不同时期大叶苦丁茶(Ilex latifolia Thunb.)不同器官中咖啡酰奎宁酸类成分的毛细管电泳分析

doi:10.7525/j.issn.1673-5102.2018.03.018

植物研究 ›› 2018, Vol. 38 ›› Issue (3): 453-459.doi: 10.7525/j.issn.1673-5102.2018.03.018

不同时期大叶苦丁茶(Ilex latifolia Thunb.)不同器官中咖啡酰奎宁酸类成分的毛细管电泳分析

林春松, 徐夙侠, 黄青云

福建省亚热带植物研究所植物生理生化重点实验室, 厦门 361006

收稿日期:2017-08-01 出版日期:2018-05-15 发布日期:2018-05-17
作者简介:林春松(1975-), male, associate professor, Major in utilization of plant resources.
基金资助:
The Xiamen Municipal Science & Technology Project(grant numbers.3502Z20162009);Xiamen Great Plantform of science & Technology(3502Z20131004)

Caffeoylquinic Acid Derivatives in DAYE Kudingcha(Ilex latifolia Thunb.) Organs in Differently Developing Stage by Micellar Electrokinetic Chromatography

LIN Chun-Song, XU Su-Xia, HUANG Qing-Yun

Key Laboratory of Physiology and Biochemistry, Fujian Institute of Subtropical Botany, 800 Jiahe Road, Xiamen 361006

Received:2017-08-01 Online:2018-05-15 Published:2018-05-17
Supported by:
The Xiamen Municipal Science & Technology Project(grant numbers.3502Z20162009);Xiamen Great Plantform of science & Technology(3502Z20131004)

摘要/Abstract

摘要： 大叶苦丁茶（Ilex latifolia Thunb.）含有丰富的咖啡酰奎宁酸。在本研究中，运用胶束电动毛细管色谱法，测定了在不同发育时期的大叶苦丁茶不同器官中的咖啡酰奎宁酸含量。大叶苦丁茶的不同器官在不同发育时期的咖啡酰奎宁酸含量有着显著的变化。在叶片中，10天叶龄的叶的咖啡酰奎宁酸含量是19.78 g/100 g，而当叶龄达到12月时，其中的咖啡酰奎宁酸含量已经降为1.41 g/100 g。随着发育时间的增长，雌花、雄花中的含量变化也有类似的变化。雌花（果）和雄花在发育期为10天时的咖啡酰奎宁酸含量高：分别20.29 g/100 g和18.06 g/100 g，随后在雌花发育成的7个月果实中下降到0.16 g/100 g，而在40天期接近脱落的雄花中其含量降为10.36 g/100 g。大叶苦丁茶的器官中，除了嫩叶，雄花由于高含量的咖啡酰奎宁酸和较多的生物量，也是一种好的咖啡酰奎宁酸来源材料。本文首次报道了大叶苦丁茶的花中含有大量的咖啡酰奎宁酸。MEKC方法也是首次被用于苦丁茶中的咖啡酰奎宁酸类成分的测定，使用这种方法，大叶苦丁茶中的6种咖啡酰奎宁酸成分在40分钟内完全分离。相较于以前文献中报道的高效液相色谱法，本文为苦丁茶中的咖啡酰奎宁酸的质量控制提供了一种新的检测方法。

关键词: 咖啡酰奎宁酸衍生物, 大叶苦丁茶(Ilex latifolia Thunb.), MEKC, 年龄, 花, 叶片

Abstract: DAYE Kudingcha is rich in caffeoylquinic acid derivatives(CQAs). CQAs in DAYE Kudingcha organs in different developing stages were determined by micellar electrokinetic chromatography(MEKC). The composition of CQAs variated greatly in tissues at differently developing stages. The content of CQAs was 19.78 g/100 g in leaves at the age of 10 days, and then decreased to 1.41 g/100 g at 12 months. A similar predisposition was observed in female and male flowers with the increasing age. Female flowers(fruits) and male flowers at the age of 10 days have a high content of CQAs:20.29 g/100 g and 18.06 g/100 g, respectively, which subsequently decreased to 0.16 g/100 g in 7-month mature fruits, 10.36 g/100 g in 40 day falling male flowers. The male flowers, as well as the young leaves, might be a good CQAs' raw material, due to a large amount of CQAs and biomass of the male flowers in the Kudingcha species. This is the first report describing the contents of CQAs in the flowers of Kudingcha. MEKC method was also used for the first time in the determination of CQAs in Kudingcha. Compared to the HPLC method described previously, the MEKC might be an alternative for the quality control of CQAs in Kudingcha.

Key words: caffeoylquinic acid derivatives, Ilex latifolia Thunb., MEKC, age, flowers, leaves

中图分类号:

S571.1

林春松, 徐夙侠, 黄青云. 不同时期大叶苦丁茶(Ilex latifolia Thunb.)不同器官中咖啡酰奎宁酸类成分的毛细管电泳分析[J]. 植物研究, 2018, 38(3): 453-459.

LIN Chun-Song, XU Su-Xia, HUANG Qing-Yun. Caffeoylquinic Acid Derivatives in DAYE Kudingcha(Ilex latifolia Thunb.) Organs in Differently Developing Stage by Micellar Electrokinetic Chromatography[J]. Bulletin of Botanical Research, 2018, 38(3): 453-459.

