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植物研究 ›› 2011, Vol. 31 ›› Issue (3): 313-317.doi: 10.7525/j.issn.1673-5102.2011.03.011

• 论文 • 上一篇    下一篇

AtAAPT1基因RNAi的植物表达载体的构建

郑桂灵;李鹏   

  1. 西南科技大学生命科学与工程学院,绵阳 621010
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-05-20 发布日期:2011-05-20
  • 基金资助:
     

Construction of Plant Transformation Vector of AtAAPT1 Gene for RNAi

ZHENG Gui-Ling;LI Peng   

  1. School of Life Sciences and Engineering,Southwest University of Science and Technology,Mianyang 621000
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-05-20 Published:2011-05-20
  • Supported by:
     

摘要: 以拟南芥中氨基乙醇磷酸转移酶基因AAPT1作为RNAi的靶向序列,采用RT-PCR的方法获得目的DNA片段。然后以pBS-T-AAPT1载体为基础,构建了由35s启动子调控的AAPT1基因RNAi的植物表达载体pART27-AAPT1(1,2),并通过电击法将重组质粒导入根癌农杆菌C58中。AtAAPT1基因RNAi的植物表达载体的构建对于研究AAPT1基因的功能和应用具有重要的理论价值。

关键词: AAPT1基因, RNAi, 植物表达载体

Abstract: Aminoalcoholphosphotransferase gene AAPT1 from Arabidopsis thaliana was used as target sequence for RNA interference in this paper. The target sequence was linked to pBS-T cloning vector after obtained by RT-PCR reaction. A plant transformation vector pART27-AAPT1(1,2) was constructed from pBS-T, including AAPT1 gene controlled by CaMV35s promoter in this experiment. Then the recombination plasmid was transfected into Agrobacterium C58 by electroporation-mediated way.

Key words: AAPT1 gene, RNAi, plant transformation vector

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