任冬梅 E-mail:rendongmei1970@aliyun.com
  • 作者简介:高叶青(1991-),女,硕士研究生,主要从事苔藓植物组织培养。
  • 基金资助:
    国家自然科学基金(31360142,31260046);内蒙古大学高层次人才引进项目

Influence of Rare Earth Elements on Physiological and Biochemical Characteristics of Didymodon tectorum

GAO Ye-Qing, REN Dong-Mei   

  1. College of Life Science, Inner Mongolia University and Key Laboratory of Forage and Endemic Crop Biotechnology, Ministry of Education, School of Life Sciences, Inner Mongolia University, Hohhot 010070
  • Received:2018-04-10 Online:2018-09-15 Published:2018-08-31
  • Supported by:
    National Natural Science Foundation of China(31360142,31260046);High Level Talent Introduction Project of Inner Mongolia University

摘要: 通过测定分析3个主要生理指标过氧化物酶(POD)活性、丙二醛(MDA)含量和叶绿素含量的变化,研究了短叶对齿藓组培苗在6个不同浓度梯度的轻稀土La3+,Ce4+和重稀土Y3+单一元素胁迫下的生理响应和变化。结果如下:(1)短叶对齿藓体内的过氧化物酶(POD)活性和丙二醛(MDA)含量各处理组均低于对照组;Ce4+元素在3.55×10-2 mmol·L-1时显著提高了POD活性,在7.1×10-2 mmol·L-1时显著增加了膜脂过氧化产物丙二醛(MDA)含量。表明短叶对齿藓对Ce4+元素胁迫响应较强;而La3+元素各处理浓度间POD的变化较为平缓,胁迫响应较弱;Y3+处理居中。(2)较低浓度的La3+(1.8×10-2 mmol·L-1)和Ce4+(3.55×10-2 mmol·L-1)时显著提高叶绿素a、叶绿素b和总叶绿素含量;3种稀土元素在较高浓度时叶绿素的含量均有明显下降。本研究为进一步探明白云鄂博稀土矿区的苔藓植物生长发育受稀土元素的影响奠定了基础。

关键词: 短叶对齿藓, 稀土元素, 酶活性, 丙二醛, 叶绿素

Abstract: Based on the successful application of tissue cultured seedlings of Didymodon tectorum, three physiological indexes, including POD vitality, MDA and chlorophyll content were determined to check out the influences of the rare earth elements stress under gradient concentrations treatment. We choosed the light rare earth La3+, Ce4+ and heavy rare earth Y3+ three elements and setted respectively six concentrations under the single elemnet stress, in order to explore the physiological response and changes of D.tectorum to the rare earth stress. The results were the following:(1)The peroxidase(POD) activity and malondialdehyde(MDA) content in D.tectorum were lower in each treatment group than those in the control group; Ce4+ element significantly promoted the POD activity at 3.55×10-2 mmol·L-1, at 7.1×10-2 mmol·L-1, the content of MDA increased. It showed that the D.tectorum had a stronger response to Ce4+ stress, while La3+ showed a more gradual change in POD activity, and the stress response was weaker; Y3+ treatment was intermediate; (2)At lower concentrations of La3+(1.8×10-2 mmol·L-1) and Ce4+(3.55×10-2 mmol·L-1) the contents of chlorophyll a, chlorophyll b and total chlorophyll increased significantly, but at higher concentrations of all three rare earth elements, the contents of chlorophyll decreased obviousily. This study laid the foundation for further exploration of the effects of rare earth elements on the growth and the development of bryophytes in the Baiyun obo rare earth mineral area.

Key words: Didymodon tectorum, rare earth elements, enzyme activity, malondialdehyde, chlorophyll

中图分类号: