基于rbcL序列试论鸢尾属部分物种间的系统发育关系

doi:10.7525/j.issn.1673-5102.2017.03.005

摘要/Abstract

摘要： 选用叶绿体rbcL基因对鸢尾属植物的分类和系统发育进行了分析。以雄黄兰为外类群，对21份鸢尾属材料和GenBank下载的共49条序列采用最大简约法构建系统进化树，探讨了鸢尾属的分类系统。针对有争议的物种，聚类结果表明：马蔺与白花马蔺关系较远；扁竹兰和蝴蝶花亲缘关系较扇形鸢尾近；细锐果鸢尾和锐果鸢尾亲缘关系近，大锐果鸢尾较之远；四川鸢尾和薄叶鸢尾聚类近；西伯利亚鸢尾系内物种间聚类复杂，亲缘关系不清楚。

关键词: 鸢尾属, rbcL序列, 系统发育

Abstract: The phylogenesis of Iris L. was analyzed using the chloroplast genes rbcL. Classification was performed with maximum parsimony method and the gene tree were constructed with Crocosmia crocosmiflora as outgroup, 21 wild populations of Iris L. from China and download 49 sequences from GenBank. As for disputed species, clustering trees results showed Iris lactea var. chinensis(Fisch.) Koidz. had close relationship with Iris lactea Pall.. Iris confusa Sealy had more close relationship with Iris japonica Thunb. than that with Iris wattii Baker. Iris goniocarpa var. tenella Y.T. Zhao was very relevant to Iris goniocarpa Baker, but Iris goniocarpa var. grossa Y.T. Zhao was distant. Iris sichuanensis Y.T. Zhao and Iris leptophylla Lingelsh. were closely related. Clustering relationships of Series Sibirica were very complexed, and genetic relationgships were not clear.

Key words: Iris L., rbcL sequence, systematic classification

中图分类号:

Q949.71+8.28

蒋喻林, 刘宇婧, 冯艺玫, 谭淼, 蒋天仪, 余小芳. 基于rbcL序列试论鸢尾属部分物种间的系统发育关系[J]. 植物研究, 2017, 37(3): 351-359.

JIANG Yu-Lin, LIU Yu-Jing, FENG Yi-Mei, TAN Miao, JIANG Tian-Yi, YU Xiao-Fang. Phylogenetic Relationship of Several Species from Iris L. Based on rbcL Sequences[J]. Bulletin of Botanical Research, 2017, 37(3): 351-359.

