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植物研究 ›› 2016, Vol. 36 ›› Issue (1): 58-61.doi: 10.7525/j.issn.1673-5102.2016.01.008

• 论文 • 上一篇    下一篇

合欢硬枝扦插生根解剖及相关酶活性变化研究

周祥明;刘玉堂;赵宪争;宋兆伟;王姝   

  1. 天津市农业生物技术研究中心,天津 300384
  • 出版日期:2016-01-15 发布日期:2016-05-18

Rooting Anatomy of Hardwood Cutting for Albizia julibrissin Duraxx. and Activity Change of Related Enzymes During Rooting Process

ZHOU Xiang-Ming;LIU Yu-Tang;ZHAO Xian-Zheng;SONG Zhao-Wei;WANG Shu   

  1. Tianjin Research Center of Agricultural Biotechnology,Tianjin 300384
  • Online:2016-01-15 Published:2016-05-18

摘要: 以合欢1年生硬枝为试材,通过石蜡切片法对合欢插穗不定根发生进行解剖学研究,同时,利用比色法对扦插后不同时间的材料进行了过氧化物酶(POD)、多酚氧化酶(PPO)和吲哚乙酸氧化酶(IAAO)测定。结果表明:合欢属于诱导型生根,观察到其不定根原基起源于形成层;在整个生根过程中,处理组和对照组的POD、PPO和IAAO酶活变化趋势基本一致,但3种酶活均高于对照。

关键词: 合欢, 扦插, 不定根, 解剖, 酶活性

Abstract: With one-year hardwood cutting from Albizia julibrissin Duraxx. trees, we studied the anatomical structure during the formation of adventitious roots by paraffin section, and detected the activities of peroxidase(POD), polyphenol oxidase(PPO) and indoleaceticacid oxidase by colorimetry. The adventitious root of A.julibrissin cuttings was derived from a type of induced root primordium, and the primordium of adventitious root originated from the cambium. The enzyme activities of POD, PPO and IAAO showed the same trends in treatment and control during rooting process, but the activities of three enzymes in treatment were higher than those in control.

Key words: Albizia julibrissin, Cutting, adventitious root, anatomy, enzymatic activity