超声法提取山里红叶总黄酮及其抗氧化活性研究

doi:10.7525/j.issn.1673-5102.2015.04.025

植物研究 ›› 2015, Vol. 35 ›› Issue (4): 632-637.doi: 10.7525/j.issn.1673-5102.2015.04.025

超声法提取山里红叶总黄酮及其抗氧化活性研究

罗猛^1,2；宋卓悦^1,2；胡娇阳^1,2；牟璠松^1,2*；乔琪^1,2；祖元刚^1,2

1.东北林业大学森林植物生态学教育部重点实验室，哈尔滨　150040；
2.林下经济资源研发与利用协同创新中心，哈尔滨　150040

出版日期:2015-07-20 发布日期:2016-03-09
基金资助:

Optimization of Ultrasonic-assisted Extraction of Total Flavonoids from Crataegus pinnatifida Leaves and Its Antioxidant Activities

LUO Meng^1,2；SONG Zhuo-Yue^1,2；HU Jiao-Yang^1,2；MU Fan-Song^1,2*；QIAO Qi^1,2；ZU Yuan-Gang^1,2

1.Key Laboratory of Forest Plant Ecology,Ministry of Education,Northeast Forestry University,Harbin　150040；
2.The Development and Utilization Collaborative Innovation Center of Forest Economic Resources,Harbin　150040

Online:2015-07-20 Published:2016-03-09
Supported by:

摘要/Abstract

摘要： 采用超声辅助提取法从山里红叶中提取总黄酮。通过BoxBehnken实验设计(BBD)结合响应面法(RSM)来优化超声提取的条件。影响山里红叶总黄酮提取效率的4个主要变量为液固比，温度，乙醇浓度和时间，得到的最佳值分别为15，40℃，40%，32 min。在此条件下，总黄酮的产率为15.50 mg·g^-1。体外抗氧化实验表明，山里红叶提取物的DPPH自由基清除能力为0.69 mg·mL^-1(以IC₅₀值表示) ，与传统的浸渍提取和热回流提取方法相比，超声提取的方法具有更好的抗氧化活性。实验结果表明超声提取法适用于提取山里红叶中的总黄酮，并且其提取物具有较好的抗氧化活性。

关键词: 山里红叶, 超声提取, 总黄酮, Box-Behnken设计, 抗氧化活性

Abstract: We used ultrasonic-assisted extraction(UAE) to extract the total flavonoids from Crataegus pinnatifida leaves, and used Box-Behnken design(BBD) combined with response surface methodology(RSM) to optimize extraction conditions. We determined the effects of four variables in terms of ratio of liquid/solid, temperature, ethanol concentration and time on the total flavonoids contents(TFC). The optimized extraction parameters obtained the highest total flavonoids yield were under the ratio of liquid/solid of 15, 40℃, and 40% of ethanol for 32 min. Under these conditions, the yield was 15.50 mg·g^-1. By in vitro antioxidant assays, the extract exhibited a strong DPPH radical-scavenging in ability with IC₅₀ value of 0.69 mg·mL^-1. Compared with conventional maceration extraction(ME) and heat-refluxing extraction(HE) methods, UAE method showed remarkable advantages in terms of better antioxidant activities. Overall, the present results indicated that UAE was a suitable method for the extraction of total flavonoids from C.pinnatifida leaves and its extracts were potential antioxidant ingredients.

Key words: Crataegus pinnatifida leaves, ultrasonic-assisted extraction, total flavonoids, Box-Behnken design, antioxidant activity

中图分类号:

罗猛1,2；宋卓悦1,2；胡娇阳1,2；牟璠松1,2*；乔琪1,2；祖元刚1,2. 超声法提取山里红叶总黄酮及其抗氧化活性研究[J]. 植物研究, 2015, 35(4): 632-637.

LUO Meng1,2；SONG Zhuo-Yue1,2；HU Jiao-Yang1,2；MU Fan-Song1,2*；QIAO Qi1,2；ZU Yuan-Gang1,2. Optimization of Ultrasonic-assisted Extraction of Total Flavonoids from Crataegus pinnatifida Leaves and Its Antioxidant Activities[J]. Bulletin of Botanical Research, 2015, 35(4): 632-637.

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超声法提取山里红叶总黄酮及其抗氧化活性研究

Optimization of Ultrasonic-assisted Extraction of Total Flavonoids from Crataegus pinnatifida Leaves and Its Antioxidant Activities

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