参考文献

1. Guo J Y,Wilson C A. Molecular phylogeny of crested Iris based on five plastid markers(Iridaceae)[J]. Systematic Botany,2013,38(4):987-995.
2. Raven P H,Axelrod D I. Angiosperm biogeography and past continental movements[J]. Annals of the Missouri Botanical Garden,1974,61(3):539-673.
3. 赵毓棠. 中国植物志[M]. 北京:科学出版社,1985:120-198.Zhao Y T. Flora of China[M]. Beijing:Science Press,1985:120-198.
4. Zhao Y T,Noltie H J,Mathew B. Iridaceae[M]. //Wu Z Y,Raven P H. Flora of China. Beijing,China,St. Louis,Missouri,USA:Science Press,Missouri Botanical Garden Press,2000:297-313.
5. Wilson C A. A Cladistic analysis of Iris series Californicae based on morphological data[J]. Systematic Botany,1998,23(1):73-88.
6. 齐耀斌,赵毓棠. 中国鸢尾属花粉形态研究[J]. 植物分类学报,1987,25(6):430-436.Qi Y B,Zhao Y T. Studies on the pollen morphology of the genus Iris in China[J]. Journal of Systematics and Evolution,1987,25(6):430-436.
7. 余小芳,张海琴,何雪梅,等. 鸢尾属12种(变种)植物花粉形态及其系统学意义[J]. 园艺学报,2010,37(7):1175-1182.Yu X F,Zhang H Q,He X M,et al. Pollen morphology of 12 species of Iris L. and it's systematic significations[J]. Acta Horticulturae Sinica,2010,37(7):1175-1182.
8. Mitió B,Halbritter H,Šoštaric R,et al. Pollen morphology of the genus Iris L. (Iridaceae) from Croatia and surrounding area:taxonomic and phylogenetic implications[J]. Plant Systematics and Evolution,2013,299(1):271-288.
9. 周永红,伍碧华,颜济,等. Iris japonica×Iris confusa种间杂种的细胞遗传学研究[J]. 云南植物研究,2003,25(4):497-502.Zhou Y H,Wu B H,Yan J,et al. Cytogenetic study on the interspecific hybrid between Iris japonica and Iris confusa(Irisaceae)[J]. Acta Botanica Yunnanica,2003,25(4):497-502.
10. Ikinci N,Hall T,Lledó M D,et al. Molecular phylogenetics of the Juno Irises,Iris subgenus Scorpiris(Iridaceae),based on six plastid markers[J]. Botanical Journal of the Linnean Society,2011,167(3):281-300.
11. 沈云光,王仲朗,管开云. 国产13种鸢尾属植物的核型研究[J]. 植物分类学报,2007,45(5):601-618.Shen Y G,Wang Z L,Guan K Y. Karyotypical studies on thirteen Iris plants from China[J]. Acta Phytotaxonomica Sinica,2007,45(5):601-618.
12. Wilson C A. Phylogenetic relationships in Iris series Californicae based on ITS sequences of nuclear ribosomal DNA[J]. Systematic Botany,2003,28(1):39-46.
13. Wheeler A S,Wilson C A. Exploring phylogenetic relationships within a broadly distributed northern hemisphere group of semi-aquatic Iris species(Iridaceae)[J]. Systematic Botany,2014,39(3):759-766.
14. 牟少华,彭镇华,郄光发,等. 部分鸢尾属植物的AFLP标记[J]. 东北林业大学学报,2011,39(1):124-126.Mu S H,Peng Z H,Qie G F,et al. Genetic diversity of Iris determined by AFLP marker[J]. Journal of Northeast Forestry University,2011,39(1):124-126.
15. 张敏,黄苏珍. 鸢尾属部分植物种质资源的RAPD分析[J]. 西北植物学报,2008,28(5):933-939.Zhang M,Huang S Z. RAPD analysis on some germplasm of Iris L. plants[J]. Acta Botanica Boreali-Occidentalia Sinica,2008,28(5):933-939.
16. 张敏,黄苏珍,仇硕,等. 鸢尾属植物遗传多样性的RAPD和ISSR分析[J]. 植物资源与环境学报,2007,16(2):6-11.Zhang M,Huang S Z,Qiu S,et al. Analysis of RAPD and ISSR on genetic diversity of Iris L. plants[J]. Journal of Plant Resources and Environment,2007,16(2):6-11.
17. Wilson C A. Phylogeny of Iris based on chloroplast matK Gene and trnK intron sequence data[J]. Molecular Phylogenetics and Evolution,2004,33(2):402-412.
18. 高宝莼,谭仲明. 四川植物志:第9卷[M]. 成都:四川民族出版社,1989:320-370.Gao B C,Tan Z M. Flora Sichuanica:vol. 9[M]. Chengdu:Sichuan National Publishing House,1989:320-370.
19. 高宝莼. 四川鸢尾属的研究[J]. 西南师范学院学报,1985(3):11-15.Gao B C. Notes on the genus Iris of Sichuan[J]. Journal of the Southeast Teachers Collage,1985(3):11-15.
20. 余小芳. 四川鸢尾属植物的系统学及种子休眠与萌发特性研究[D]. 雅安:四川农业大学,2009.Yu X F. Systematic study and seed dormancy and germination characters of Iris spieces in Sichuan[D]. Ya'an:Sichuan Agricultural University,2009.
21. 仲轶. 基于多基因组的鸢尾属部分种的系统分类研究[D]. 哈尔滨:东北林业大学,2010.Zhong Y. The system classification research base on multi-genome of some Iris species[D]. Harbin:Northeast Forestry University,2010.
22. Colasante M,Sauer W. The genus Iris L. in Latium(central Italy):general remarks on the morphological and karyological differentiation as well as on the ecological adaptation and flowering periods[J]. Linzer Biologische Beiträge,1993,25(2):1189-1204.
