越橘茎尖离体培养条件的研究

doi:10.7525/j.issn.1673-5102.2018.02.021

植物研究 ›› 2018, Vol. 38 ›› Issue (2): 316-320.doi: 10.7525/j.issn.1673-5102.2018.02.021

• 研究简报 • 上一篇

越橘茎尖离体培养条件的研究

李艳霞¹, 邓继峰², 李桂君¹

1. 黑龙江省林业科学研究所, 哈尔滨 150081;
2. 沈阳农业大学林学院, 沈阳 110866

收稿日期:2017-07-31 出版日期:2018-03-15 发布日期:2018-03-23
通讯作者: 李桂君 E-mail:liguijun2008123@163.com
作者简介:李艳霞(1980-),女,副研究员,主要从事林木遗传育种及改良工作。
基金资助:
黑龙江省森工总局项目（sgzjY2014008）；中国—白俄罗斯政府间科技合作项目（CB11-15）

The in vitro Culture for Vaccinium vitis-idaea Stem Apex

LI Yan-Xia¹, DENG Ji-Feng², LI Gui-Jun¹

1. Forestry Research Institute of Heilongjiang Province, Harbin 150081;
2. College of Forestry, Shenyang Agricultural University, Shenyang 110866

Received:2017-07-31 Online:2018-03-15 Published:2018-03-23
Supported by:
Heilongjiang provincial forestry bureau program(sgzjY2014008);China-Belarus-science and technology cooperation project(CB11-15)

摘要/Abstract

摘要： 为提高越橘苗木繁殖速度及苗木品质，本研究以越橘茎尖为材料，建立茎尖离体培养诱导成苗的快繁体系。结果表明：茎尖诱导最佳培养基为WPM（改良）+ZT 0.05 mg·L^-1+IBA 0.5 mg·L^-1+GA₃ 0.1 mg·L^-1，0.1~0.3 mm长的茎尖诱导成苗率仅为28%，0.3~0.5 mm长的茎尖诱导成苗率能达到68%；茎尖长度达到0.5~0.7 mm时，茎尖诱导成苗率为79%，当茎尖长度为0.7~1.0 mm时，茎尖诱导成苗率高达94%。

关键词: 越橘, 茎尖培养, 激素, 培养基

Abstract: In order to improve the breeding rate and qualities of Vaccinium vitis-idaea seedlings, in our study, V.vitis-idaea stem apexes were used as research materials, then rapid propagation systems of plants induced by stem apex in vitro culture was established. The results indicated that the optimal induced stem apex in vitro culture is WPM(improved) +ZT 0.05 mg·L^-1 +IBA 0.5 mg·L^-1 +GA₃ 0.1 mg·L^-1. The success rate of plants induced by 0.1-0.3 mm long stem apex was only 28%, while such a rate of plants induced by 0.3-0.5 mm long stem apex was up to 68%; then stem apex reached to 0.5-0.7 mm, the success rate was 79%, and the success rate of plants induced by 0.7-1.0 mm long stem apex reached up to 94%.

Key words: Vaccinium vitis-idaea L., stem apex culture, hormone, culture medium

中图分类号:

S792.132

李艳霞, 邓继峰, 李桂君. 越橘茎尖离体培养条件的研究[J]. 植物研究, 2018, 38(2): 316-320.

LI Yan-Xia, DENG Ji-Feng, LI Gui-Jun. The in vitro Culture for Vaccinium vitis-idaea Stem Apex[J]. Bulletin of Botanical Research, 2018, 38(2): 316-320.

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越橘茎尖离体培养条件的研究

The in vitro Culture for Vaccinium vitis-idaea Stem Apex

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