参考文献

1. 谢观,樊正伦,张年顺.中国医学大辞典[M].北京:中国中医药出版社,1994. Xie G,Fan Z L,Zhang L X.Grand dictionary of Chinese medicine[M].Beijing:Beijing Traditional Chinese Medicine Press,1994.
2. Hu T,He X W,Jiang J G.Functional analyses on antioxidant,anti-inflammatory,and antiproliferative effects of extracts and compounds from Ilex latifolia Thunb.,a Chinese bitter tea[J].Journal of Agricultural and Food Chemistry,2014,62(34):8608-8615.
3. Hu T,He X W,Jiang J G,et al.Efficacy evaluation of a Chinese bitter tea(Ilex latifolia Thunb.) via analyses of its main components[J].Food & Function,2014,5(5):876-881.
4. Shahrzad S,Bitsch I.Determination of some pharmacologically active phenolic acids in juices by high-performance liquid chromatography[J].Journal of Chromatography A,1996,741(2):223-231.
5. Farah A,De Paulis T,Moreira D P,et al.Chlorogenic acids and lactones in regular and water-decaffeinated arabica coffees[J].Journal of Agricultural and Food Chemistry,2006,54(2):374-381.
6. Thuong P T,Su N D,Ngoc T M,et al.Antioxidant activity and principles of Vietnam bitter tea Ilex kudingcha[J].Food Chemistry,2009,113(1):139-145.
7. Song C W,Xie C,Zhou Z W,et al.Antidiabetic effect of an active components group from Ilex kudingcha and its chemical composition[J].Evidence-Based Complementary and Alternative Medicine,2012,2012:423690.
8. Negishi O,Negishi Y,Yamaguchi F,et al.Deodorization with ku-ding-cha containing a large amount of caffeoyl quinic acid derivatives[J].Journal of Agricultural and Food Chemistry,2004,52(17):5513-5518.
9. Midorikawa M,Ohnishi-Kameyama M,Nagata T.Seasonal difference of caffeic acid derivative contents in current-year leaves and old leaves of Ilex latifolia Thunb[J].Journal for the Integrated Study of Dietary Habits,2010,20(4):305-312.
10. 江苏新医学院.中药大辞典[M].上海:上海科学技术出版社,1985. Jiangsu College of New Medicine.Dictionary of Chinese medicine[M].Shanghai:Shanghai Press of Science and Technology,1985.
11. 中国科学院中国植物志编辑委员会.中国植物志[M].北京:科学出版社,1999:107-110. Academicae Sinicae Edita.Flora of China[M].Beijing:Science Press,1999:107-110.
12. Marques V,Farah A.Chlorogenic acids and related compounds in medicinal plants and infusions[J].Food Chemistry,2009,113(4):1370-1376.
13. Liu L X,Sun Y,Laura T,et al.Determination of polyphenolic content and antioxidant activity of kudingcha made from Ilex kudingcha C.J.Tseng[J].Food Chemistry,2009,112(1):35-41.
14. Chu Q C,Lin M,Tian X H,et al.Study on capillary electrophoresis-amperometric detection profiles of different parts of Morus alba L.[J].Journal of Chromatography A,2006,1116(1-2):286-290.
15. 徐夙侠,林春松,黄青云,等.3个三角梅品种中类黄酮的毛细管电泳分析比较研究[J].植物研究,2010,30(6):718-724. Xu S X,Lin C S,Huang Q Y,et al.Analysis and comparison of flavonoids in three bougainvilleas by Micellar Electrokinetic Chromatography[J].Bulletin of Botanical Research,2010,30(6):718-724.
16. De Paulis T,Commers P,Farah A,et al.4-Caffeoyl-1,5-quinide in roasted coffee inhibits[³H] naloxone binding and reverses anti-nociceptive effects of morphine in mice[J].Psychopharmacology,2004,176(2):146-153.
17. Farah A,De Paulis T,Trugo L C,et al.Effect of roasting on the formation of chlorogenic acid lactones in coffee[J].Journal of Agricultural and Food Chemistry,2005,53(5):1505-1513.
18. 王明彥.苦丁茶中二咖啡酰基奎宁酸对人肿瘤细胞的体外抑制作用及其机制研究[D].武汉:湖北中医药大学,2014. Wang M Y.The inhibitory effects of dicaffeoylquinic acid from ilex kudingcha on human cancer cells and its mechanism of action in vitro[D].Wuhan:Hubei University of Chinese Medicine,2014.
19. Dartora N,De Souza L M,Santana-Filho A P,et al.UPLC-PDA-MS evaluation of bioactive compounds from leaves of Ilex paraguariensis with different growth conditions,treatments and ageing[J].Food Chemistry,2011,129(4):1453-1461.
20. Farag M A,Porzel A,Wessjohann L A.Comparative metabolite profiling and fingerprinting of medicinal licorice roots using a multiplex approach of GC-MS,LC-MS and 1D NMR techniques[J].Phytochemistry,2012,76:60-72.
21. Butiuk A P,Martos M A,Adachi O,et al.Study of the chlorogenic acid content in yerba mate(Ilex paraguariensis St.Hil.):Effect of plant fraction,processing step and harvesting season[J].Journal of Applied Research on Medicinal and Aromatic Plants,2016,3(1):27-33.

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不同时期大叶苦丁茶(Ilex latifolia Thunb.)不同器官中咖啡酰奎宁酸类成分的毛细管电泳分析

Caffeoylquinic Acid Derivatives in DAYE Kudingcha(Ilex latifolia Thunb.) Organs in Differently Developing Stage by Micellar Electrokinetic Chromatography

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