欢迎访问《植物研究》杂志官方网站,今天是 分享到:

植物研究 ›› 2018, Vol. 38 ›› Issue (2): 268-277.doi: 10.7525/j.issn.1673-5102.2018.02.015

• 研究报告 • 上一篇    下一篇

2个慈竹bZIP基因的克隆、生物信息学分析及其诱导表达

龚道勇1,2, 胡尚连1,2, 曹颖1,2, 卢学琴1,2, 张庆波1,2   

  1. 1. 西南科技大学植物细胞工程实验室, 绵阳 621010;
    2. 四川省生物质资源利用与改性工程技术研究中心, 绵阳 621010
  • 收稿日期:2017-06-06 出版日期:2018-03-15 发布日期:2018-03-23
  • 通讯作者: 胡尚连 E-mail:hushanglian@126.com
  • 作者简介:龚道勇(1990-),男,硕士研究生,主要从事植物遗传与品种改良研究。
  • 基金资助:
    国家自然科学基金(31400333,31400257);四川省“十三五”重点攻关项目(2016NYZ0038);四川省重点研发项目(2017NZ0008);西南科技大学研究生创新基金项目(17ycx086)

Cloning and Bioinformatics Analysis of Two bZIP Genes of Bambusa emeiensis and Their Induced Expression under Abiotic Stresses

GONG Dao-Yong1,2, HU Shang-Lian1,2, CAO Ying1,2, LU Xue-Qin1,2, ZHANG Qing-Bo1,2   

  1. 1. Lab of Plant Cell Engineering Southwest University of Science and Technology, Mianyang 621010;
    2. Engineering Research Center for Biomass Resource Utilizaiton and Modification of Sichuan Province, Mianyang 621010
  • Received:2017-06-06 Online:2018-03-15 Published:2018-03-23
  • Supported by:
    National Natural Science Foundation of China(31400333,31400257);Sichuan Province's 13th Five Key Breeding Project(2016NYZ0038);Key Research and Development Project of Sichuan Province, China(2017NZ0008);Graduate Innovation Project of Southwest University of Science and Technology(17ycx086)

摘要: 从慈竹笋转录组数据库中筛选并克隆出2个bZIP基因(BebZIP2和BebZIP6)进行生物信息学分析。分析结果表明,它们编码序列长度分别为504和720 bp,编码167个和239个氨基酸,BebZIP2和BebZIP6属于bZIP相关蛋白,与水稻OsbZIP52/RISBZ5蛋白聚在一枝。组织表达分析表明,这2个慈竹BebZIP基因在慈竹笋、茎、展开叶和未展开叶等部位均有表达,属于组成型表达,同一基因在不同组织中的表达量差异较大,表达量的大小为未展开叶 > 展开叶 > 茎 > 笋。对慈竹幼苗进行ABA、NaCl和PEG6000非生物胁迫处理,结果表明,BebZIP2和BebZIP6对盐、干旱和ABA胁迫均具有不同程度的响应。

关键词: 慈竹, bZIP转录因子, 生物信息学分析, 组织表达分析, 非生物胁迫

Abstract: According to the transcriptome data of Bambusa emeiensis shoot, two bZIP genes designated as BebZIP2 and BebZIP6 were cloned and their bioinformatics were analyzed. The bioinformatics analysis results showed that the full-length cDNA sequence of BebZIP2 and BebZIP6 were 504 and 720 bp, and encoded 167 and 239 amino acids, respectively. BebZIPs and rice OsbZIP52/RISB5 proteins were clustered into the same branch, which were bZIP-related proteins. The tissue expression patterns of two BebZIP genes were analyzed by real-time PCR. Two BebZIP gene sequences were expressed in all shoots, stalks, unfolding leaves and rolled leaves of B.emeiensis. There were the expression differences of the same gene in the different tissues and expression quantity in the descending order was unfolding leaves, rolled leaves, stalks and shoots. The seedlings of B.emeiensis were treated with ABA, NaCl and PEG 6000 for abiotic stress analysis. The expression level of BebZIP2 and BebZIP6 genes treated by salt, drought and ABA stresses have different degree of sensitivity.

Key words: Bambusa emeiensis, bZIP transcription factors, bioinformatics analysis, tissue expression analysis, abiotic stresses

中图分类号: