白桦BpCesA基因的生物信息学及表达分析

doi:10.7525/j.issn.1673-5102.2016.06.015

植物研究 ›› 2016, Vol. 36 ›› Issue (6): 909-916.doi: 10.7525/j.issn.1673-5102.2016.06.015

白桦BpCesA基因的生物信息学及表达分析

申婷婷¹, 姜静¹, 刘桂丰¹, 许思佳¹, 李慧玉¹, 袁红梅^1,2

1. 东北林业大学林木遗传育种国家重点实验室, 哈尔滨 150040;
2. 黑龙江省农业科学院经济作物研究所, 哈尔滨 150086

收稿日期:2016-09-09 出版日期:2016-11-15 发布日期:2016-11-16
通讯作者: 李慧玉,E-mail:lihuiyu0519@aliyun.com E-mail:lihuiyu0519@aliyun.com
作者简介:申婷婷(1990-),女,硕士研究生,主要从事林木遗传育种研究。
基金资助:
“十二五”农村领域国家科技计划课题（2012BAD21B02）；东北林业大学林木遗传育种国家重点实验室开放基金项目（201203）

Bioinformatics and Expression Analysis of BpCesA Genes of Betula platyphylla

SHEN Ting-Ting¹, JIANG Jing¹, LIU Gui-Feng¹, XU Si-Jia¹, LI Hui-Yu¹, YUAN Hong-Mei^1,2

1. State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040;
2. Industrial Crops Institute of Heilongjiang Academy of Agricultural Sciences, Harbin 150086

Received:2016-09-09 Online:2016-11-15 Published:2016-11-16
Supported by:
National Science and Technology Planning Project of "12th Five-Year"(2012BAD21B02); State Key Laboratory of Tree Genetics and Breeding Northeast Forestry University(201203)

摘要/Abstract

摘要： 从白桦45个转录组数据中分析获得6条白桦纤维素合成酶基因，其中2条为该家族的新基因，通过生物信息学及实时定量PCR技术探讨白桦BpCesA在不同材性白桦中的表达量情况。结果表明，6条白桦BpCesA基因的ORF长度在2 958~3 312 bp，编码的氨基酸数目在985~1 103 aa，相对分子量在110.40~124.29 kD，理论等电点为5.90~7.43；6条BpCesA均含有D，D，D，QXXRW保守结构域和8个跨膜螺旋，其中2个位于N端，6个位于C端。对来自6个双子叶植物，4个单子叶植物和1个裸子植物的52条CesA进行了聚类分析发现：白桦BpCesA与同为木本的杨树PtrCesA具有较高的同源性，其中BpCesA2与PtrCesA3同源性达到92%、另外BpCesA5与PtrCesA6、BpCesA1与PtrCesA8也具有较高的同源性，分别为89%和82%。BpCesA1、BpCesA2、BpCesA3、BpCesA5基因随纤维素含量的增加，表达量也呈现增加趋势，推测这些基因在白桦的纤维素合成中起到促进的作用。本研究为揭示纤维素合成酶基因的功能分析提供了基础。

关键词: 白桦, 纤维素, 木质素, 生物信息学, 表达量

Abstract: Six cellulose synthase genes of Betula platyphylla from 45 transcriptome data sets were obtained, in which two genes were new genes of cellulose synthase gene family. The sequence characteristic and expression levels in Birch xylem of BpCesA were analyzed using bioinformatics and quantitative real-time PCR. The ORF length of 6 BpCesA genes varied from 2 958-3 312 bp; Number of amino acids encoded varied from 985-1 103 aa, and relative molecular weight varied from 110.40-124.29 kD; Theoretical isoelectric point was from 5.90-7.43. Six BpCesA genes all contained D, D, D, QXXRW conservative structure domain and eight transmembrane helices, in which two were located at the end of the N, and the others were at the end of the C. 52 CesAs from six dicotyledonous plants, four monocotyledons, and one gymnosperms were analyzed by cluster analysis, and BpCesAs had higher homology with PtrCesA. The homology between BpCesA2 and PtrCesA3 reached 92%. BpCesA5 and PtrCesA6, BpCesA1 and PtrCesA8 also had high homology of 89% and 82%, respectively. The RNA level of BpCesA1, BpCesA2, BpCesA3 and BpCesA5 was increased with the increase of cellulose content. This results suggested that these genes play a positive role in cellulose synthesis in birch. This study provides a basis for the functional analysis of cellulose synthase gene.