23. 舒焕麟,颜济,杨俊良. Iris confusa Sealy的细胞学研究[J]. 四川农业大学学报,1992,10(4):586-591.Shu H L,Yan J,Yang J L. Cytological study on Iris confusa Sealy[J]. Journal of Sichuan Agricultural University,1992,10(4):586-591.
24. 毛节绮,薛详骥. 浙江几种鸢尾科植物的染色体[J]. 浙江农业大学学报,1986,12(1):97-101.Mao J Q,Xue X J. Chromosome numbers of thirteen iridaceous species from Zhejiang province[J]. Journal of Zhejiang Agricultural University,1986,12(1):97-101.
25. Wilson C A. Phylogenetic relationships among the recognized series in Iris section Limniris[J]. Systematic Botany,2009,34(2):277-284.
26. Makarevitch I,Golovnina K,Scherbik S,et al. Phylogenetic relationships of the Siberian Iris species inferred from noncoding chloroplast DNA sequences[J]. International Journal of Plant Sciences,2003,164(2):229-237.
27. Chase M W,Soltis D E,Olmstead R G,et al. Palmer,phylogenetics of seed plants:an analysis of nucleotide sequences from the plastid gene rbcL[J]. Annals of the Missouri Botanical Garden,1993,80(3):528-580.
28. Soltis D E,Soltis P S,Chase M W,et al. Angiosperm phylogeny inferred from 18S rDNA,rbcL,and atpB sequences[J]. Botanical Journal of the Linnean Society,2000,133(4):381-461.
29. Donoghue M J,Olmstead R G,Smith J F,et al. Phylogenetic relationships of Dipsacales based on rbcL sequences[J]. Annals of the Missouri Botanical Garden,1992,79(2):333-345.
30. Chase M W,Bruijn A Y D,Cox A V,et al. Phylogenetics of asphodelaceae(asparagales):an analysis of plastid rbcL and trnL-F DNA sequences[J]. Annals of Botany,2000,86(5):935-951.
31. Reeves G,Chase M W,Goldblatt P,et al. Molecular systematics of iridaceae:evidence from four plastid DNA regions[J]. American Journal of Botany,2001,88(11):2074-2087.
32. Thompson J D,Higgins D G,Gibson T J. CLUSTAL W:improving the sensitivity of progressive multiple sequence alignment through sequence weighting,position-specific gap penalties and weight matrix choice[J]. Nucleic Acids Research,1994,22(22):4673-4680.
33. Xia X H. DAMBE5:a comprehensive software package for data analysis in molecular biology and evolution[J]. Molecular Biology and Evolution,2013,30(7):1720-1728.
34. Tamura K,Stecher G,Peterson D,et al. MEGA6:molecular evolutionary genetics analysis version 6.0[J]. Molecular Biology and Evolution,2013,30(12):2725-2729.
35. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences[J]. Journal of Molecular Evolution,1980,16(2):111-120.
36. Tajima F. Statistical methods to test for nucleotide mutation hypothesis by DNA polymorphism[J]. Genetics,1989,123:585-595.
37. 谢航. 中国鸢尾属有关分类群的讨论及属下分类系统的修订[D]. 长春:东北师范大学,1996.Xie H. Discussion of some taxa and revision of the classification system of Chinese Iris[D]. Changchun:Northeast Normal University,1996.
38. 王玲,卓丽环. 鸢尾属部分种种子微形态特征与系统演化关系[J]. 植物研究,2006,26(3):286-290.Wang L,Zhuo L H. The relationship between seed coat micro-morphology characteristics and systemic evolution of some species of Iris[J]. Bulletin of Botanical Research,2006,26(3):286-290.
39. 王玲,卓丽环. 基于ITS序列的鸢尾属植物部分种的系统分类[J]. 东北林业大学学报,2006,34(4):54-56,75.Wang L,Zhuo L H. Systematic classification of some species of Iris based on ITS sequences[J]. Journal of Northeast Forestry University,2006,34(4):54-56,75.
40. Wilson C A. Subgeneric classification in Iris re-examined using chloroplast sequence data[J]. Taxon,2011,60(1):27-35.
41. Rodionenko G I. The genus Iris L. [M]. London:The British Iris Society,1987.
42. Mathew B. The Iris[M]. London:B. T. Batsford,1989.
43. Wilson C A,Calvin C L. An origin of aerial branch parasitism in the mistletoe family,Loranthaceae[J]. American Journal of Botany,2006,93(5):787-796.
44. 牟少华,孙振元,彭镇华. 基于叶绿体DNA trnL-F序列研究部分鸢尾属的亲缘关系[J]. 华北农学报,2010,25(5):112-116.Mu S H,Sun Z Y,Peng Z H. Relationship of Iris inferred from the chloroplast DNA trnL-F sequence variation[J]. Acta Agriculturae Boreali-Sinica,2010,25(5):112-116.
45. Goldblatt P,Manning J C. The Iris family:natural history and classification[M]. Portland:Timber Press,2008.
46. 牟少华,彭镇华,郄光发,等. 马蔺种质资源AFLP标记遗传多样性分析[J]. 安徽农业大学学报,2008,35(1):95-98.Mu S H,Peng Z H,Qie G F,et al. Genetic diversity of Iris lactea determined by AFLP marker[J]. Journal of Anhui Agricultural University,2008,35(1):95-98.
47. Waddick J W. Collecting Irises in western China[J]. Bulletin of the American Iris Society,1994(3):74-92.

