湿加松亲本间SNP遗传距离与生长性状杂种优势关系研究

doi:10.7525/j.issn.1673-5102.2021.05.012

摘要/Abstract

摘要：

通过估算湿加松亲本间SNP位点遗传距离，以有效预测杂交后代的树高、胸径、材积杂种优势，为分子辅助交配设计育种提供参考。利用SLAF-seq技术对131个湿加松亲本种质资源进行测序，获得有效的SNP标记；基于SNP位点信息，利用MEGA5.0软件估算13个湿加松亲本间遗传距离，并进行聚类分析，同时利用SPSS软件分析13个杂交组合生长性状杂种优势与SNP遗传距离的相关性。SLAF-seq共获得53 952个多态性SLAF标签，96 736个有效SNP标记；湿加松亲本间遗传距离介于0.425 1～0.490 6，亲本间SNP遗传距离与树高、胸径、材积生长性状杂种优势相关系数分别为0.680、0.648、0.624，均达到显著正相关水平。SLAF-seq技术可提供海量SNP位点，根据海量SNP位点信息可估算湿加松亲本间遗传距离，以有效预测树高、胸径、材积杂种优势。

关键词: 湿加松, SNP标记, 遗传距离, 杂种优势

Abstract:

Using SNP loci to estimate the genetic distance between the parents of Pinus elliottii × P. caribaea， and effectively predicting the heterosis of tree height， diameter at breast height（DBH） and timber volume in their hybrids， which provides a reference for molecular marker-assisted mating design and breeding. Using SLAF-seq technology to sequence and analyze the DNAs of 131 germplasm resources from the parents of P. elliottii × P. caribaea， and valid SNP markers were obtained. Based on these SNP loci， the genetic distances between 13 parents of P. elliottii × P. caribaea were estimated by using MEGA 5.0 software， and cluster analysis were performed. The heterosis of the growth traits and the correlation between heterosis and SNP genetic distance of the 13 cross combinations were analyzed and calculated by using SPSS software. A total of 53 952 polymorphic SLAF tags and 96 736 valid SNP markers were obtained by SLAP-seq. The genetic distance between the parents of P. elliottii × P. caribaea was arranged from 0.425 1 - 0.490 6， and the correlation coefficients between SNP genetic distance of the parents and heterosis of their hybrids in tree height， DBH and timber volume were 0.680， 0.648 and 0.624， respectively. All three correlation coefficients reached a significantly positive correlation level. Utilizing SLAF-seq technology can obtain massive SNP loci. Based on these SNPs， the genetic distance between parents of P. elliottii × P. caribaea can be estimated， and the heterosis of tree height， DBH and timber volume in the hybrids can be effectively predicted.

Key words: Pinus elliottii × P.caribaea, SNP marker, genetic distance, heterosis

中图分类号:

S791.246

李义良, 赵奋成, 刘阳, 钟岁英, 林昌明, 谭志强, 李福明, 廖仿炎, 吴惠姗, 郭文冰, 王哲. 湿加松亲本间SNP遗传距离与生长性状杂种优势关系研究[J]. 植物研究, 2021, 41(5): 738-743.

Yi-Liang LI, Fen-Cheng ZHAO, Yang LIU, Sui-Ying ZHONG, Chang-Ming LIN, Zhi-Qiang TAN, Fu-Ming LI, Fang-Yan LIAO, Hui-Shan WU, Wen-Bin GUO, Zhe WANG. Relationship Between Parental Genetic Distance and Heterosis of Growth Traitsin Pinus elliottii × P.caribaea var. Hondurensis Based on SNP marker[J]. Bulletin of Botanical Research, 2021, 41(5): 738-743.

