湿加松无性系表型遗传多样性研究

doi:10.7525/j.issn.1673-5102.2019.02.012

植物研究 ›› 2019, Vol. 39 ›› Issue (2): 259-266.doi: 10.7525/j.issn.1673-5102.2019.02.012

湿加松无性系表型遗传多样性研究

郭文丽^1,2,3, 李义良^2,3, 赵奋成^2,3, 铁军⁴, 廖仿炎^2,3, 钟岁英⁵, 林昌明⁵, 叶威方⁵

1. 山西师范大学生命科学学院, 临汾 041004;
2. 广东省森林培育与保护利用重点实验室, 广州 510520;
3. 广东省林业科学研究院, 广州 510520;
4. 长治学院生物科学与技术系, 长治 046011;
5. 广东省台山红岭种子园, 台山 529200

收稿日期:2018-06-29 出版日期:2019-03-05 发布日期:2019-03-20
通讯作者: 李义良 E-mail:yiliangli@sinogaf.cn
作者简介:郭文丽(1994-),女,硕士研究生,主要从事植物生态学和保护生物学研究。
基金资助:
广东省林业科技创新专项"湿加松优良新种质创制研究"（2015KJCX011，2017KJCX014）；山西省自然科学基金项目（2012011034-6，2010011041-2）

Phenotypic Genetic Diversity of Pinus elliottii×P.caribaea Morelet var. hondurensis Clones

GUO Wen-Li^1,2,3, LI Yi-Liang^2,3, ZHAO Fen-Cheng^2,3, TIE Jun⁴, LIAO Fang-Yan^2,3, ZHONG Sui-Ying⁵, LIN Chang-Ming⁵, YE Wei-Fang⁵

1. College of Life Science, Shanxi normal University, Linfen 041004;
2. Guangdong Provincial Key Laboratory of Silviculture, Protection and Utilization, Guangzhou 510520;
3. Guangdong Academy of Forestry, Guangzhou 510520;
4. Department of Biological Sciences and Technology, Changzhi College, Changzhi 046011;
5. Taishan Hongling Seed Orchard, Taishan 529200

Received:2018-06-29 Online:2019-03-05 Published:2019-03-20
Contact: LI Yi-Liang E-mail:yiliangli@sinogaf.cn
Supported by:
Guangdong Forestry Science and Technology Innovation Project "Research on the creation of Fine New Germplasm of Wet Jiasong"(2015KJCX0111,2017KJCX014);The Natinal Natural Science Foundation of Shanxi Province(2011034-6,2010011041-2)

摘要/Abstract

摘要： 以35个湿加松（Pinus elliottii×P.caribaea Morelet var.hondurensis）无性系为试验材料，对其8个表型性状进行相关性分析、聚类分析及主成分分析研究，并深入探讨了遗传多样性水平，为湿加松无性系利用及良种选育提供科学依据。结果表明：（1）生长性状变异系数介于12.31~29.28%，均值为22.90%；多样性指数介于1.61~1.80，均值为1.69。形质性状变异系数介于13.34~47.25%，均值为26.86%；多样性指数介于1.50~1.94，均值为1.77。表明湿加松无性系遗传变异较为丰富，遗传多样性水平较高。（2）8对表型性状两两间有11对呈极显著正相关，5对呈显著正相关。选定材积为该材料育种选择的主要指标，其次为树高。（3）采用系统聚类法在欧式距离为12的时候将35个无性系分为6大类群，部分来源相同或父本相同的聚为一类，说明它们在表型上的变异较小。（4）针对8个表型性状做主成分分析提取了3个主成分，累积贡献率达86.00%，主要代表了6个性状，以此来表达所有供试材料的综合变异情况。

关键词: 湿加松, 表型性状, 遗传多样性, 主成分分析, 聚类分析

Abstract: Thirty-five Pinus elliottii×P.caribaea Morelet var. hondurensis clones were used as experimental materials, and correlation analysis, cluster analysis and principal component analysis were performed on eight phenotypic traits to explore genetic diversity level. The level of genetic diversity was discussed to provide scientific basis for the utilization of P.elliottii×P.caribaea Morelet var. hondurensis clones and breeding of improved varieties. The results showed that: (1)The coefficient of variation of growth traits ranged from 12.31%-29.28%, the mean value was 22.90%, and the diversity index ranged from 1.61-1.80 with an average of 1.69. The coefficient of variation of shape traits was between 13.34% and 47.25%, the mean value was 26.86, and the diversity index was between 1.50 and 1.94, with a mean value of 1.77. The P.elliottii×P.caribaea Morelet var. hondurensis clones was rich in genetic variation and high in genetic diversity. (2)Among the eight pairs of phenotypic traits, eleven pairs of trait correlations were extremely positively significant, and five pairs of them were positively significant. The selected volume was the main index for breeding selection of the material, followed by tree height. (3)When the Euclidean distance was 12, thirty-five clones were divided into six groups, and some of them come from the same source or the same male parent. (4)Three principal components were extracted from the principal component analysis of eight phenotypic traits, and the cumulative contribution rate reached 86.00%, which mainly represented six traits, so as to express the comprehensive variation of all the tested materials.

Key words: Pinus elliottii×P.caribaea Morelet var. hondurensis, phenotypic traits, genetic diversity, principal component analysis, cluster analysis

中图分类号:

郭文丽, 李义良, 赵奋成, 铁军, 廖仿炎, 钟岁英, 林昌明, 叶威方. 湿加松无性系表型遗传多样性研究[J]. 植物研究, 2019, 39(2): 259-266.

GUO Wen-Li, LI Yi-Liang, ZHAO Fen-Cheng, TIE Jun, LIAO Fang-Yan, ZHONG Sui-Ying, LIN Chang-Ming, YE Wei-Fang. Phenotypic Genetic Diversity of Pinus elliottii×P.caribaea Morelet var. hondurensis Clones[J]. Bulletin of Botanical Research, 2019, 39(2): 259-266.

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湿加松无性系表型遗传多样性研究

Phenotypic Genetic Diversity of Pinus elliottii×P.caribaea Morelet var. hondurensis Clones

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