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植物研究 ›› 2011, Vol. 31 ›› Issue (1): 34-39.doi: 10.7525/j.issn.1673-5102.2011.01.007

• 论文 • 上一篇    下一篇

外源一氧化氮对铅胁迫下小麦种子萌发及幼苗生理特性的影响

魏学玲;史如霞;贾凌云;刘艳;张辉;姜羽;杨颖丽*   

  1. 西北师范大学生命科学学院,兰州 730070
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-01-20 发布日期:2011-01-20
  • 通讯作者: 杨颖丽

Effects of Exogenous Nitric Oxide on Seed Germination and Physiological Characteristics of Wheat Seedlings under Lead Stress

WEI Xue-Ling;SHI Ru-Xia;JIA Ling-Yun;LIU Yan;ZHANG Hui;JIANG Yu;YANG Ying-Li*   

  1. College of Life Science,Northwest Normal University,Lanzhou 730070
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-01-20 Published:2011-01-20
  • Contact: YANG Ying-Li

摘要:

利用室内水培实验,研究了外源一氧化氮(NO)供体硝普钠(SNP)对Pb2+处理下小麦(Triticum aestivum L.)种子萌发、幼苗生长及相关生理指标变化的影响。结果表明,Pb2+处理使小麦种子发芽势、发芽率、幼苗根长和茎长均显著降低,诱导叶绿素a、叶绿素b含量减少及叶绿素荧光参数Fv/FmFv/Fo的比值减小,25 μmol·L-1 SNP明显缓解Pb2+胁迫对种子萌发及幼苗生长的抑制作用,提高Pb2+胁迫下叶绿素a、叶绿素b含量及Fv/FmFv/Fo的比值,而100 μmol·L-1SNP无明显缓解作用。此外,25和100 μmol·L-1SNP诱导Pb2+胁迫下小麦幼苗叶片过氧化氢酶(CAT)活性增强和可溶性蛋白含量增多,但100 μmol·L-1SNP处理降低了过氧化物酶(POD)活性。结果说明,外源NO促进Pb2+胁迫下小麦种子萌发及幼苗生长,提高叶绿素和可溶性蛋白含量,诱导CAT活性升高,从而增强小麦对Pb2+胁迫的适应性。

关键词: Pb2+胁迫, 种子萌发, 叶绿素, 抗氧化酶, 小麦

Abstract: By the method of solution culture, wheat was used to investigate the effects of exogenous NO donor SNP on seed germination and seedlings physiological characteristics under Pb2+ stress. The results showed that Pb2+ treatment resulted in significant decreases in seed germination and seedling growth of wheat,and notable reduction in the levels of chlorophyll a, chlorophyll b and chlorophyll fluorescence, 25 μmol·L-1 SNP could alleviate the inhibition of Pb2+ on these indexes. No significant changes in the activities of CAT and POD were observed in comparison with the control under Pb2+ stress. However, the application of SNP induced increases of CAT activity and soluble protein content in wheat seedlings under Pb2+ treatment. Therefore, exogenous NO could alleviate the poison of Pb2+ and enhance the adaptation of wheat seedlings to Pb2+ stress.

Key words: Pb2+ stress, seed germination, chlorophyll, soluble protein, wheat

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