塔拉种子多糖脱蛋白的正交优化及其抗氧化性研究

doi:10.7525/j.issn.1673-5102.2018.01.019

植物研究 ›› 2018, Vol. 38 ›› Issue (1): 155-160.doi: 10.7525/j.issn.1673-5102.2018.01.019

• 研究报告 • 上一篇

塔拉种子多糖脱蛋白的正交优化及其抗氧化性研究

袁德成, 赵寒梅, 杨逢建

东北林业大学森林植物生态学教育部重点实验室, 哈尔滨 150040

收稿日期:2017-04-05 出版日期:2018-01-15 发布日期:2018-01-06
通讯作者: 杨逢建,E-mail:yangfj@nefu.edu.cn E-mail:yangfj@nefu.edu.cn
作者简介:袁德成(1993—),男,硕士研究生,主要从事植物资源学的研究.
基金资助:
林业公益性行业科研专项（201404616）

Study on Orthogonal Optimization and Antioxidation of Polysaccharide from Tara Seeds

YUAN De-Cheng, ZHAO Han-Mei, YANG Feng-Jian

Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040

Received:2017-04-05 Online:2018-01-15 Published:2018-01-06
Supported by:
Forestry Public Welfare Industry Research Special(201404616)

摘要/Abstract

摘要： 以塔拉（Caesalpinia spinosa）种子为原料，研究了塔拉种子多糖的脱蛋白工艺及塔拉多糖的抗氧化性质。以多糖损失率和蛋白脱除率为评价指标，比较Sevage法、三氯乙酸法和木瓜蛋白酶法对塔拉多糖的脱蛋白效果。利用正交优化组合实验设计原理，采用四因素三水平的正交分析法，对木瓜蛋白酶法脱蛋白进行正交优化。结果表明：塔拉多糖最佳脱蛋白工艺条件为酶添加量0.15mL、酶解时间90min、酶解温度60℃、酶解pH=6，蛋白脱除率95.19%，多糖保留率75.02%。通过对塔拉多糖抗氧化性的研究，发现塔拉多糖总抗氧化性较好，对DPPH自由基有较强的清除作用。

关键词: 塔拉多糖, 脱蛋白, 正交优化, 抗氧化性

Abstract: Tara seed as raw material, process and antioxidant properties of protein and polysaccharide of Tara seed polysaccharide of Tara.Using the loss rate of polysaccharide and protein removal rate as the evaluation index to compare Sevage method, three trichloroacetic acid method and papain method of Tara polysaccharide deproteinization effect. Removing protein were optimized by using orthogonal optimization experimental design principle and the method of orthogonal analysis of four factors and three levels of papain method. The results showed that: Tara polysaccharide best protein removal technology for enzyme dosage 0.15 mL, hydrolysis time 90 min, enzymolysis temperature of 60℃, enzymolysis pH=6, protein removal rate was 95.19%, rate of polysaccharide is 75.02%. Through the study of Tara polysaccharide antioxidation of polysaccharide retention at Tara, it is showed thatthe total antioxidant activity of sugar was better, which had a strong scavenging effect on DPPH free radical.

Key words: Tara polysaccharide, removing protein, orthogonal optimization, antioxidant activity

中图分类号:

Q949.751.9

袁德成, 赵寒梅, 杨逢建. 塔拉种子多糖脱蛋白的正交优化及其抗氧化性研究[J]. 植物研究, 2018, 38(1): 155-160.

YUAN De-Cheng, ZHAO Han-Mei, YANG Feng-Jian. Study on Orthogonal Optimization and Antioxidation of Polysaccharide from Tara Seeds[J]. Bulletin of Botanical Research, 2018, 38(1): 155-160.

