欢迎访问《植物研究》杂志官方网站,今天是 分享到:

植物研究 ›› 2018, Vol. 38 ›› Issue (2): 284-291.doi: 10.7525/j.issn.1673-5102.2018.02.017

• 研究报告 • 上一篇    下一篇

外源乙烯对长春花生理水平和生物碱积累的影响

常博文1, 刘杰1, 钟鹏1, 郭晓瑞2   

  1. 1. 黑龙江省农业科学院农村能源研究所, 哈尔滨 150040;
    2. 东北林业大学森林植物生态学教育部重点实验室, 哈尔滨 150040
  • 收稿日期:2017-09-18 出版日期:2018-03-15 发布日期:2018-03-23
  • 通讯作者: 郭晓瑞 E-mail:xruiguo@nefu.edu.cn
  • 作者简介:常博文(1986-),女,博士,主要从事育种和植物生理相关研究工作。
  • 基金资助:
    国家自然科学基金青年基金项目“长春花生物碱生物合成受钾元素调控的代谢和分子基础”(31400337)

Effects of Exogenous Ethylene on Physiology and Alkaloid Accumulations in Catharanthus roseus

CHANG Bo-Wen1, LIU Jie1, ZHONG Peng1, GUO Xiao-Rui2   

  1. 1. Heilongjaing Academy of Agricultural Science Rural Energy Institute, Harbin 150040;
    2. Key Laboratory of Forest Plant Ecology of Northeast Forestry University, Ministry of Education, Harbin 150040
  • Received:2017-09-18 Online:2018-03-15 Published:2018-03-23
  • Supported by:
    National Natural Science Foundation of China Youth Fund Project "The metabolism and molecular basis of potassium elements regulating biosynthesis of alkaloids in Catharanthus roseus"(31400337)

摘要: 药用植物长春花中含有100多种萜类吲哚生物碱(TIAs),其中具有抗肿瘤功效的长春碱和长春新碱受到关注。为了研究外源乙烯处理对长春花生长情况、生理状态和萜类吲哚生物碱合成的整体影响,本文以长春花幼苗为实验材料,使用外源乙烯处理后对比了不同生长条件下长春花的生物量积累、根茎伸长、光合参数以及生物碱含量等指标,分析了生物碱合成与其他指标之间的相关性。结果表明,外源乙烯处理使长春花乙稀释放量上升,乙烯信号响应因子erf基因表达量提高。乙烯利抑制长春花幼苗生物量积累、根纵向生长,促进茎秆横向加粗生长,由非气孔因素导致净光合速率(Pn)和气孔导度(Gs)下降。外源乙烯促进异胡豆苷(STR)、长春质碱(CAT)、文多灵(VIN)和长春碱(VINB)积累,并且促进长春碱合成途径中关键酶基因strCrPRX上调表达。相关性分析结果表明,次生代谢产物的积累、生长指标、光合参数之间存在明显的相关性;长春质碱、文多灵、长春碱与茎秆直径(SD)显著正相关(P < 0.05),与生物量(B)、株高(H)、根长(RL)、净光合速率(Pn)呈显著负相关(P < 0.05)。本文为研究外源乙烯调控长春花生物碱积累的机制提供理论基础。

关键词: 乙烯利, 长春花, 生理水平, 生物碱积累, 相关性分析

Abstract: Catharanthus roseus, as a medicinal plant, contains more than 100 terpenoid indole alkaloids(TIAs), including vinblastine and vincristine which focus on anti-cancer effects. In order to study the comprehensive influence of exogenous ethylene on growth, physiology, and terpenoid indole alkaloid accumulations, C.roseus seedlings were cultured as experimental material and contrasted the data of biomass, growth rate of root and stem, photosynthetic parameter and alkaloid contents with or without ethephon treatment, and the correlations between these indexes were analyzed. The ethylene release amount and the expression of erf(ethylene-response factor) gene were increased under exogenous ethylene treatment. Ethephon inhibited biomass, longitudinal growth but promoted lateral thickening growth of stem; in addition, net photosynthetic rate(Pn) and stomatal conductance(Gs) were decreased leading from non-stomatal factor. The biosynthesis of four alkaloids including strictosidine(STR), catharanthine(CAT), vindoline(VIN) and vinblastine(VINB) were accelerated in ethephon-treatment group, and the expression of key enzyme genes(str and CrPRX) were up-regulated by ethephon. By correlation analysis, we found that relationships among alkaloid contents, growth indexes and photosynthetic parameters were significant and photosynthetic parameters were significant. It were positive relation between three alkaloids including CAT, VIN, VINB and stem diameter(SD)(P < 0.05). On the contrary, there was a significantly negative relations between these three alkaloids and biomass(B), height(H), root length(RL), net photosynthetic rate(Pn)(P < 0.05). Our study will provide useful information for mechanism research of exogenous ethylene regulating TIAs biosynthesis in C.roseus.

Key words: ethephon, Catharanthus roseus, physiological level, alkaloid accumulation, correlation analysis

中图分类号: