3种速生树种落叶水浸提液对走马胎幼苗生长的化感效应

doi:10.7525/j.issn.1673-5102.2018.04.011

摘要/Abstract

摘要： 林药复合种植的关键在于科学理解造林树种与药材之间是否具有增益效应，其中避免化感抑制是重要方面之一。本研究选择以民族药用植物—走马胎（Ardisia gigantifolia Stapf）为研究对象，探讨走马胎与3种速生树种尾叶桉（Eualyptus urophylla S.T.Blakely）、湿地松（Pinus elliottii Engelm.）和杉木（Cunninghamia lanceolata（Lamb.）Hook.）复合种植中存在的化感作用。结果显示：与对照组CK相比，桉树落叶水浸提液降低了走马胎株高至少2.59%，对走马胎幼苗的株高有显著性抑制作用（P<0.05），对走马胎幼苗的根长、地下部分干重以及生物量有极显著性抑制作用（P<0.01），且随着浓度的增高，抑制作用增强。湿地松落叶水浸提液对走马胎幼苗的株高、根长以及地上部分干重出现低浓度促进高浓度没有显著性影响的双浓度效应，而对走马胎幼苗的基径、地下部分干重以及生物量呈现低浓度促进高浓度抑制的双浓度效应；杉木落叶水浸提液对走马胎幼苗生长的影响呈现低浓度促进高浓度抑制的双浓度效应现象。综合各项指标得出：走马胎与桉树之间存在较强的负化感效应，而走马胎与湿地松或杉木之间存在“低促高抑”的化感效应；

关键词: 走马胎, 杉木, 湿地松, 桉树, 化感作用, 复合种植

Abstract: It is the key to understand scientifically the compound planting of forest medicine that whether there is gain effect between afforestation tree species and medicinal materials, among which, avoiding of allelopathic inhibition is one important aspect. Allelopathic effects of three fast-growing tree species on Ardisia gigantifolia Stapf were evaluated to discuss if A.gigantifolia was mixed with Eucalyptus urophylla S.T.Blakely, Pinus elliottii or Cunninghamia lanceolata(Lamb.) Hook. Comparing with CK, the plant height of A.giantifolia was significantly lower under the treatments of 40, 60 and 80 g·L^-1(P<0.05), and the root length, dry weight underground and biomass were also strongly inhibited under all the treatments(P<0.01); With the increase of the extract concentration, the inhibition of E.urophylla was enhanced. The aqueous extraction of P.elliottii had a positive stimulatory effect on height, root length and dry aboveground weight of A.gigantifolia under treatment of the 20 and 40 g·L^-1, while the higher concentration had weak or negative effect. Besides, P.elliottii had a stimulatory effect on basal diameter, biomass and dry underground weight of A.gigantifolia under treatment of the lowest content, while the highest content had an inhibitory effect. For C.lanceolata the lowest content had a stimulatory effect, while the highest concentration showed significant inhibition. Taking all parameters into account, Eucalyptus could not be mixed with A.giantifolia, while P.elliottii or C.lanceolata may be an alternative intercropping plant species for A.giantifolia, because the growth of A.giantifolia was inhibited significantly by the allelochemicals released from the decomposing leaf litter of Eucalyptus, and the growth of A.giantifolia was stimulated under the lowest content of aqueous extraction from P.elliottii and C.lanceolata.

Key words: Ardisia giantifolia Staf, Cunninghamia lanceolata(Lamb.), Pinus elliottii, Eucalyptus, allelopathy, intercropping

中图分类号:

S718.52

周泽建, 刘妮妮, 伍冰倩, 张红, 赵珩. 3种速生树种落叶水浸提液对走马胎幼苗生长的化感效应[J]. 植物研究, 2018, 38(4): 568-574.

ZHOU Ze-Jian, LIU Ni-Ni, WU Bing-Qian, ZHANG Hong, ZHAO Heng. Allelopathic Effects of Leaves Extracts on Growth of Three Fast-growing Tree Species on Ardisia gigantifolia Staf Seedlings[J]. Bulletin of Botanical Research, 2018, 38(4): 568-574.

参考文献

1. 周泽建. 林药间作研究现状与展望[J]. 广西农学报,2016,31(5):55-58.Zhou Z J. A review of the present situation and future prospect of forest-medicinal herb intercropping[J]. Journal of Guangxi Agriculture,2016,31(5):55-58.
2. 李茜. 渭北黄土区农林复合系统核桃化感作用研究[D]. 杨凌:西北农林科技大学,2011:1-23.Li Q. Allelopathic effects of walnut on agroforestry system in loess area of Northern Wei river in Shaanxi[D]. Yangling:Northwest A&F University,2011:1-23.
3. 贺维,陈刚,陈洪,等. 美洲黑杨凋落叶分解初期对小白菜生长的影响[J]. 生态学报,2015,35(7):2067-2075.He W,Chen G,Chen H,et al. Effects of decomposing leaf litter of Populus deltoides on the growth and physiology of Brassica chinensis[J]. Acta Ecologica Sinica,2015,35(7):2067-2075.
4. Rice E L. Allelopathy[M]. New York:Academic Press,1974:292-293.
5. Uddin M N,Robinson R W,Buultjens A,et al. Role of allelopathy of Phragmites australis in its invasion processes[J]. Journal of Experimental Marine Biology and Ecology,2017,486:237-244.
6. 朱宇林,谭萍,陆绍锋,等. 桉树化感效应下Si对植物种子萌发的影响[J]. 种子,2010,29(8):19-22.Zhu Y L,Tan P,Lu S F,et al. Effect of Silicone on plant seed germination under the allelopathic effects of Eucalyptus leaves water extract[J]. Seed,2010,29(8):19-22.
7. 李培泰. 良种桉树叶中黄酮类物质的提取和活性试验报告[J]. 广西林业,1998(3):15-16.Li P T. Extraction and activity test of flavonoids from leaves of Eucalyptus grandis[J]. Forestry of Guangxi,1998(3):15-16.
8. Chu C J,Mortimer P E,Wang H C,et al. Allelopathic effects of Eucalyptus on native and introduced tree species[J]. Forest Ecology and Management,2014,323:79-84.
9. 林捷,曾任森,石木标,等. 尾叶桉和湿地松对彩色豆马勃的化感作用研究[J]. 华南农业大学学报:自然科学版,2003,24(2):48-50.Lin J,Zeng R S,Shi M B,et al. Allelopathic effects of Eucalyptus urophylla and Pinus elliottii on Pisolithus tinctorius[J]. Journal of South China Agricultural University:Natural Science Edition,2003,24(2):48-50.
10. 王聪,张饮江,段婷,等. 3种陆生植物浸出液对水绵化感抑制作用研究[C]. //渔业科技创新与发展方式转变——2011年中国水产学会学术年会论文摘要集. 厦门:中国水产学会,2011:277.Wang C,Zhang Y J,Duan T,et al. Allelopathy and inhibitory effect of extracts from three terrestrial plants on Spirogyra growth[C]. //Academic annual meeting of China Fisheries Society. Xiamen:China Society of Fisheries,2011:277.
11. 张腾. 马尾松和杉木对植物的化感作用研究[D]. 杭州:浙江大学,2016:1-63.Zhang T. Allelopathic effect of Masson pine(Pinus massoniana Lamb. ) and Chinese fir(Cunninghamia lanceolata(Lamb. ) Hook. ) on target plants[D]. Hangzhou:Zhejiang University,2016:1-63.
12. 中国科学院中国植物志编辑委员会. 中国植物志[M]. 北京:科学出版社,1979:93.Editorial Committee of Flora of China. Flora of China[M]. Beijing:Science Press,1979:93.
13. Bruce Williamson G,Richardson D. Bioassays for allelopathy:Measuring treatment responses with independent controls[J]. Journal of Chemical Ecology,1988,14(1):181-187.
14. 陈娟,白尚斌,周国模,等. 毛竹浸提液对苦槠幼苗生长的化感效应[J]. 生态学报,2014,34(16):4499-4507.Chen J,Bai S B,Zhou G M,et al. Allelopathic effects of Phyllostachys edulis extracts on Castanopsis sclerophylla[J]. Acta Ecologica Sinica,2014,34(16):4499-4507.
15. 赵新梅,王军,莫静静,等. 3种作物茎叶枯落物水浸液对烟草幼苗生长的化感效应[J]. 草业学报,2016,25(9):37-45.Zhao X M,Wang J,Mo J J,et al. Allelopathic effects of leaf-stem litter water aqueous extracts of three plant species on tobacco seedlings[J]. Acta Prataculturae Sinica,2016,25(9):37-45.
16. Ferreira M C,De Souza J R P,De Jesus Faria T. Allelopathy of plant extracts on germination and initial growth of beggartick(Bidens pilosa L. ) and lettuce(Lactuca sativa L. )[J]. Ciência e Agrotecnologia,2007,31(4):1054-1060.
17. Wu A P,Yu H,Gao S Q,et al. Differential belowground allelopathic effects of leaf and root of Mikania micrantha[J]. Trees,2009,23(1):11-17.
18. Saxena A,Singh D V,Joshi N L. Autotoxic effects of pearl millet aqueous extracts on seed germination and seedling growth[J]. Journal of Arid Environments,1996,33(2):255-260.
19. Sun B Y,Tan J Z,Wan Z G,et al. Allelopathic effects of extracts from Solidago canadensis L. against seed germination and seedling growth of some plants[J]. Journal of Environmental Sciences,2006,18(2):304-309.
20. Arouiee H,Azizi M. Allelopathic effects of some medicinal plants extracts on seed germination of some weeds and medicinal plants[J]. International Symposium on Biocontrol and Biotechnology,2006,(8):139-147.
21. 黄洪武,李俊,董立尧,等. 加拿大一枝黄花对植物化感作用的研究[J]. 南京农业大学学报,2009,32(1):48-54.Huang H W,Li J,Dong L Y,et al. The study on allelopathy of Solidago canadensis L. to several plants[J]. Jouranal of Nanjing Agricultural University,2009,32(1):48-54.
22. 曹光球. 杉木自毒作用及其与主要混交树种化感作用的研究[D]. 福州:福建农林大学,2006:1-73.Cao G Q. Chinese fir autointoxication and interspecific allelopathy between Chinese fir and its associated tree species[D]. Fuzhou:Fujian Agriculture And Forestry University,2006:1-73.
23. 段剑,杨洁,康金林,等. 马尾松根际土壤提取物的化感作用[J]. 生态学杂志,2015,34(3):773-780.Duan J,Yang J,Kang J L,et al. Allelopathic effect of extracts from Pinus massoniana rhizosphere soil[J]. Chinese Journal of Ecology,2015,34(3):773-780.
24. 曾冀,赵志刚,郭文福,等. 马尾松化感作用的生物测定[J]. 林业实用技术,2009,(11):33-34.Zeng J,Zhao Z G,Guo W F,et al. Bioassay of allelopathy of Pinus massoniana[J]. Practical Forestry Technology,2009,(11):33-34.
25. 卢玉鹏,许纪元,张晓曦,等. 林下药材植物淋出物对太白杨枯落物分解及土壤酶活性的影响[J]. 土壤学报,2017,54(3):749-758.Lu Y P,Xu J Y,Zhang X X,et al. Effects of leachates from understory medicinal plants on decomposition of Populus purdomii litters and soil enzyme activity[J]. Acta Pedologica Sinica,2017,54(3):749-758.
26. 沈国舫,翟明普. 混交林研究:全国混交林与树种间关系学术讨论会文集[M]. 北京:中国林业出版社,1997:25-32.Shen G F,Zhai M P. Study of mixed forest[M]. Beijing:China Forestry Publishing House,1997:25-32.

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