交替呼吸途径对CuCl2胁迫下菜豆叶片光系统Ⅱ的保护作用

doi:10.7525/j.issn.1673-5102.2018.01.009

植物研究 ›› 2018, Vol. 38 ›› Issue (1): 75-80.doi: 10.7525/j.issn.1673-5102.2018.01.009

交替呼吸途径对CuCl₂胁迫下菜豆叶片光系统Ⅱ的保护作用

谢佳佳, 芦丽娜, 石岱龙, 王庆文, 贾凌云, 冯汉青

西北师范大学生命科学学院, 兰州 730070

收稿日期:2017-04-05 出版日期:2018-01-15 发布日期:2018-01-06
通讯作者: 冯汉青,E-mail:fenghanq@nwnu.edu.cn E-mail:fenghanq@nwnu.edu.cn
作者简介:谢佳佳(1993—),女,硕士研究生,主要从事植物生理生态方面研究工作.
基金资助:
国家自然科学基金（31560070）；甘肃省高等学校科研业务费；甘肃省高等学校科研项目；西北师范大学创新团队项目

Protective Effects of Alternative Respiratory Pathway on Photosystem Ⅱ in Phaseolus vulgaris under CuCl₂ Stress

XIE Jia-Jia, LU Li-Na, SHI Dai-Long, WANG Qing-Wen, JIA Ling-Yun, FENG Han-Qing

College of Life Science, Northwest Normal University, Lanzhou 730070

Received:2017-04-05 Online:2018-01-15 Published:2018-01-06
Supported by:
The National Natural Science Foundation of China(31560070);The Fundamental Research Funds for the Gansu Universities;Gansu Provincial Department of Financeand Gansu University Research Project;Northwest Normal University Innovation Team Project

摘要/Abstract

摘要： 以“农普”12号菜豆幼苗为材料，采用1mmol·L^-1的水杨基氧肟酸（SHAM）抑制交替呼吸途径活性，探讨了在CuCl₂胁迫下交替呼吸途径对菜豆幼苗叶片光系统Ⅱ的保护作用。结果表明，随着CuCl₂胁迫浓度的增加，菜豆幼苗叶片潜在最大光化学效率F_v/F_m、光适应下叶片的最大光化学效率F_v'/F_m'、PSⅡ的实际光化学效率Y（Ⅱ）以及光化学猝灭系数qP、叶绿素含量均呈下降趋势，而非光学猝灭系数NPQ和交替呼吸途径的容量水平则呈上升趋势。较之在CuCl₂处理下的菜豆幼苗，用交替呼吸途径抑制剂预处理后的菜豆再置于CuCl₂的胁迫下，则会导致F_v/F_m、F_v'/F_m'、Y（Ⅱ）、qP以及叶绿素含量的进一步下降和NPQ的进一步上升。上述观察表明，在CuCl₂胁迫下交替呼吸途径可以缓解PSⅡ光化学效率的下降、维持PSⅡ反应中心的开放程度、减少天线色素的热耗散以及缓解叶绿素含量的降低，从而保护菜豆叶片光系统Ⅱ免受CuCl₂胁迫的伤害。

关键词: 交替呼吸途径, CuCl₂胁迫, 菜豆, 叶绿素荧光参数

Abstract: With “Nongpu” No.12 bean seedling leaves(Phaseolus vulgaris L.), we used the AOX inhibitor salicylhydroxamic acid(1 mmol·L^-1; SHAM) to inhibit AOX activity in vivo, and studied the protective effects of alternative respiratory pathway on the photosystem Ⅱ under the CuCl₂ stress. The treatment with CuCl₂ caused a significant decrease in the ratio of F_v/F_m(the potential maximal photochemical efficiency of PSⅡ), F_v'/F_m'(the maximum quantum efficiency of PSⅡ photochemistry at illumination), Y(Ⅱ)(effective photochemical quantum yield of PSⅡ photosynthetic), qP(photochemical quenching coefficient), and the chlorophyll content, but the levels of NPQ(non-photochemical quenching) and the capacity of the alternative respiratory pathway were significantly increased. Compared with the bean seedling under CuCl₂ stress, the CuCl₂-stressed bean seedling pretreated with inhibitor of alternative respiratory(salicylhydroxamic acid) had lower levels of F_v/F_m, F_v'/F_m', Y(Ⅱ), qP and chlorophyll content, and higher levels of NPQ. These observations suggest that under the CuCl₂ stress, the alternative respiratory pathway can protect the photosystem Ⅱ of the bean leaves from CuCl₂ stress by alleviating the decrease of the photochemical efficiency of PSⅡ, maintaining the proportion of open PSⅡ centres, reducing the heat dissipation of the antenna pigment and chlorophyll content disruption.

Key words: alternative respiratory pathway, CuCl₂ stress, Phaseolus vulgaris L., chlorophyll fluorescence

中图分类号:

Q945.79

谢佳佳, 芦丽娜, 石岱龙, 王庆文, 贾凌云, 冯汉青. 交替呼吸途径对CuCl₂胁迫下菜豆叶片光系统Ⅱ的保护作用[J]. 植物研究, 2018, 38(1): 75-80.

XIE Jia-Jia, LU Li-Na, SHI Dai-Long, WANG Qing-Wen, JIA Ling-Yun, FENG Han-Qing. Protective Effects of Alternative Respiratory Pathway on Photosystem Ⅱ in Phaseolus vulgaris under CuCl₂ Stress[J]. Bulletin of Botanical Research, 2018, 38(1): 75-80.

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交替呼吸途径对CuCl₂胁迫下菜豆叶片光系统Ⅱ的保护作用

Protective Effects of Alternative Respiratory Pathway on Photosystem Ⅱ in Phaseolus vulgaris under CuCl₂ Stress

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