雪松松针挥发油化学成分及抗氧化活性研究

doi:10.7525/j.issn.1673-5102.2017.04.019

植物研究 ›› 2017, Vol. 37 ›› Issue (4): 621-627.doi: 10.7525/j.issn.1673-5102.2017.04.019

雪松松针挥发油化学成分及抗氧化活性研究

胡文杰¹, 罗辉², 邹林海¹, 李冠喜³

1. 井冈山大学生命科学学院, 吉安 343009;
2. 井冈山大学医学院, 吉安 343000;
3. 曲阜师范大学生命科学学院, 曲阜 273165

收稿日期:2017-02-21 出版日期:2017-07-15 发布日期:2017-07-22
通讯作者: 李冠喜,E-mail:1040411985@qq.com E-mail:1040411985@qq.com
作者简介:胡文杰(1974-),男,博士,主要从事天然产物化学研究。
基金资助:
江苏省自然基金（BK20151278）；江苏省苏北科技项目（BN2014029）

Chemical Components and Antioxidant Activity of Volatile Oil from Pine Needles of Cedrus deodara

HU Wen-Jie¹, LUO Hui², ZOU Lin-Hai¹, LI Guan-Xi³

1. College of Life Sciences, Jinggangshan University, Ji'an 343009;
2. School of Medicine, Jinggangshan University, Ji'an 343000;
3. School of Life Sciences, Qufu Normal University, Qufu 273165

Received:2017-02-21 Online:2017-07-15 Published:2017-07-22
Supported by:
The Natural Science Foundation of Jiangsu Province(BK20151278);Science and technology projects of northern jiangsu in Jiangsu province(BN2014029)

摘要/Abstract

摘要： 采用水蒸气蒸馏法提取雪松松针挥发油，利用气相色谱-质谱联用技术分析其化学成分。使用维生素C为阳性对照，以DPPH自由基、ABTS自由基和羟基自由基清除作用为指标评价挥发油的抗氧化活性。从雪松松针挥发油中鉴定了37种化学成分，占挥发油总量的95.10%，以烃类和醇类为主；挥发油对DPPH自由基、ABTS自由基和羟基自由基有明显的清除作用，其IC₅₀分别为0.30、0.22及0.96 μg·mL^-1，且呈现一定的量效关系。可见，雪松松针挥发油中含有多种有应用价值的化学成分，具有显著的抗氧化活性。

关键词: 雪松, 挥发油, 化学成分, 气相色谱-质谱联用仪, 抗氧化活性

Abstract: The volatile oil from pine needles of Cedrus deodara was extracted by water-steam distillation and analyzed by GC-MS. The antioxidant activity of volatile oil was evaluated by DPPH·, ABTS· and ·OH scavenging capacity with V_C as the positive control. Thirty-seven components, accounting for 95.10% of total components, were identified. Olefin hydrocarbon and alcohols were found to be dominant components in the volatile oil. The volatile oil exhibited significant scavenging capacity against DPPH·, ABTS· and ·OH in a concentration fashion. When the scavenging rate against DPPH·, ABTS· and ·OH of volatile oil was 50%, the contents of volatile were 0.30, 0.22 and 0.96 μg·mL^-1, respectively. The volatile oil from pine needles of C.deodara contains a variety of chemical composition of application value with a certain antioxidant activity.

Key words: Cedrus deodara, volatile oil, chemical components, GC-MS, antioxidant activity

中图分类号:

胡文杰, 罗辉, 邹林海, 李冠喜. 雪松松针挥发油化学成分及抗氧化活性研究[J]. 植物研究, 2017, 37(4): 621-627.

HU Wen-Jie, LUO Hui, ZOU Lin-Hai, LI Guan-Xi. Chemical Components and Antioxidant Activity of Volatile Oil from Pine Needles of Cedrus deodara[J]. Bulletin of Botanical Research, 2017, 37(4): 621-627.

