白桦BpJMJ18基因启动子克隆及表达分析

doi:10.7525/j.issn.1673-5102.2020.05.014

植物研究 ›› 2020, Vol. 40 ›› Issue (5): 751-759.doi: 10.7525/j.issn.1673-5102.2020.05.014

白桦BpJMJ18基因启动子克隆及表达分析

王万奇¹, 齐婉竹², 赵秋爽³, 曾栋¹, 刘轶¹, 付鹏跃¹, 曲冠证¹, 赵曦阳¹

1. 东北林业大学林木遗传育种国家重点实验室, 哈尔滨 150040;
2. 吉林师范大学生命科学学院, 四平 136000;
3. 北华大学林学院, 吉林 132013

收稿日期:2020-01-17 出版日期:2020-09-05 发布日期:2020-07-10
通讯作者: 赵曦阳,E-mail:zhaoxyphd@163.com E-mail:zhaoxyphd@163.com
作者简介:王万奇(1993-),女,硕士研究生,主要从事林木遗传育种研究。
基金资助:
国家自然科学基金面上项目（31770712）；中央高校基本科研业务费专项资金（2572017DA02）

Cloning and Expression Analysis of BpJMJ18 Gene Promoter in Betula platyphylla

WANG Wan-Qi¹, QI Wan-Zhu², ZHAO Qiu-Shuang³, ZENG Dong¹, LIU Yi¹, FU Peng-Yue¹, QU Guan-Zheng¹, ZHAO Xi-Yang¹

1. State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040;
2. School of Life Science, Jilin Normal University, Siping 136000;
3. Forestry College of Beihua University, Jilin 132013

Received:2020-01-17 Online:2020-09-05 Published:2020-07-10
Supported by:
National Natural Science Foundation of China(31770712);Fundamental Research Funds for the Central Universities(2572017DA02)

摘要/Abstract

摘要： 为探究BpJMJ18基因在植物生长发育过程中的功能，本研究利用PCR技术克隆白桦（Betula platyphylla）BpJMJ18基因的启动子，通过生物信息学分析发现，该启动子序列中除了包含TATA-box和CAAT-box等基本顺式作用元件外，还具有光响应元件和多种激素应答相关的元件；进而构建植物表达载体pBI101-BpJMJ18pro：：GUS，并用农杆菌介导的瞬时转化法侵染白桦，对转基因株系进行GUS染色分析，结果发现BpJMJ18基因启动子能够驱动GUS基因在白桦的主根、侧根、根尖、叶片的维管束和嫩茎中均检测到表达。上述结果说明白桦BpJMJ18启动子具有启动活性，可能影响植物的生长发育。

关键词: 白桦, BpJMJ18启动子, 瞬时转化, 表达分析

Abstract: In order to investigate the function of BpJMJ18 gene in plant growth and development, the BpJMJ18 promoter gene was cloned from the genome of Betula platyphylla by PCR. By bioinformatics analysis, beside the basic elements such as TATA box and CAAT box, but also light responsive elements and many hormone responsive elements. Plant expression vector pBI101-BpJMJ18pro::GUS was constructed and transient transformed into B.platyphylla by Agrobacterium mediated method; the transgenic lines were analyzed by GUS staining, and the results showed that the promoter of BpJMJ18 gene could drive GUS gene to express in the roots, leaves and stems of B.platyphylla. Therefore, the BpJMJ18 promoter of B.platyphylla has the promoter activity, which may affect the growth and development of plants.

Key words: Betula platyphylla, BpJMJ18 promoter, transient transformation, expression analysis

中图分类号:

Q943

王万奇, 齐婉竹, 赵秋爽, 曾栋, 刘轶, 付鹏跃, 曲冠证, 赵曦阳. 白桦BpJMJ18基因启动子克隆及表达分析[J]. 植物研究, 2020, 40(5): 751-759.

WANG Wan-Qi, QI Wan-Zhu, ZHAO Qiu-Shuang, ZENG Dong, LIU Yi, FU Peng-Yue, QU Guan-Zheng, ZHAO Xi-Yang. Cloning and Expression Analysis of BpJMJ18 Gene Promoter in Betula platyphylla[J]. Bulletin of Botanical Research, 2020, 40(5): 751-759.

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白桦BpJMJ18基因启动子克隆及表达分析

Cloning and Expression Analysis of BpJMJ18 Gene Promoter in Betula platyphylla

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