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植物研究 ›› 2021, Vol. 41 ›› Issue (3): 449-457.doi: 10.7525/j.issn.1673-5102.2021.03.016

• 研究报告 • 上一篇    下一篇

甘草叶片形态结构和光合作用对干旱胁迫的响应

张东1,2, 刘艳1(), 张晗1, 张子健1, 王洋1, 刘美岑1   

  1. 1.内蒙古农业大学园艺与植物保护学院,呼和浩特 010011
    2.内蒙古农业大学内蒙古野生特有蔬菜种质资源与种质创新重点实验室,呼和浩特 010011
  • 收稿日期:2020-01-14 出版日期:2021-05-20 发布日期:2021-03-24
  • 通讯作者: 刘艳 E-mail:zgnly@163.com
  • 作者简介:张东(1993—),男,博士研究生,主要从事园艺植物种质资源保存及植物逆境生理研究。
  • 基金资助:
    国家自然科学基金项目(31560123)

Response of Photosynthesis and Leaf Morphological Characteristics to Drought Stress in Glycyrrhiza uralensis

Dong ZHANG1,2, Yan LIU1(), Han ZHANG1, Zi-Jian ZHANG1, Yang WANG1, Mei-Cen LIU1   

  1. 1.College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Hohhot 010011
    2.Inner Mongolia Key Laboratory of Germplasm and Germplasm Enhancement of Wild and Special Vegetable Inner Mongolia Agricultural University, Hohhot 010011
  • Received:2020-01-14 Online:2021-05-20 Published:2021-03-24
  • Contact: Yan LIU E-mail:zgnly@163.com
  • About author:ZHANG Dong(1993—),male,PhD candidate,mainly engaged in the conservation of horticultural plant germplasm resources and the physiological study of plant stress.
  • Supported by:
    The National Natural Science Foundation of China(31560123)

摘要:

叶片结构在植物防御生物和非生物胁迫方面起着重要的作用,可通过合成、储存和分泌次生代谢产物提高植物抗性。以甘草幼苗为试材,采用盆栽控水自然干旱法,探讨叶片光合作用、气孔微形态和腺体形态对干旱胁迫的响应。结果表明:①随着干旱胁迫程度的加剧,叶片净光合速率(Pn)、气孔导度(Gs)和蒸腾速率(Tr)均呈先升高后降低的趋势;其中胞间CO2浓度(Ci)在重度干旱胁迫(severe stress,SS)时迅速增高。②随着干旱胁迫程度的加剧,叶片总气孔密度和气孔开张比呈先增大后减小的趋势;而气孔开张宽度呈逐渐减小的趋势。③随着干旱胁迫程度的加剧,叶片上表皮和下表皮腺体密度总数整体上呈增大的趋势,腺体颜色随着干旱胁迫程度的加剧逐渐加深,形状出现不规则褶皱和内陷。总之,甘草叶片表面的腺体特征参与抗旱逆境调节,从而避免干旱胁迫对甘草植株的伤害;在SS下,胁迫程度加速了气孔细胞的程序性死亡(PCD),甘草幼苗失去抗旱能力。

关键词: 甘草, 干旱胁迫, 光合作用, 气孔, 腺体

Abstract:

The changes of photosynthesis, stomatal changes and glandular morphologic of leaves in Glycyrrhiza uralensis were measured with pot-culture drought stress in greenhouse, and the drought resistance of them were evaluated by membership function and drought resistance coefficient. The response of leaf photosynthesis, stomatal micromorphology and glandular morphology to drought stress was studied by pot controlled water natural drought method. The results showed that leaf net photosynthetic rate(Pn), stomatal conductance(Gs) and transpiration rate(Tr) have been rising since the degree of drought resistance after the trend of lower, and the intercellular CO2Ci) concentration increased rapidly in the severe drought stress. Moreover, leaf total stomata density and stomata opening than with the intensification of the degree of drought stress, the trend of first increases and then decreases, and stomatal opening width is gradually decreasing trend. Importantly, since the degree of drought stress, leaf epidermis and lower epidermis gland density overall showed a trend of increase in the total number of gland color as the degree of drought stress increased gradually deepened, shape appears irregular fold and retraction. The glandular characteristics on the leaf surface of G. uralensis are involved in the regulation of drought resistance, so as to avoid the damage of drought stress to G.uralensis. Under severe drought stress, stress accelerated the programmed death of stomatal cells(PCD), and G.uralensis seedlings lost their ability to resist drought.

Key words: Glycyrrhiza uralensis, drought stress, photosynthesis, stomatal, glands

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