参考文献

1. 中国科学院中国植物志编辑委员会. 中国植物志:7卷[M]. 北京:科学出版社,1978:200-204.China Flora Editorial Board of CAS. Flora Reipublicae Popularis Sinicae,Tomus 7[M]. Beijing:Science Press,1978:200-204.
2. 刘东彦,石晓峰,李冲,等. 雪松松针黄酮类化学成分的研究[J]. 中草药,2011,42(4):631-633.Liu D Y,Shi X F,Li C,et al. Chemical constituents of flavonoids in pine needles of Cedrus deodara[J]. Chinese Traditional and Herbal Drugs,2011,42(4):631-633.
3. 石晓峰,白朝辉,刘东彦,等. 雪松松针二氯甲烷提取物的化学成分研究[J]. 中药材,2012,35(3):404-406.Shi X F,Bai Z H,Liu D Y,et al. Study on the chemical constituent from the dichloromethane extract of the pine needles of Cedrus deodara[J]. Journal of Chinese Medicinal Materials,2012,35(3):404-406.
4. 郝晓雯,张龙,杜莉谢,等. XRF法测定雪松松针及花粉中的无机元素[J]. 烟台大学学报:自然科学与工程版,2016,29(1):22-27.Hao X W,Zhang L,Du L X,et al. Determination of inorganic elements in pine needles and pine pollen of Cedrus deodara by XRF[J]. Journal of Yantai University:Natural Science and Engineering Edition,2016,29(1):22-27.
5. Chaudhary A K,Ahmad S,Mazumder A. Cognitive enhancement in aged mice after chronic administration of Cedrus deodara Loud. and Pinus roxburghii Sarg. with demonstrated antioxidant properties[J]. Journal of Natural Medicines,2014,68(2):274-283.
6. Dhayabaran N D,Florance E J,Nandakumar R K,et al. Anticonvulsant activity of fraction isolated from ethanolic extract of heartwood of Cedrus deodara[J]. Journal of Natural Medicines,2014,68(2):310-315.
7. Majid A,Azim A,Hussain W,et al. Antibacterial effects of Cedrus deodara oil against pathogenic bacterial strains in vitro approaches[J]. International Journal of Biosciences,2015,6(1):185-191.
8. 李晓凤,焦慧,袁艺,等. 雪松枝叶挥发性物质的化感作用及其化学成分分析[J]. 生态环境学报,2015,24(2):263-269.Li X F,Jiao H,Yuan Y,et al. Allelopathic effects and chemincal components of the volatiles from Cedrus deodara leaves and branches[J]. Ecology and Environmental Sciences,2015,24(2):263-269.
9. Bae G S,Park K C,Choi S B,et al. Protective effects of alpha-pinene in mice with cerulein-induced acute pancreatitis[J]. Life Sciences,2012,91(17-18):866-871.
10. Dorman H J D,Figueiredo A C,Barroso J G,et al. In vitro evaluation of antioxidant activity of essential oils and their components[J]. Flavour and Fragrance Journal,2000,15(1):12-16.
11. Wang W,Li N,Luo M,et al. Antibacterial activity and anticancer activity of Rosmarinus officinalis L. essential oil compared to that of its main components[J]. Molecules,2012,17(3):2704-2713.
12. Aydin E,Türkez H,Geyikoglu F. Antioxidative,anticancer and genotoxic properties of α-pinene on N₂a neuroblastoma cells[J]. Biologia,2013,68(5):1004-1009.
13. Wang W,Wu N,Zu Y G,et al. Antioxidative activity of Rosmarinus officinalis L. essential oil compared to its main components[J]. Food Chemistry,2008,108(3):1019-1022.
14. Liao S L,Shang S B,Shen M G,et al. One-pot synthesis and antimicrobial evaluation of novel 3-cyanopyridine derivatives of(-)-β-pinene[J]. Bioorganic & Medicinal Chemistry Letters,2016,26(6):1512-1515.
15. Li J,Tian X R,Gao Y Q,et al. A value-added use of volatile turpentine:antifungal activity and QSAR study of β-pinene derivatives against three agricultural fungi[J]. RSC Advances,2015,5(82):66947-66955.
16. Moreira Í J,Menezes P P,Serafini M R,et al. Characterization and antihypertensive effect of the complex of(-)-β-pinene in β-cyclodextrin[J]. Current Pharmaceutical Biotechnology,2016,17(9):837-845.
17. Guzmán-gutiérrez S L,Gómez-cansino R,García-zebadúa J C,et al. Antidepressant activity of Litsea glaucescens essential oil:identification of β-pinene and linalool as active principles[J]. Journal of Ethnopharmacology,2012,143(2):673-679.
18. 王金娥,朱岳麟,熊常健. 月桂烯的来源及其在香料化学中的应用[J]. 山东化工,2011,40(3):47-50.Wang J E,Zhu Y L,Xiong C J. Sources and application in perfume chemistry of myrcen[J]. Shandong Chemical Industry,2011,40(3):47-50.
19. Sun J. D-Limonene:safety and clinical application[J]. Alternative Medicine Review,2007,12(3):259-264.
20. Whysner J,Williams G M. D-Limonene mechanistic data and risk assessment:absolute species-specific cytotoxicity,enhanced cell proliferation,and tumor promotion[J]. Pharmacology & Therapeutics,1996,71(1-2):127-136.
21. Bacanli M,Başaran A A,Başaran N. The antioxidant and antigenotoxic properties of citrus phenolics limonene and naringin[J]. Food and Chemical Toxicology,2015,81:160-170.
22. 葛丽娜,韩雪,任珂珂,等. 火棘花挥发油化学成分的GC-MS分析及抗氧化活性研究[J]. 植物研究,2014,34(2):276-281.Ge L N,Han X,Ren K K,et al. GC-MS analysis on chemical constituents and antioxidant activity of volatile oil from pyracantha fortuneana flowers[J]. Bulletin of Botanical Research,2014,34(2):276-281.
23. 陈志伟,程鹏,王如刚,等. 石榴花挥发油化学成分的GC-MS分析及体外抗氧化活性测定[J]. 中国医院药学杂志,2013,33(4):280-282.Chen Z W,Cheng P,Wang R G,et al. Analysis of the chemical constituents of essential oil from pomegranate flower by GC-MS and determination of its antioxidant activity in vitro[J]. Chinese Journal of Hospital Pharmacy,2013,33(4):280-282.

