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植物研究 ›› 2017, Vol. 37 ›› Issue (1): 147-154.doi: 10.7525/j.issn.1673-5102.2017.01.019

• 论文 • 上一篇    下一篇

植物细胞壁组织化学定位染色方法和技术的比较研究

张霞, 胡露洁, 周存宇, 杨朝东   

  1. 长江大学园艺园林学院, 荆州 434025
  • 收稿日期:2016-07-28 出版日期:2017-01-15 发布日期:2017-03-06
  • 通讯作者: 杨朝东,E-mail:chaodongyang@aliyun.com E-mail:chaodongyang@aliyun.com
  • 作者简介:张霞(1981-),女,硕士,主要从事植物学研究。
  • 基金资助:
    湿地生态与农业利用教育部工程研究中心开放基金(KF201603)

Comparative Study on Staining Methods and Techniques of Cell Wall Histochemistry

ZHANG Xia, HU Lu-Jie, ZHOU Cun-Yu, YANG Chao-Dong   

  1. College of Gardening and Horticulture, Yangtze University, Jingzhou 434025
  • Received:2016-07-28 Online:2017-01-15 Published:2017-03-06
  • Supported by:
    Engineering Research Center of Ecology and Agriculture Use of Wetland, Ministry of Education opening fund(KF201603)

摘要: 利用光学和荧光显微镜比较研究几种植物细胞壁组织化学定位染色方法和技术,结果表明:(1)硫酸消化法和硫酸氢黄连素-苯胺兰对染法研究凯氏带,对取材时间和部位要求高,建议两种方法配合使用,可相互印证是否具凯氏带;(2)苏丹7B染色法,蓝色激发光下不染色和硫酸氢黄连素-苯胺兰对染研究细胞壁栓质层3种方法中,不染色蓝色激发光下结果比苏丹7B染色法敏感显色,但苏丹7B染色法在普通光学显微镜下观察较为便捷;(3)木质化细胞壁染色方法中硫酸氢黄连素-苯胺兰对染法比间苯三酚-盐酸染色法易显色观察;(4)甲苯胺兰快速染色细胞壁取代常规苏丹Ⅲ/Ⅳ法,细胞边界和层次更清楚。

关键词: 组织化学, 凯氏带, 栓质层, 木质化

Abstract: We carried out the comparative studies on several methods and techniques for cell wall histochemistry under light and epifluorescence microscope. The results showed that:(1) To study Casparian bands using staining methods of sulphuric acid digestion and berberine hemisulfate-aniline, the requirements of material collecting time and part were high, therefore, it was recommended to use the two methods to mutual confirmation; (2) To study suberin lamellae using Sudan red 7B staining, and none staining and berberine hemisulfate-aniline blue staining under blue excited, none staining was more sensitive than Sudan red 7B, but Sudan red 7B staining is convenient under light microscope; (3) To study lignified walls, berberine hemisulfate-aniline staining was more sensitive than phloroglucinol-HCl; (4) Using TBO fast wall staining to replace Sudan Ⅲ/Ⅳ conventional staining, the wall borders and layers were clearer.

Key words: walls histochemistry, Casparian bands, suberin lamellae, lignified walls

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