参考文献

1. 张建云,李志国,夏定久,等. 塔拉单宁和塔拉多糖研究现状[J]. 天然产物研究与开发,2009,21(1):183-188.Zhang J Y,Li Z G,Xia D J,et al. Investigating actuality and the vista of Tara tannin and Tara polysaccharide[J]. Natural Product Research and Development,2009,21(1):183-188.
2. 蒋建新,朱莉伟,张卫明,等. 塔拉多糖胶的研究[J]. 西南林学院学报,2003,23(4):12-16.Jiang J X,Zhu L W,Zhang W M,et al. Study on properties of Caesalpinia spinosa polysaccharide gum[J]. Journal of Southwest Forestry College,2003,23(4):12-16.
3. 马文秀,罗庆云,尚征贤,等. 塔拉豆多糖研究[J]. 林产化学与工业,1999,19(4):29-34.Ma W X,Luo Q Y,Shang Z X,et al. Study on polysaccharide in Tara seed[J]. Chemistry and Industry of Forest Products,1999,19(4):29-34.
4. 张杰,孙源. 超声提取蛹虫草多糖及其抗氧化活性分析[J]. 食品科技,2013,38(5):203-207.Zhang J,Sun Y. Ultrasonic extraction and anti-oxidation of polysaccharide frommycelium of Cordyceps militaris[J]. Food Science and Technology,2013,38(5):203-207.
5. 赵鹏,安叶娟,宋逍,等. 超声提取桦黄多糖工艺及其多糖抗氧化性能研究[J]. 食品工业科技,2014,35(20):252-256.Zhao P,An Y J,Song X,et al. Study on ultrasonic wave extraction and antioxidant of polysaccharidesfrom Piptoporus betulinus Karst[J]. Science and Technology of Food Industry,2014,35(20):252-256.
6. 张媛媛,张彬. 苯酚—硫酸法与蒽酮—硫酸法测定绿茶茶多糖的比较研究[J]. 食品科学,2016,37(04):158-163.Zhang Y Y,Zhang B. Comparison of Phenol-Sulfuric Acid and Anthrone-Sulfuric Methods for Determination of Polysaccharide in Green Tea[J]. Food Science,2016,37(04):158-163.
7. 池源,王丽波. 苯酚—硫酸法测定南瓜籽多糖含量的条件优化[J]. 食品与机械,2014,30(1):89-92.Chi Y,Wang L B. Study on Phenol-Sulfuric acid method for determination of polysaccharide content in pumpkin seeds[J]. Food & Machinery,2014,30(1):89-92.
8. 武永福. 黄花菜多糖的提取工艺及含量测定[J]. 中国食物与营养,2015,21(5):54-57.Wu Y F. Study on extraction and determination of Lily polysaccharides [J]. Food and Nutrition in China,2015,21(5):54-57.
9. 路祺,高悦,项凤影,等. 牡丹种荚多糖提取工艺研究[J]. 植物研究,2015,35(1):154-157.Lu Q,Gao Y,Xiang F Y,et al. Polysaccharide Extraction from Peony Seed Pods by Water Bath[J]. Bulletin of Botanical Research,2015,35(1):154-157.
10. 刘燕,陈小银,杨丽丽,等. 响应面优化菊芋菊糖的提取工艺研究[J]. 植物研究,2016,36(4):627-633.Liu Y,Chen X Y,Yang L L,et al. Extraction Process Optimization of Inulin from Helianthus tuberosus L. by Response Surface Methodology[J]. Bulletin of Botanical Research,2016,36(4):627-633.
11. 蒋婧. 蛋白质水解度测定及其标准曲线绘制研究[J]. 广东轻工职业技术学院学报,2012,11(1):4-6.Jiang J. Study of Determination of Protein Hydrolysis Degree and Its Formation of Standard Curve[J]. Journal of Guangdong Industry Technical College,2012,11(1):4-6.
12. Omeje K O,Eze S O O,Ozougwu V E O,et al. Application of Papain from Paw Paw(Carica papaya) latex in the Hydrolysis of Tiger Nut(C. esculentus) Proteins[J]. Mitteilungen Klosterneuburg,2014:64.
13. Cao J,Covarrubias V M,Straubinger R M,et al. A rapid,reproducible,on-the-fly orthogonal array optimization method for targeted protein quantification by LC/MS and its application for accurate and sensitive quantification of carbonyl reductases in human liver[J]. Analytical Chemistry,2010,82(7):2680-2689.
14. 唐志红,王艺,马红婷,等. 浒苔多糖脱蛋白工艺的考察[J]. 食品研究与开发,2015,36(11):90-93.Tang Z H,Wang Y,Ma H T,et al,Qin Song. Investigation of Deproteinized Technology for Polysaccharide from Enteromorpha Prolifera[J]. Food Research And Development,2015,36(11):90-93.
15. 吴光杰,李玉萍,陈美琴,等. 马齿苋多糖蛋白含量测定及脱蛋白方法研究[J]. 时珍国医国药,2015,26(07):1577-1579.Wu G J,Li Y P,Chen M Q,et al. Study on Determination of Protein in Polysaccharides from Portulace oleracea L. and deproteinization methods[J]. Lishizhen Medicine and Materia MedicaResearch,2015,26(7):1577-1579.
16. 赵师师,高旭红,常君,等. 石榴皮多糖的三氯乙酸法脱蛋白工艺研究[J]. 广州化工,2015,43(17):64-66.Zhao S S,Gao X H,Chang J,et al. Liu SongLin,Zhang Ping. Study on the Deproteinization of the Crude Polysaccharide from Pomegranate Peel with Trichloroacetic Acid Method[J]. Guangzhou Chemical Industry,2015,47(17):64-66.
17. 朱思洁,吴天祥,杨祖滔,等. 灰树花胞外多糖脱蛋白工艺研究[J]. 中国酿造,2016,35(07):161-165.Zhu S J,Wu T X,Yang Z T,et al. Deproteinization technology of exopolysaccharide from Grifola frondosa[J]. China Brewing,2016,35(7):161-165.
18. 昝志惠,高艳梅,孙墨珑. 核桃楸单宁提取及其抗氧化性[J]. 植物研究,2015,35(3):431-435.Zan Z H,Gao Y M,Sun M L. Extraction and Antioxidant Activity of Tannic in Juglans mandshurica Maxim. [J]. Bulletin of Botanical Research,2015,35(3):431-435.
19. 宋晓燕,高彦祥,袁芳. 响应面法优化羊胎粉中抗氧化多肽制备工艺的研究[J]. 食品科技,2008,33(11):237-241.Song X Y,Gao Y X,Yuan F. Optimization of antioxidant peptide production from goat placenta powder using RSM[J]. Food Science and Technology,2008,33(11):237-241.

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塔拉种子多糖脱蛋白的正交优化及其抗氧化性研究

Study on Orthogonal Optimization and Antioxidation of Polysaccharide from Tara Seeds

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