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植物研究 ›› 2016, Vol. 36 ›› Issue (2): 310-315.doi: 10.7525/j.issn.1673-5102.2016.02.022

• 论文 • 上一篇    下一篇

陕西9种大戟属植物红外光谱分析及其分类学意义

田兰婷;赵雪艳;王宁果;李伟;彭勇;蔡霞*   

  1. 西北大学生命科学学院,西安 710069
  • 出版日期:2016-03-15 发布日期:2016-05-20

FTIR Spectra and Their Taxonomic Significance of Nine Euphorbia Species in Shaanxi

TIAN Lan-Ting;ZHAO Xue-Yan;WANG Ning-Guo;LI Wei;PENG Yong;CAI Xia*   

  1. College of Life Sciences,Northwest University,Xi’an 710069
  • Online:2016-03-15 Published:2016-05-20

摘要: 利用傅里叶红外光谱仪测定了主产于陕西省的9种大戟属植物叶片的红外光谱,并对其指纹特征区域1 800~400 cm-1进行了二阶求导。二阶导数光谱主成分分析表明:前两个主成分的累积贡献率达77.04%,能够充分反映原始光谱的大部分信息,可作为鉴定大戟属植物的依据;同时,前两个主成分分析得到的散点分布图与聚类分析结果,均将9种大戟属植物划分为3大类,其中甘遂和续随子各自单独为一类,而地锦草、大戟、华北大戟、湖北大戟、南大戟、乳浆大戟和泽漆则归为另一类,与依据形态解剖学性状对这9种植物所作的分类结果基本一致,说明傅里叶变换红外光谱法结合主成分分析与聚类分析可以为大戟属植物的分类提供依据。

关键词: 大戟属, 红外光谱, 二阶导数光谱, 分类学意义

Abstract: We measured infrared spectra of nine raw herbs of Euphorbia L. distributed in Shaanxi Province by Fourier Transform Infrared(FTIR) spectrometer, and analyzed the secondary derivative infrared spectra in 1 800-400  cm-1. By principal component analysis of the second derivative spectra, the first two principal components accounted for 77.04%, which reflected the most information of the original spectrum and could be used for identifying nine Euphorbia species. The scattered score distributive diagram and cluster analysis based on the first two principal components showed that nine raw herbs of Euphorbia L. can be divided into three classes, E.kansui was one class, E.lathylris was the other class, and E.humifusa, E.lunulata, E.pekinensis, E.hylonama, E.jolkini, E.esula and E.helioscopia fell into another class. Our result is similar to that by morphological classification, and FTIR spectroscopy combined with principal component analysis and cluster analysis could provide evidence for the taxonomic study of Euphorbia.

Key words: Euphorbia L., FTIR spectrum, secondary derivative infrared spectrum, taxonomic significance