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植物研究 ›› 2016, Vol. 36 ›› Issue (1): 105-115.doi: 10.7525/j.issn.1673-5102.2016.01.015

• 论文 • 上一篇    下一篇

拟南芥AtJ3与PKS5相互作用参与植物ABA响应

赵菲佚;焦成瑾;陈荃;贾贞;王太术;周辉   

  1. 天水师范学院生物工程与技术学院,天水 741000
  • 出版日期:2016-01-15 发布日期:2016-05-18

AtJ3 is Involved in ABA Response through Interaction with the PKS5 Kinase in Arabidopsis

ZHAO Fei-Yi;JIAO Cheng-Jin;CHEN Quan;JIA Zhen;WANG Tai-Shu;ZHOU Hui   

  1. School of Bioengineering & Biotechnology,Tianshui Normal University,Tianshui 741000
  • Online:2016-01-15 Published:2016-05-18

摘要: 脱落酸(abscisic acid,ABA)在植物生长、发育及环境胁迫响应中有着广泛的作用。前期研究已鉴定了诸多参与植物ABA信号转导的元件。本研究以拟南芥(Arabidopsis thaliana)蛋白激酶PKS5(SOS2-like protein kinase 5)为诱饵蛋白,使用酵母双杂交筛选到与PKS5相互作用的蛋白分子伴侣AtJ3(Arabidopsis thaliana DnaJ homolog 3)。AtJ3 T-DNA突变体atj3-1与atj3-2表现出萌发期ABA表型。在外源ABA处理下,atj3-1与atj3-2种子发芽率降彽、幼苗黄化、生长矮小。atj3-1与PKS5双突变体atj3-1pks5-1、atj3-1pks5-3和atj3-1pks5-4表现出与AtJ3或PKS5突变体相同的ABA表型。亚细胞定位与转基因研究显示AtJ3与PKS5有相同的表达模式。免疫共沉淀与体外磷酸化测试确认AtJ3与PKS5存在相互作用并抑制PKS5激酶活性。研究结果表明:AtJ3与PKS5相互作用,通过抑制PKS5激酶活性共同参与植物ABA信号响应过程。

关键词: ABA响应, AtJ3, 相互作用, 磷酸化活性抑制, PKS5

Abstract: The phytohormone abscisic acid(ABA) has a wide range of important roles in plant growth and development as well as abiotic stress response. Previous studies identified the enormous components involved in ABA responses in plants. We identified a chaperon AtJ3(Arabidopsis thaliana DnaJ homolog 3; heat shock protein 40-like) using yeast two-hybrid assays in which PKS5 was used as bait. The AtJ3 T-DNA mutants atj3-1 and atj3-2 displayed ABA phenotypes. Seed germinations of atj3-1 and atj3-2 decreased, and the seedlings of them showed stunted growth and leaf chlorotic symptoms under ABA. Double mutants of atj3-1pks5-1, atj3-1pks5-3 and atj3-1pks5-4 had similar ABA phenotypes as mutants of AtJ3 or PKS5. Moreover, the assays of subcellular location and transgenic plants showed that AtJ3 has overlapping expression pattern with PKS5. By co-immunoprecipitation and in vitro phosphorylation, AtJ3 physically interacts with PKS5 and represses the PKS5 kinase activity. Therefore, AtJ3 interacts with PKS5 in response to ABA by repressing the PKS5 kinase activity.

Key words: ABA response, AtJ3, interaction, phosphorylation repress, PKS5