Key words: Betula platyphylla, cellulose, lignin, bioinformatics, expression quantity

中图分类号:

S792.153

申婷婷, 姜静, 刘桂丰, 许思佳, 李慧玉, 袁红梅. 白桦BpCesA基因的生物信息学及表达分析[J]. 植物研究, 2016, 36(6): 909-916.

SHEN Ting-Ting, JIANG Jing, LIU Gui-Feng, XU Si-Jia, LI Hui-Yu, YUAN Hong-Mei. Bioinformatics and Expression Analysis of BpCesA Genes of Betula platyphylla[J]. Bulletin of Botanical Research, 2016, 36(6): 909-916.

参考文献

1. 郁书君,汪天,金宗郁,等.白桦容器栽培试验(Ⅰ)-最适基质配方的筛选[J].北京林业大学学报,2001,23(1):24-28. Yu S J,Wang T,Kim J W,et al.Container culture experiments of Betula platyphylla (Ⅰ)Screening of the optimum formula for substrate[J].Journal of Beijing Forestry University,2001,23(1):24-28.
2. Wei Z G,Zhang K X,Yang C P,et al.Genetic linkage maps of Betula platyphylla Suk based on ISSR and AFLP markers[J].Plant Molecular Biology Reporter,2010,28:169.
3. 宋福南,邢磊,陈肃,等.白桦苯丙氨酸解氨酶(PAL)基因的分离及其表达[J].东北林业大学学报,2009,37(12):14-17,47. Song F N,Xing L,Chen S,et al.Expression and functional analysis of correlative enzyme genes of lignin phenylpropanoid biosynthetic pathway in Betula PLATPHYLLA[J].Journal of Northeast Forestry University,2009,37 (12):14-17,47.
4. 刘雪梅,杨传平,耿峰.白桦胚珠发育及胚、胚乳发育关系的研究[J].植物研究,2005,25(3):322-326. Liu X M,Yang C P,Geng F.Development of ovule and relation between embryo and endosperm developments of Betula platyphylla[J].Plant research,2005,25(3):322-326.
5. 陈鹏飞.白桦纤维素合成酶基因克隆与表达特征分析[D].哈尔滨:东北林业大学,2008. Chen P F.Cloning and expression characteristics of birch cellulose synthase gene analysis[D].Harbin:Northeast Forestry University,2008.
6. Arioli T,Peng L C,Betzner A S,et al.Molecular analysis of cellulose biosynthesis in Arabidopsis[J].Science,1998,279(5351):717-720.
7. Gardiner J C,Taylor N G,Turner S R.Control of cellulose synthase complex localization in developing xylem[J].The Plant Cell,2003,15(8):1740-1748.
8. 关录凡.白桦纤维素合成酶基因 BpCesA4 的遗传转化[D].哈尔滨:东北林业大学,2009. Guan L F.Genetic transformation of birch cellulose synthase gene BpCesA4[D].Harbin:Northeast Forestry University,2009.
9. Taylor F W.Fiber length measurements-an accurate inexpensive-technique[J].Tapp,1975,12(58):126-127.
10. 李斌,顾万春,夏良放,等.鹅掌楸种源材性遗传变异与选择[J].林业科学,2001,37(2):42-50. Li B,Gu W C,Xia L F,et al.Study on genetic variation and selection of main wood characteristics among provenances of liriodendron[J].Forestry Science,2001,37(2):42-50.
11. Strimmer K,von Haeseler A.Quartet puzzling:a quartet maximum-likelihood method for reconstructing tree topologies[J].Molecular Biology and Evolution,1996,13(7):964-969.
12. Tamura K,Dudley J,Nei M,et al.MEGA4:molecular evolutionary genetics analysis(MEGA) software version 4.0[J].Molecular Biology and Evolution,2007,24(8):1596-1599.
13. Richmond T A,Somerville C R.The cellulose synthase superfamily[J].Plant Physiology,2000,124(2):495-498.
