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植物研究 ›› 2017, Vol. 37 ›› Issue (4): 587-595.doi: 10.7525/j.issn.1673-5102.2017.04.015

• 论文 • 上一篇    下一篇

甘薯MADS-box转录因子IbMADS11-Like的克隆及胁迫响应分析

谈传婷, 宋炜涵, 朱明库, 徐涛, 董婷婷, 李宗芸   

  1. 江苏师范大学生命科学学院, 徐州 221116
  • 收稿日期:2016-12-20 出版日期:2017-07-15 发布日期:2017-07-22
  • 通讯作者: 董婷婷,E-mail:dtt@jsnu.edu.cn E-mail:dtt@jsnu.edu.cn
  • 作者简介:谈传婷(1996-),女,本科生,主要从事植物分子生物学方面的研究工作。
  • 基金资助:
    国家自然科学基金(31501352);江苏省自然科学基金(BK20150229);大学生实践创新训练计划项目(201510320022,201510320022Z);江苏师范大学博士学位教师科研支持项目(14XLR012)

Cloning and Expression Analysis of Stress Treatments of a MADS-box Transcription Factor in Sweet Potato

TAN Chuan-Ting, SONG Wei-Han, ZHU Ming-Ku, XU Tao, DONG Ting-Ting, LI Zong-Yun   

  1. School of Life Science, Jiangsu Normal University, Xuzhou 221116
  • Received:2016-12-20 Online:2017-07-15 Published:2017-07-22
  • Supported by:
    Supported by the National Natural Science Foundation of China(31501352);Natural Science Foundation of Jiangsu Provence(BK20150229);National Training Programs of Innovation and Entrepreneurship for Undergraduate(201510320022,201510320022Z);Jiangsu Normal University doctoral degree teachers research support project(14XLR012)

摘要: MADS-box蛋白在植物生长发育及抗逆等过程中均发挥重要功能。本实验室根据甘薯近缘野生种I.trifida基因组序列,在甘薯栽培种徐薯22(Ipomoea batatas(L.) Xu22)中克隆到一个STMADS11亚家族MADS-box基因,命名为IbMADS11-Like。实时定量RT-PCR分析表明,IbMADS11-Like基因在甘薯根中大量表达,并且随着块根的形成和膨大表达量逐渐降低,表明该基因可能参与了甘薯块根的发育过程。胁迫处理分析表明,IbMADS11-Like基因的表达受干旱、盐和高温的诱导,而低温则抑制其表达。此外,IbMADS11-Like基因对ABA、IAA、ZT、BR、ACC、JA及GA等激素的处理也有不同程度的响应,暗示IbMADS11-Like基因可能参与了甘薯生长发育及胁迫的调控过程。这些结果为进一步分析IbMADS11-Like基因在甘薯块根发育和胁迫响应中的功能奠定了基础。

关键词: MADS-box蛋白, IbMADS11-Like, 转录因子, 克隆, 非生物胁迫, 甘薯

Abstract: MADS-box proteins play important functions in the development and stress response of plants. A STMADS11 subfamily gene was cloned from Ipomoea batatas(L.) Xu22 according to I.trifida genome sequences, named IbMADS11-Like. By real-time RT-PCR IbMADS11-Like was expressed abundantly in roots, and a rapid declining trend was observed as storage root formation and development. The IbMADS11-Like might be involved in the storage root development of sweet potato. Stress treatment experiments showed that IbMADS11-Like was induced by drought, NaCl and high temperature, while was reduced by low temperature. ABA, IAA, ZT, BR, ACC, JA and GA treatments also impacted the expression of IbMADS11-Like, indicating that IbMADS11-Like might play functions in the development and stress response in sweet potato. These results play a foundation for further study on the physiological function of IbMADS11-Like in the development and stress response of sweet potato.

Key words: MADS-box protein, IbMADS11-Like, transcription factor, cloning, abiotic stress, sweet potato

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