图/表 5

表1

图1

表2

表3

表4

参考文献 23

1	赵奋成，李宪政，张应中，等.湿地松与洪都拉斯加勒比松的杂交效果分析［J］.林业科学研究，2006，19（4）：409-415.
	Zhao F C，Li X Z，Zhang Y Z，et al.Hybridizing effect analysis between Pinuselliottii var.elliottii and Pinuscaribaea var.hondurensis［J］.Forest Research，2006，19（4）：409-415.
2	李义良，赵奋成，吴惠姗，等.湿加松亲本间遗传距离与杂种优势的相关性分析［J］.林业科学研究，2012，25（2）：138-143.
	Li Y L，Zhao F C，Wu H S，et al.Relationship between growth traits heterosis and genetic distance among parents of Pinus elliottii × P.caribaea based on SSR molecular markers［J］.Forest Research，2012，25（2）：138-143.
3	张一，储德裕，金国庆，等.马尾松亲本遗传距离与子代生长性状相关性分析［J］.林业科学研究，2010，23（2）：215-220.
	Zhang Y，Chu D Y，Jin G Q，et al.Relationship between hybrid performance and genetic distances among Pinus massoniana clones based on ISSR molecular marker［J］.Forest Research，2010，23（2）：215-220.
4	王茜，张冬林，杨模华，等.马尾松优良家系优株ISSR遗传距离分析［J］.中南林业科技大学学报，2013，33（12）：72-76.
	Wang Q，Zhang D L，Yang M H，et al.ISSR analysis of genetic distance of Pinus massoniana Lamb.Elite clones［J］.Journal of Central South University of Forestry & Technology，2013，33（12）：72-76.
5	董虹妤，刘青华，周志春，等.马尾松子代生长杂种优势与亲本配合力、遗传距离的相关性［J］.林业科学，2017，53（2）：65-75.
	Dong H Y，Liu Q H，Zhou Z C，et al.Correlation between heterosis in the growth of progeny and combining ability and genetic distance of the parents for Pinus massoniana［J］.Scientia Silvae Sinicae，2017，53（2）：65-75.
6	Zhang Y X，Wang L H，Xin H G，et al.Construction of a high-density genetic map for sesame based on large scale marker development by specific length amplified fragment（SLAF） sequencing［J］.BMC Plant Biology，2013，13：141.
7	Qi Z M，Huang L，Zhu R S，et al.A high-density genetic map for soybean based on specific length amplified fragment sequencing［J］.PLoS One，2014，9（8）：e104871.
8	Huang S X，Ding J，Deng D J，et al.Draft genome of the kiwifruit Actinidia chinensis［J］.Nature Communications，2013，4：2640.
9	Sun X W，Liu D Y，Zhang X F，et al.SLAF-seq：an efficient method of large-scale de novo SNP discovery and genotyping using high-throughput sequencing［J］.PLoS One，2013，8（3）：e58700.
10	Riedelsheimer C，Czedik-Eysenberg A，Grieder C，et al.Genomic and metabolic prediction of complex heterotic traits in hybrid maize［J］.Nature Genetics，2012，44（2）：217-220.
11	桑世飞，王会，梅德圣，等.利用全基因组SNP芯片分析油菜遗传距离与杂种优势的关系［J］.中国农业科学，2015，48（12）：2469-2478.
	Sang S F，Wang H，Mei D S，et al.Correlation analysis between heterosis and genetic distance evaluated by genome-wide SNP chip in Brassica napus［J］.Scientia Agricultura Sinica，2015，48（12）：2469-2478.
12	Pavlov J，Delić N，Živanović T，et al.Relationship between genetic distance，specific combining abilities and heterosis in maize（Zea mays L.）［J］.Genetika，2016，48（1）：165-172.
13	Baril C P，Verhaegen D，Vigneron P，et al.Structure of the specific combining ability between two species of Eucalyptus.I.RAPD data［J］.Theoretical and Applied Genetics，1997，94（6）：796-803.
14	李善文，张志毅，于志水，等.杨树杂交亲本分子遗传距离与子代生长性状的相关性［J］.林业科学，2008，44（5）：150-154.
	Li S W，Zhang Z Y，Yu Z S，et al.Correlation between molecular genetic distances among parents and growth traits of progenies in Populus［J］.Scientia Silvae Sinicae，2008，44（5）：150-154.
15	Zhang T，Ni X L，Jiang K F，et al.Relationship between heterosis and parental genetic distance based on molecular markers for functional genes related to yield traits in rice［J］.Rice Science，2010，17（4）：288-295.
16	Wegary D，Vivek B，Labuschagne M.Association of parental genetic distance with heterosis and specific combining ability in quality protein maize［J］.Euphytica，2013，191（2）：205-216.
17	王晓阳.利用SSR分子标记探测鹅掌楸杂种优势［D］.南京：南京林业大学，2009.
	Wang X Y.Studies on the Heterosis of Liriodendron by SSR markers［D］.Nanjing：Nanjing Forestry University，2009.
18	Kaur K，Dhillon S K，Gill B S，et al.Association of SSR based genetic distances with heterosis in sunflower［J］.Journal of Environmental Biology，2019，40（5）：1102-1108.
19	Chowdari K V，Venkatachalam S R，Davierwala A P，et a1.Hybrid performance and genetic distance as revealed by the（GATA）₄ microsatellite and RAPD markers in pearl millet［J］.Theoretical and Applied Genetics，1998，97（1）：163-169.
20	Sarita K P，Tapash D，Abhishek R，et al.Relationship of parental genetic distance with heterosis and specific combining ability in sesame（Sesamum indicum L.） based on phenotypic and molecular marker analysis［J］.Biochemical Genetics，2018，56（3）：188-209.
21	Melchinger A E，Lee M，Lamkey K R，et al.Genetic diversity for restriction fragment length polymorphisms：relation to estimated genetic effects in maize inbreds［J］.Crop Science，1990，30（5）：1033-1040.
22	Charcosset A，Lefort-Buson M，Gallais A.Gallais A.Relationship between heterosis and heterozygosity at marker loci：a theoretical computation［J］.Theoretical and Applied Genetics，1991，81（5）：571-575.
23	Kwon S J，Ahn S N，Jeong E G，et al.Relationship between genetic divergence and hybrid performance in japonica rice grown in a cold water-irrigated field［J］.Euphytica，2002，128（3）：389-396.

亲本 Parent	A06	B20	B118	SS05-2	HL09	HL17	HQ09	ZH03
A06	—
B20	0.098 0	—
B118	0.085 9	0.084 6	—
SS05-2	0.135 9	0.133 9	0.125 2	—
HL09	0.483 1	0.479 7	0.437 8	0.461 7	—
HL17	0.490 6	0.479 3	0.449 6	0.466 2	0.242 0	—
HQ09	0.459 9	0.456 4	0.425 1	0.442 1	0.251 4	0.255 4	—
ZH03	0.479 5	0.472 9	0.433 8	0.464 9	0.237 0	0.233 6	0.244 6	—

杂交组合 Cross	树高 Tree height （m）	胸径 Diameter at breast height（cm）	材积 Volume of timbet（m³）
A06×HL17	9.91	16.73	0.1118
A06×HQ09	9.15	15.06	0.0856
A06×ZH3	9.29	15.27	0.091
B118×HL09	9.30	15.14	0.0897
B118×HL17	9.25	15.15	0.0886
B118×HQ09	8.99	15.17	0.0866
B118×ZH3	9.10	15.40	0.0928
B20×HL17	9.20	14.67	0.0809
B20×HQ09	9.48	14.60	0.0872
B20×ZH3	9.71	16.27	0.1013
SS05-2×HL17	8.96	14.79	0.0831
SS05-2×HQ09	8.88	14.36	0.0816
SS05-2×ZH3	9.24	14.91	0.0862

亲本组合 Combination	树高 Tree height （m）	胸径 Diameter at breast height（cm）	材积 Volume （m³）
A06×HL17	30.45	73.58	261.74
A06×HQ09	20.39	56.26	176.90
A06×ZH3	22.17	58.45	194.41
B118×HL09	22.33	57.08	190.30
B118×HL17	21.71	57.17	186.54
B118×HQ09	18.28	57.45	180.33
B118×ZH3	19.78	59.79	200.29
B20×HL17	21.00	52.18	161.85
B20×HQ09	24.78	51.49	182.24
B20×ZH3	27.73	68.81	227.67
SS05-2×HL17	17.88	53.44	168.84
SS05-2×HQ09	16.87	49.03	163.92
SS05-2×ZH3	21.64	54.67	178.84
均值Average value	21.92	57.65	190.30

[1]	周晨晨, 李义良, 王哲, 赵奋成, 钟岁英, 林昌明, 谭志强, 李福明, 吴惠姗, 郭文冰, 廖仿炎, 王建革. 基于RNA-Seq技术的湿加松特定时期产脂差异基因筛选及表达分析[J]. 植物研究, 2021, 41(3): 419-428.
[2]	章平生, 江锡兵, 龚榜初, 徐阳, 赖俊声, 吴聪连. 板栗与锥栗杂交F₁代叶片表型变异及杂种优势研究[J]. 植物研究, 2021, 41(2): 281-294.
[3]	郭文丽, 李义良, 赵奋成, 铁军, 廖仿炎, 钟岁英, 林昌明, 叶威方. 湿加松无性系表型遗传多样性研究[J]. 植物研究, 2019, 39(2): 259-266.
[4]	狄林楠, 李新蓉, 宋楠. 基于SCoT分子标记的裸果木遗传多样性分析[J]. 植物研究, 2018, 38(5): 725-732.
[5]	李树春1；夏辉2；严冬2；孟丙南3；姚立新3；刘婷婷4；崔永洪5；程玉飞6；赵曦阳2,5*. 不同派别杨树无性系SSR遗传距离及聚类分析[J]. 植物研究, 2015, 35(1): 68-76.
[6]	刘晓颖;段爽;王振英;高越;彭永康*. 由细胞质雄性不育系（CMS）配制的小麦F1杂种优势形成机理的比较蛋白质组分析[J]. 植物研究, 2013, 33(1): 51-57.
[7]	齐　明. 杉木远交亲本群体遗传多样性研究[J]. 植物研究, 2008, 28(3): 299-303.
[8]	石福臣, 木佐贯博光, 铃木和夫. 中国东北落叶松属植物亲缘关系的研究[J]. 植物研究, 1998, 18(1): 55-62.