欢迎访问《植物研究》杂志官方网站,今天是 分享到:

植物研究 ›› 2012, Vol. 32 ›› Issue (5): 632-635.doi: 10.7525/j.issn.1673-5102.2012.05.022

• 论文 • 上一篇    下一篇

山东省长岛县南部四岛藜(Chenopodium album L.)天然种群遗传多样性的ISSR分析

张恒庆1;房孝迪1;吴锦美1;杜洋1;谭琨1;范强军2;祝恩伟2   

  1. 1.辽宁师范大学生命科学学院,大连 116029;2.山东长岛国家级自然保护区管理局,长岛 265800
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-09-20 发布日期:2012-09-20
  • 基金资助:
     

Analysis on Genetic Diversity of Chenopodium album L. Populations in Four Natural Islands of South of Changdao,Shandong Province by ISSR

ZHANG Heng-Qing;FANG Xiao-Di;WU Jin-Mei;DU Yang;TAN Kun;FAN Qiang-Jun;ZHU En-Wei   

  1. 1.College of Life Sciences,Liaoning Normal University,Dalian 116029;2.Shandong Changdao National Nature Reserve Administration,Changdao 265800
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-09-20 Published:2012-09-20
  • Supported by:
     

摘要: 采用ISSR分子标记对分布于山东省长岛县南部的北长山、南长山、大黑山和小黑山4个岛屿上的藜天然种群共81个个体进行了遗传多样性及遗传结构的研究。13个引物共检测到157个可重复的位点。遗传多样性研究结果表明:种内的多态位点比率为66.24%,具有较高的遗传多样性(Shannon(I)指数在物种水平上为0.332 0);种群间有一定的遗传分化,根据Gst值,种群间的遗传多样性占总群体的9.27%。遗传距离分析表明,XHS种群和NCS种群的遗传一致度最高,与地理距离无相关性。

关键词: 藜, 遗传多样性, 遗传结构, ISSR

Abstract: Using ISSR method, the genetic diversity and genetic structure of 81 individuals in four natural populations of Chenopodium album L. distributed in four islands in Changdao, Shandong Province, i.e. Beichangshan island, Nanchangshang island, Daheishan island and Xiaoheishan island were studied in this paper. Through the amplification of ISSR with 13 primers, 157 loci were detected. The results showed that the percentage of polymorphic loci was 66.24% with a high genetic diversity (Shannon’s information index was 0.332 0 at the species level); genetic differentiation existed among populations (Nei’s gene diversity was 0.220 1 at the species level); at the species level, genetic variance was 9.27% among populations according to GST value. The genetic distance analysis showed that XHS population and NCS population have the highest population genetic identity, genetic distances and geographic distances have no correlation.

Key words: Chenppodium album L., genetic diversity, genetic structure, ISSR

中图分类号: