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植物研究 ›› 2013, Vol. 33 ›› Issue (6): 690-696.doi: 10.7525/j.issn.1673-5102.2013.06.009

• 论文 • 上一篇    下一篇

干旱胁迫下杨树嫩茎质外体内源激素的响应

邓绍立1;姜国斌2*;任贤1;金华2;马金龙2;邹吉祥2   

  1. 1.北方民族大学生物科学与工程学院,银川 750021;2.大连民族学院生物技术与资源利用国家民委教育部重点实验室,大连 116600
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2013-11-20 发布日期:2013-11-20
  • 通讯作者: 姜国斌
  • 基金资助:
     

Responses of Endogenous Hormones to Drought Stress in the Apoplast of Populus

DENG Shao-Li;JIANG Guo-Bin*;REN Xian;JIN Hua;MA Jin-Long;ZOU Ji-Xiang   

  1. 1.College of Biological Science and Engineering,Beifang University of Nationalities,Yinchuan 750021;2.Key Laboratory of Biotechnology & Bio-Resources Utilization,State Ethnic Affairs-Commission and Ministry of Education,Dalian Nationalities University,Dalian 116600
  • Received:1900-01-01 Revised:1900-01-01 Online:2013-11-20 Published:2013-11-20
  • Contact: JIANG Guo-Bin
  • Supported by:
     

摘要: 质外体与植物细胞有着不可分割的联系,其内发生的干旱胁迫响应鲜见报道,因此本文采用MDHPLC联用技术对3种杨树嫩茎质外体内源激素在干旱胁迫胁迫下的变化进行研究。结果表明: 随着干旱胁迫程度的加剧和时间的延长,3种杨树质外体GA3、6-BA和3-IAA含量明显减少,而ABA含量极显著增加且GA3、6-BA、3-IAA和ABA含量的变化品种间差异显著。该研究为植物干旱胁迫生理响应机制研究提供新依据,为活体、动态地定量分析质外体内源激素提供了新方法。

关键词: MD-HPLC, 质外体, 内源激素, 干旱胁迫

Abstract: The apoplast and plant cells are inextricably linked, yet the response to drought stress within apoplast has rarely been reported. In this paper, the drought stress responses of apoplastic endogenous hormones in tender stems of three poplar species were studied by using MD-HPLC coupled technique. Four kinds of endogenous hormones in the apoplast of tender stems of living poplar which were dialyzed by microdialysis probe, i.e. benzylaminopurine (6-BA), gibberellic acid (GA3), indole aceticacid (3-IAA), and abscisic acid (ABA), were then simultaneously determined by HPLC. The results show that drought stress has a significant effect on the content of endogenous hormones within the apoplast (P<0.05). As the drought stress was intensified and lasting, the contents of GA-3, 6-BA and 3-IAA obviously decreased while the content of ABA increased significantly (P<0.01), meanwhile the contents of GA3, 6-BA, 3-IAA and ABA has changed differently among poplar species. This paper has provided a new basis for study on physiological response mechanism to drought stress and it also provides a new method for the dynamically quantitative analysis of the apoplastic endogenous hormone in vivo.

Key words: MD-HPLC, apoplast, endogenous hormones, drought stress

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