3种相思16年生优树外植体芽诱导研究

doi:10.7525/j.issn.1673-5102.2016.05.014

摘要/Abstract

摘要： 以16年生马占相思、大叶相思和马大杂种相思优树为材料，研究外植体消毒、部位、收集的季节、类型、以及6-BA、蔗糖、培养基类型对芽诱导的影响，以建立3种相思成年优树高效芽诱导体系。结果表明：3种相思的出芽率受消毒剂的影响较大，马占相思对消毒剂处理最敏感（升汞9 min和酒精15 s为最佳），大叶相思和马大杂种相思较耐受消毒剂的处理（分别以升汞和酒精处理18 min，15 s和15 min，30 s为最佳）。3个树种均以第3~5个腋芽茎段为最佳外植体部位，以类型2（母株枝条先通过扦插繁殖来建立采穗圃，通过选取采穗圃中的枝条），8月采集的外植体为最佳，分别接种于最适培养基：改良MS+6-BA 0.5 mg·L^-1+蔗糖30 g·L^-1（马占相思），MS+6-BA 1.0 mg·L^-1+蔗糖40 g·L^-1（大叶相思），MS+蔗糖20 g·L^-1（马大杂种相思），进行芽诱导时，最高诱导率分别为93.33%（马占相思），92.00%（大叶相思），97.33%（马大杂种相思）。

关键词: 马占相思, 大叶相思, 马大杂种相思, 外植体, 芽诱导

Abstract: The explants from 16-year elite trees of Acacia mangium, A.auriculiformis and A.mangium×A.auriculiformis were used to establish the efficient bud inducing technique, by studying the sterilization methods, position, gathered time and type of explants, the effects of 6-BA, sucrose, and basal medium. A.mangium was disinfectant sensitive, treatment with mercuric chloride 9 min and ethanol 15 s was the optimal, but longer time were need for A.auriculiformis and A.mangium×A.auriculiformis, treatment with mercuric chloride 18 min, ethanol 15 s and mercuric chloride 15 min, and ethanol 30 s, respectively. These three Acacia species shared common optimal position, gathered season and type:3-5^th node of shoot as explants were optimal position. It was the optimal season to gather explants in August; Type 2 explant germinated from shoots of cutting orchard (established by cutting the shoots of mature elite) was the optimal type; The optimal bud inducing medium were modify MS+6-BA 0.5 mg·L^-1+sucrose 30 g·L^-1 for A.mangium, MS+6-BA 1.0 mg·L^-1+sucrose 40 g·L^-1 for A.auriculiformis, MS+ sucrose 20 g·L^-1 for A.mangium×A.auriculiformis, with the highest germination rate of 93.33%, 92.00% and 97.33%, respectively.

Key words: Acacia mangium, A.auriculiformis, A.mangium×, A.auriculiformis, explants, bud inducing

中图分类号:

Q813.1+2

王鸿, 黄烈健, 胡峰. 3种相思16年生优树外植体芽诱导研究[J]. 植物研究, 2016, 36(5): 730-738.

WANG Hong, HUANG Lie-Jian, HU Feng. Bud Inducing from 16-year Elite Trees Explants of Three Acacia Species[J]. Bulletin of Botanical Research, 2016, 36(5): 730-738.

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