14. Yang X,Sun C,Hu Y,et al.Molecular cloning and characterization of a gene encoding RING zinc finger ankyrin protein from drought-tolerant Artemisia desertorum[J].Journal of Biosciences,2008,33(1):103-112.
15. Ranik M,Myburg A A.Six new cellulose synthase genes from Eucalyptus are associated with primary and secondary cell wall biosynthesis[J].Tree Physiology,2006,26(5):545-556.
16. Appenzeller L,Doblin M,Barreiro R,et al.Cellulose synthesis in maize:isolation and expression analysis of the cellulose synthase(CesA) gene family[J].Cellulose,2004,11(3-4):287-299.
17. Wang L,Guo K,Li Y,et al.Expression profiling and integrative analysis of the CesA/CSL superfamily in rice[J].BMC Plant Biology,2010,10(1):282-297.
18. Yin Y,Huang J,Xu Y.The cellulose synthase superfamily in fully sequenced plants and algae[J].BMC Plant Biology,2009,9:99.
19. Richmond T.Higher plant cellulose synthases[J].Genome Biology,2000,1(4):reviews3001.1.
20. Saxena I M,Brown RM JR,Fevre M,et al.Multidomain architecture of beta-glycosyl transferases:implications for mechanism of action[J].Journal of Bacteriology,1995,177(6):1419-1424.
21. Festucci-Buselli R A,Otoni W C,Joshi C O.Structure,organization,and functions of cellulose synthase complexes in higher plants[J].Brazilian Journal of Plant Physiology,2007,19(1):1-13.
22. Roberts A W,Bushoven J T.The cellulose synthase(CESA) gene superfamily of the moss Physcomitrella patens[J].Plant Molecular Biology,2007,63(2):207-219.
23. Hamann T,Osborne E,Youngs H L,et al.Global expression analysis of CESA and CSL genes in Arabidopsis[J].Cellulose,2004,11(3-4):279-286.
24. Roberts A W,Roberts E.Cellulose synthase(CesA) genes in algae and seedless plants[J].Cellulose,2004,11(3-4):419-435.
25. Mackinnon I M,Šturcová A,Sugimoto-Shirasu S K,et al.Cell-wall structure and anisotropy in procuste,a cellulose synthase mutant of Arabidopsis thaliana[J].Planta,2006,224(2):438-448.
26. Haigler C H,Ivanova-Datcheva M,Hogan P S,et al.Carbon partitioning to cellulose synthesis[J].Plant Molecular Biology,2001,47(1-2):29-51.
27. Burn J E,Hocart C H,Birch R J,et al.Functional analysis of the cellulose synthase genes CesA1,CesA2 and CesA3 in Arabidopsis[J].Plant Physiology,2002,129(2):797-807.
28. Wang L,Guo K,Li Y,et al.Expression profiling and integrative analysis of the CESA/CSL superfamily in rice[J].BMC Plant Biology,2010,10:282.
29. Krauskopf E,Harris P J,Putterill J.The cellulose synthase gene PrCESA10 is involved in cellulose biosynthesis in developing tracheids of the gymnosperm Pinus radiata[J].Gene,2005,350(2):107-116.
30. Samuga A,Joshi C P.A new cellulose synthase gene(PtrCesA2) from aspen xylem is orthologous to Arabidopsis AtCesA7(irx3) gene associated with secondary cell wall synthesis[J].Gene,2002,296(1-2):37-44.

