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植物研究 ›› 2005, Vol. 25 ›› Issue (4): 427-431.doi: 10.7525/j.issn.1673-5102.2005.04.011

• 论文 • 上一篇    下一篇

黑麦中与耐盐性相关的cDNA片段的克隆与序列分析

王振英1, 彭永康1, 郑坚瑜2   

  1. 1. 天津师范大学生物系, 天津 300074;
    2. 南开大学分子生物学研究所, 天津 300071
  • 收稿日期:2004-12-20 出版日期:2005-12-15 发布日期:2016-06-14
  • 通讯作者: 彭永康 E-mail:pykcell@eyou.com
  • 作者简介:王振英(1966-),女,遗传学博士,天津师范大学生物系教授,从事分子遗传学研究及教学工作。
  • 基金资助:
    天津市重点项目(023804111)和天津市教委项目资助

Cloning and sequencing analysis on the cDNA fragment SI800 separated from rye which related with salt tolerance

WANG Zhen-Ying1, PENG Yong-Kang1, ZHENG Jian-Yu2   

  1. 1. Department of Biology, Tianjin Normal University, Tianjin 300074;
    2. Department of Life Science, Nan Kai University, Tianjin 300071
  • Received:2004-12-20 Online:2005-12-15 Published:2016-06-14

摘要: SI800(Salt Induced 800 bp, SI800)为我们用mRNA差别显示技术在盐胁迫的黑麦幼苗叶片中分离到的一个盐胁迫应答cDNA片段。利用低熔点琼脂糖凝胶电泳方法纯化了该片段,并将其连接到pGEM-Teasy vector上转化大肠杆菌JM 109,获得30个阳性克隆子,经过酶切和PCR双重鉴定后,选取2个阳性克隆子进行了测序,结果表明, SI800长735 bp, GenB ank Blast结果显示,该片段与细菌中的阿卓糖酸氧化还原酶基因的部分区段有较高的同源性。

关键词: 黑麦, 耐盐性, cDNA片段的克隆, 测序

Abstract: SI800 (Salt Induced(800 bp), SI800) was a cDNA fragment, which separated from rye leaves by DDRT-PCR.In this study, we purified SI800 by low temperature agarose electrophoresis and linked to pGEM-T vector then transformed to Escherichia coli(JM 109).30 positive clones were obtained.2 positive clones were selected randomly after test by digestion, PCR and sequenced.The result indicated that SI800 was composed of(735 bp).DNA sequence analysis showed(68%) homologue to uxaB gene for altronate oxidoreductase in GenBank.

Key words: rye, salt tolerance, cloning of cDNA fragment, sequencing