干旱胁迫对红松主要次生代谢产物的含量及其DPPH清除能力的影响

doi:10.7525/j.issn.1673-5102.2016.04.009

植物研究 ›› 2016, Vol. 36 ›› Issue (4): 542-548.doi: 10.7525/j.issn.1673-5102.2016.04.009

干旱胁迫对红松主要次生代谢产物的含量及其DPPH清除能力的影响

张丹¹, 任洁¹, 刘红梅², 王慧梅¹

1. 东北林业大学森林植物生态学教育部重点实验室, 哈尔滨 150040;
2. 阜新市林业局, 阜新 123000

收稿日期:2016-03-01 出版日期:2016-07-15 发布日期:2016-06-15
通讯作者: 王慧梅,E-mail:whm0709@163.com E-mail:whm0709@163.com
作者简介:张丹(1990-),女,硕士研究生,主要从事药用植物逆境生理研究。
基金资助:
中央高校基本科研业务费专项资金(DL13EA03-03);国家自然科学基金(31100457);黑龙江省自然科学基金(C2015051)

Responses of Main Secondary Metabolites and DPPH Free Radical Scavenging Activity of the Korean Pine to Drought Stress

ZHANG Dan¹, REN Jie¹, LIU Hong-Mei², WANG Hui-Mei¹

1. Key Laboratory of Forest Plant Ecology, Northeast Forestry University, Harbin 150040;
2. Forestry Bureau of Fuxin City, Fuxin 123000

Received:2016-03-01 Online:2016-07-15 Published:2016-06-15
Supported by:
This research is supported by Special Funds for basic research of Central University(DL13EA03-03);Natural Science Foundation of China(NSFC)(31100457);Natural Science Foundation of Heilongjiang Province(C2015051)

摘要/Abstract

摘要： 以红松为材料,研究了干旱及复水对红松针叶和树皮绿色组织的黄酮、单宁和原花青素这三种次生代谢产物的含量及其提取物对DPPH清除能力的影响。结果表明:干旱胁迫下,红松树皮绿色组织黄酮、单宁和原花青素含量均显著下降,复水后黄酮和单宁含量持续降低,三种含量均未恢复到对照水平;针叶黄酮和原花青素含量明显降低,单宁含量明显升高,复水后均未恢复到对照水平。干旱胁迫使红松针叶和树皮绿色组织IC₅₀值均明显升高,相对应提取物的DPPH清除能力均明显下降,复水后针叶和树皮绿色组织提取物对DPPH清除能力恢复至对照水平。本研究结果表明干旱胁迫对红松针叶和树皮绿色组织次生代谢产物及抗氧化活性均产生一定影响,但针叶和树皮绿色组织的反应存在一定差异。

关键词: 干旱胁迫, 针叶, 树皮绿色组织, 黄酮, 单宁, 原花青素, DPPH自由基

Abstract: The Korean pine were tested to study the effects of drought stress and rehydration on flavonoids content, tannin content, procyanidin content and DPPH free radical scavenging activity of needles and bark chlorenchyma. Flavonoids, tanninand procyanidin content of bark chlorenchyma decreased significantly under drought stress, flavonoids and tannin content continue to decrease after rehydration, and three kinds of content were not returned to control levels. Flavonoids and procyanidin content of needles decreased and tannin content significantly increased, after rehydration they were not returned to control levels. IC₅₀ values of needles and bark chlorenchyma significantly increased under drought stress, and DPPH free radical scavenging activity of extract was significantly decreased, after rehydration DPPH free radical scavenging activity of needle and bark chlorenchyma were returned to control levels. Secondary metabolites and antioxidant activity of needles and bark chlorenchyma were significantly affected by drought stress, but there were some different responses to drought stress between needles and bark chlorenchyma.

Key words: drought stress, needles, bark chlorenchyma, flavonoids, tannin, procyanidin, DPPH free radical

中图分类号:

S791.247

张丹, 任洁, 刘红梅, 王慧梅. 干旱胁迫对红松主要次生代谢产物的含量及其DPPH清除能力的影响[J]. 植物研究, 2016, 36(4): 542-548.

ZHANG Dan, REN Jie, LIU Hong-Mei, WANG Hui-Mei. Responses of Main Secondary Metabolites and DPPH Free Radical Scavenging Activity of the Korean Pine to Drought Stress[J]. Bulletin of Botanical Research, 2016, 36(4): 542-548.

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干旱胁迫对红松主要次生代谢产物的含量及其DPPH清除能力的影响

Responses of Main Secondary Metabolites and DPPH Free Radical Scavenging Activity of the Korean Pine to Drought Stress

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