[1]	陈柄华, 张杰, 刘桂丰, 李思婷, 高元科, 李慧玉, 李天芳. 白桦半同胞家系纸浆材优良家系选择及选择方法评价[J]. 植物研究, 2023, 43(5): 690-699.
[2]	单超然, 陈晓慧, 丁云飞, 赵威, 卢晗, 高尚珠, 齐凤慧, 詹亚光, 曾凡锁. 水曲柳FmCCoAOMT基因在木质素合成及非生物胁迫中的功能分析[J]. 植物研究, 2023, 43(5): 768-778.
[3]	郑占敏, 商玉冰, 周广波, 肖迪, 刘轶, 由香玲. PsnHB13与PsnHB15在小黑杨中的遗传转化与功能分析[J]. 植物研究, 2023, 43(3): 340-350.
[4]	王景哲, 牛朝奎, 梁馨元, 申晨静, 尹静. 水杨酸在白桦苗期抵御盐碱胁迫中的调控作用[J]. 植物研究, 2023, 43(3): 379-387.
[5]	廖诗贤, 王宇婷, 董立本, 顾咏梅, 贾丰璘, 姜廷波, 周博如. 小黑杨转录因子PsnbZIP1应答盐胁迫功能分析[J]. 植物研究, 2023, 43(2): 288-299.
[6]	刘森尧, 贾丰璘, 国庆, 樊高锋, 周博如, 姜廷波. 小黑杨转录因子PsnbHLH162基因在盐和低温胁迫下应答分析[J]. 植物研究, 2023, 43(2): 300-310.
[7]	杜金霞, 申婷婷, 王浩然, 林一萍, 李慧玉, 张连飞. 白桦BpSPL9基因抑制表达载体的构建及遗传转化研究[J]. 植物研究, 2023, 43(1): 30-35.
[8]	黄安瀛, 夏德安, 张洋, 那冬晨, 燕青, 魏志刚. PtrWRKY51基因的克隆及抗旱表达特性分析[J]. 植物研究, 2022, 42(6): 1005-1013.
[9]	陈华峰, 代龙军, 刘明洋, 郭冰冰, 杨洪, 王立丰. 橡胶树胶乳高表达热激蛋白HbHSP90.4基因抗逆功能分析[J]. 植物研究, 2022, 42(6): 1023-1032.
[10]	李登高, 林睿, 穆青慧, 周娜, 张焱如, 白薇. 马铃薯StCRKs基因家族的鉴定分析及响应逆境信号的表达[J]. 植物研究, 2022, 42(6): 1033-1043.
[11]	刘明洋, 肖化兴, 王立丰, 梁晓宇, 张宇, 王萌. 橡胶树热激蛋白HbHSP90.8-1基因的克隆与功能分析[J]. 植物研究, 2022, 42(5): 811-820.
[12]	王宏鹏, 李一丹, 汪耀, 谭晓宇, 陈成彬, 张力鹏. 菊叶薯蓣DcPMK基因克隆及互作蛋白筛选[J]. 植物研究, 2022, 42(5): 855-865.
[13]	陈坤, 方功桂, 穆怀志, 姜静. 白桦BpPIN3基因启动子序列及应答特性分析[J]. 植物研究, 2022, 42(4): 592-601.
[14]	王雪莹, 王瑞琪, 张洋, 刘聪, 夏德安, 魏志刚. 毛果杨CNGC家族全基因组鉴定及胁迫响应分析[J]. 植物研究, 2022, 42(4): 613-625.
[15]	程赫, 田双慧, 张洋, 刘聪, 夏德安, 魏志刚. 毛果杨nsLTP基因家族全基因组水平鉴定及其表达特性分析[J]. 植物研究, 2022, 42(3): 412-423.

白桦BpCesA基因的生物信息学及表达分析

Bioinformatics and Expression Analysis of BpCesA Genes of Betula platyphylla

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价