[1]	万雪琴, 时羽杰, 黄金亮, 糜加轩, 钟宇, 张帆, 陈良华. 杨属植物分类的研究进展和展望[J]. 植物研究, 2023, 43(2): 161-168.
[2]	王飞, 赵文植, 董章宏, 马路遥, 李卫英, 李宗艳, 辛培尧. 丝兰属6种植物叶绿体基因组特征分析[J]. 植物研究, 2023, 43(1): 109-119.
[3]	芦永昌, 张鑫, 张璐燕, 王久利. 辽东丁香完整叶绿体基因组的结构与特征[J]. 植物研究, 2023, 43(1): 120-130.
[4]	张雨, 苏旭, 刘玉萍, 刘涛, 郑长远, 苏丹丹, 王亚男, 吕婷. 喜马红景天叶绿体基因组特征及其系统发育分析[J]. 植物研究, 2022, 42(4): 602-612.
[5]	夏铭泽, 张发起, 迟晓峰, 韩霜, 陈世龙. 梅花草属叶绿体基因组进化分析[J]. 植物研究, 2022, 42(4): 626-636.
[6]	苏丹丹, 刘玉萍, 刘涛, 郑长远, 张雨, 王亚男, 秦娜, 苏旭. 苦马豆叶绿体基因组结构及其特征分析[J]. 植物研究, 2022, 42(3): 446-454.
[7]	曹倩, 徐隆华, 王久利, 张发起, 陈世龙. 獐牙菜亚族植物的系统发育分析[J]. 植物研究, 2021, 41(3): 408-418.
[8]	杨晨, 陈志祥, 姚雪莹, 王河山, 王奇志. 中国15种变豆菜属植物的花粉形态及系统学分析[J]. 植物研究, 2020, 40(6): 805-812.
[9]	付涛, 王志龙, 林乐静, 林立, 李文, 袁冬明. 中国主栽樱花的分子系统发育分析[J]. 植物研究, 2020, 40(6): 876-885.
[10]	张衷华, 宋晓倩, 唐中华, 梁正伟. 中国盐生植物系统发育多样性及省域差异性[J]. 植物研究, 2020, 40(3): 330-338.
[11]	段义忠, 杜忠毓, 王海涛. 西北干旱区孑遗濒危植物四合木(Tetraena mongolica)叶绿体基因组特征研究及比较分析[J]. 植物研究, 2019, 39(5): 653-663.
[12]	朱弘, 伊贤贵, 朱淑霞, 王华辰, 段一凡, 王贤荣. 基于叶绿体DNA atpB-rbcL片段的典型樱亚属部分种的亲缘关系及分类地位探讨[J]. 植物研究, 2018, 38(6): 820-827.
[13]	张妍彤, 黄剑, 宋菊, 林丽梅, 冯若宣, 邢朝斌. 壳斗科植物叶绿体基因组结构及变异分析[J]. 植物研究, 2018, 38(5): 757-765.
[14]	唐丽丽, 陈国平, 冯小梅, 赵铁建, 石福臣. 基于系统发育的燕山东麓植物群落的构建机制[J]. 植物研究, 2017, 37(6): 807-815.
[15]	南芳茹;冯佳;谢树莲*. 中国熊野藻属植物系统发育分析—基于叶绿体UPA序列[J]. 植物研究, 2014, 34(5): 584-591.