[1]	侯雪月, 邓俊杰, 姚志红, 黄寿臣, 李嘉哲, 茶新有, 马德志, 张荣沭. 木霉菌对芸芥生长相关生理指标的影响[J]. 植物研究, 2020, 40(3): 347-352.
[2]	张力凡, 薛煜, 王岩, 杨光鑫, 杨光, 邸雪颖. 樟子松乙醇提取物体外抗氧化活性研究[J]. 植物研究, 2019, 39(3): 458-465.
[3]	胡文杰, 许樟润, 鲁思情, 刘思敏, 曾建军. 皇菊花发育过程中挥发油化学成分及其抗氧化活性的研究[J]. 植物研究, 2016, 36(6): 942-948.
[4]	潘苗, 左菲菲, 盛继露, 常强强, 范佳佳, 张小平. 南方红豆杉不同种源种子千粒重、生活力、营养化学成分含量的比较及其与生态因子的关系[J]. 植物研究, 2016, 36(3): 360-367.
[5]	罗猛1,2；宋卓悦1,2；胡娇阳1,2；牟璠松1,2*；乔琪1,2；祖元刚1,2. 超声法提取山里红叶总黄酮及其抗氧化活性研究[J]. 植物研究, 2015, 35(4): 632-637.
[6]	葛丽娜;韩雪;任珂珂;章甫;彭永宇;毕淑峰*. 火棘花挥发油化学成分的GC-MS分析及抗氧化活性研究[J]. 植物研究, 2014, 34(2): 276-281.
[7]	王冬梅;吕振江;王永红;张辉辉. 不同炮制方法对玉竹提取物得率及体外抗氧化作用的影响[J]. 植物研究, 2012, 32(5): 621-626.
[8]	高翔;武佳业;张直峰;闫桂琴*. 翅果油树不同部位多糖含量及抗氧化活性比较研究[J]. 植物研究, 2011, 31(3): 363-366.
[9]	祖元刚;苏鹭;赵修华;王化;张琳. 干姜挥发油纳米乳的制备与表征[J]. 植物研究, 2010, 30(5): 637-640.
[10]	洪阁;刘培勋;高小荣;沈秀;徐文清. 银耳碱提多糖的纯化、化学表征及其抗氧化作用的研究[J]. 植物研究, 2010, 30(2): 221-227.
[11]	范现丽;王高;王宏;范建芝;申晓辉*. 香桃木叶片粗多酚抗氧化活性的研究[J]. 植物研究, 2009, 29(3): 375-379.
[12]	朱岳麟;郑晓梅;王文广. 早熟金柚果皮挥发油的提取及GC-MS分析[J]. 植物研究, 2009, 29(2): 253-256.
[13]	于景华;李艳华;张学科;祖元刚*;唐中华;常　影;高银祥. 转UGPase基因喜树木材化学成分与生长速率研究[J]. , 2007, 27(5): 612-615.
[14]	尚　洁;贾洪柏;王秋玉*. 白桦天然种群木材化学成分的地理变异与相关性研究[J]. 植物研究, 2007, 27(5): 607-611.
[15]	赵子峰;沙伟*;金忠民;谭大海. 杜香挥发油的超临界CO₂萃取实验条件优化[J]. 植物研究, 2006, 26(3): 377-379.

雪松松针挥发油化学成分及抗氧化活性研究

Chemical Components and Antioxidant Activity of Volatile Oil from Pine Needles of Cedrus deodara

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价