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植物研究 ›› 2012, Vol. 32 ›› Issue (4): 452-457.doi: 10.7525/j.issn.1673-5102.2012.04.013

• 论文 • 上一篇    下一篇

山麦冬及其近缘种DNA条形码研究

许金榜;张君毅*   

  1. 华侨大学生物工程与技术系,厦门 361021
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-07-20 发布日期:2012-07-20
  • 通讯作者: 张君毅
  • 基金资助:
     

Analysis of Liriope spicata and Its Related Species Based on DNA Barcoding Technique

XU Jin-Bang;ZHANG Jun-Yi*   

  1. Department of Biological Engineering &Technology,Huaqiao University,Xiamen 361021
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-07-20 Published:2012-07-20
  • Contact: ZHANG Jun-Yi
  • Supported by:
     

摘要: 通过分析山麦冬及其近缘种cpDNA trnL-F、psbA-trnH间隔区序列特点,探讨trnL-F、psbA-trnH序列作为山麦冬及其近缘种DNA条形码的潜力。分别对两者进行PCR扩增后,并进行纯化测序。其中山麦冬及其近缘种9个物种18个样品的trnL-F序列长度为355~356 bp,在山麦冬属内序列完全一致,但在沿阶草属中存在特异变异位点,可对麦冬与沿阶草两物种进行鉴别。而psbA-trnH序列长度为543~544 bp,仅在麦冬中存在特异鉴别位点。结果表明,trnL-F、psbA-trnH序列由于进化速率低、保守性强,仅适合麦冬类植物属间鉴别的DNA条形码,而在属下水平的应用有一定的局限。

关键词: 山麦冬属, 沿阶草属, trnL-F序列, psbA-trnH序列

Abstract: To discuss the potential of cpDNA trnL-F and psbA-trnH as DNA barcode of Liriope spicata(Thunberg) Loureiro and its related species, cpDNA trnL-F and psbA-trnH sequences were analysed. These two regions were respectively amplified by PCR and then purified and sequenced successfully. The results showed that the lengths of trnL-F sequences in 18 plant samples belonging to 9 species of liriopogons varied from 355 to 356 bp. There was no difference in the trnL-F of various species of liriope, but some specifically variable sites were found in this fragment, which led to the identification between Ophiopogon japonicus(Thunb.) Ker-Gawl and Ophiopogon bodinieri Levl. The length of psbA-trnH region was 543-544 bp, there were only some identification sites in O.japonicus(Thunb.) Ker-Gawl. Because of the low rate of evolution and the high conservation, these two cpDNA spacer regions could be only appropriate as a DNA barcode to identify liriopogons at generic level, but there is a degree of limit at lower taxonomic level.

Key words: Liriope, Ophiopogon, trnL-F, psbA-trnH

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