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植物研究 ›› 2009, Vol. 29 ›› Issue (6): 714-720.doi: 10.7525/j.issn.1673-5102.2009.06.013

• 论文 • 上一篇    下一篇

Pb2+胁迫对两种小麦幼苗生理特性影响的研究

魏学玲;史如霞;杨颖丽*;王效维;张园园;刘艳;张辉   

  1. (西北师范大学生命科学学院,兰州 730070)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-11-20 发布日期:2009-11-20
  • 通讯作者: 杨颖丽
  • 基金资助:
     

Effects of Pb2+ Stress on Physiological Characteristics of Two Wheat Seedlings

WEI Xue-Ling;SHI Ru-Xia;YANG Ying-Li*;WANG Xiao-Wei;ZHANG Yuan-Yuan;LIU Yan;ZHANG Hui   

  1. (College of life science,Northwest Normal University,Lanzhou 730070)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-11-20 Published:2009-11-20
  • Contact: YANG Ying-Li
  • Supported by:
     

摘要: 以两种小麦西旱2号和宁春4号幼苗为供试材料,研究了不同浓度Pb(NO3)2处理对其叶绿素含量、抗氧化酶活性及渗透性调节物等生理特性的影响。结果发现,两种小麦在低浓度铅处理下幼苗叶片叶绿素含量及超氧化物歧化酶(SOD)活性均无显著变化,而高浓度铅胁迫使其叶绿素a(Chla)、叶绿素b(Chlb)及叶绿素总量明显减少,但SOD活性显著升高,且相同浓度铅胁迫对小麦宁春4号幼苗叶片叶绿素的破坏作用明显强于对西旱2号的作用;不同浓度硝酸铅处理诱导两种小麦幼苗过氧化氢酶(CAT)、过氧化物酶(POD)和抗坏血酸过氧化物酶(APX)活性升高,但不影响丙二醛(MDA)含量;此外,Pb2+处理使小麦幼苗叶片脯氨酸含量升高,此效应具有浓度依赖性,但铅处理不影响可溶性糖相对含量。结果表明,铅胁迫对两种小麦幼苗叶片叶绿素造成了破坏,却不同程度诱导抗氧化酶活性及脯氨酸含量升高,即表现出较强的抗氧化能力和渗透调节能力,增强了小麦对铅的耐受性,因而胁迫诱导两种小麦叶片MDA含量变化与对照比无显著性差异。

关键词: Pb2+胁迫, 小麦, 叶绿素, 抗氧化酶, 脯氨酸

Abstract: The changes of chlorophyll content, antioxidant enzymes and osmotic regulations were investigated in two wheat seedlings, Xi Han and Ning Chun, treated with different concentrations of Pb(NO3)2. The results showed that there was no significant change of chlorophyll content and superoxide dismutase(SOD) activity in two wheat seedlings exposed to lower Pb(NO3)2 concentration, while the amount of chlorophyll a, chlorophyll b and total chlorophyll decreased and SOD activity significantly increased when wheat seedlings were subjected to higher Pb(NO3)2 concentration. The destroy to chlorophyll of Ning Chun was more serious than that of Xi Han in responses to the same Pb(NO3)2 stress. Different concentration of Pb(NO3)2 induced enhanced activities of catalase, peroxidase and ascorbate peroxidase, but no significant changes in malondialdehyde(MDA) content. In addition, the amount of proline increased by Pb(NO3)2 in two wheat seedlings in a concentrationdependent manner, but no change in soluble sugar content. The results suggested that chlorophyll content was destroyed and the activities of antioxidases and the amount of proline were enhanced in wheat seedlings under the stress of Pb(NO3)2, thus the stronger effects of antioxidanted role and osmotic regulations existed and enhanced wheat seedlings tolerance to Pb2+ stress. Therefore, there was no significant difference in MDA content between the control and Pb(NO3)2-stressed wheat seedlings.

Key words: Pb2+ stress, wheat, chlorophyll, antioxidant enzyme, proline

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