海南地区番木瓜花叶病病原的分子鉴定与多样性分析

doi:10.7525/j.issn.1673-5102.2014.05.017

植物研究 ›› 2014, Vol. 34 ›› Issue (5): 694-699.doi: 10.7525/j.issn.1673-5102.2014.05.017

海南地区番木瓜花叶病病原的分子鉴定与多样性分析

张雨良¹；黄启星¹；朱丽娣^1,2；孔华¹；曾会才¹；姜浩元^1,2；刘志昕^1*

1.中国热带农业科学院热带生物技术研究所，农业部热带作物生物学与遗传资源利用重点实验室，海口　571101；2.海南医学院理学院，海口　571101

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2014-09-20 发布日期:2015-03-19
通讯作者: 刘志昕
基金资助:

Molecular Identification and Diversity Analysis of Papaya Mosaic Viruses in Hainan Province

ZHANG Yu-Liang;HUANG Qi-Xing;ZHU Li-Di;KONG Hua;ZENG Hui-Cai;JIANG Hao-Yuan;LIU Zhi-Xin*

1.Key Laboratory of Biology and Genetic Resources of Tropical Crops,Ministry of Agriculture,Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Science,Haikou　571101；2.Department of Biochemistry,Hainan Medical College,Haikou　571101

Received:1900-01-01 Revised:1900-01-01 Online:2014-09-20 Published:2015-03-19
Contact: LIU Zhi-Xin
Supported by:

摘要/Abstract

摘要： 番木瓜环斑病毒(Papaya ring spot virus，PRSV)和番木瓜畸形花叶病毒（Papaya leaf-distortion mosaic virus，PLDMV）是热带、亚热带地区严重威胁番木瓜农业生产的主要病害。本研究对27份疑似番木瓜花叶病病叶样品进行分子鉴定，提取病叶总RNA，反转录获得对应的cDNA，根据PRSV病毒的CP、P1、Hc-Pro和NIb基因序列和PLDMV病毒的CP基因序列设计特异性引物进行PCR检测和测序分析。研究发现PRSV感染20例（74.1%），PLDMV感染3例（11.1%），PRSV和PLDMV交叉感染的样品1例（3.7%）。多样性分析结果表明，海南地区PRSV病毒存在3个株系，株系之间出现明显分化；海南地区PLDMV病毒与日本和中国台湾地区报道的PLDMV病毒株系有共同的起源。研究结果表明PRSV和PLDMV是海南地区番木瓜花叶病的主要病原，其中PRSV病毒3个株系之间P1基因同源性在95%以下，这可为后续研究构建抗PRSV和PLDMV的双价表达载体提供数据支持。

关键词: 番木瓜, PRSV, PLDMV

Abstract: Papaya ring spot virus(PRSV) and Papaya leaf-distortion mosaic virus(PLDMV) are dominant viruses causing papaya leaf mosaic disease in tropical and subtropical regions, threatening papaya planting industry. In this study, 27 suspected papaya leaf mosaic disease samples were collected and identified from Sanya, Ledong, Wanning and Qionghai in Hainan province. Total RNA from leaves was extracted and used for reverse transcription to obtain the corresponding cDNAs. Gene-specific primers were designed according to CP, P1, Hc-Pro and NIb gene nucleotide sequences from PRSV genome and CP gene nucleotide sequences from PLDMV genome. By PCR and sequencing, 20 PRSV-infected samples(74.1%), 3 PLDMV-infected samples(11.1%) and 1 PRSV-PLDMV cross-infected sample(3.7%) were identified. Phylogeny analysis showed that 3 PRSV varieties were existed in Hainan province with high strain differentiation, whereas, PLDMV varieties in Hainan province had a common origin with PLDMV varieties from Taiwan and Chian and Japan. The results showed that PRSV and PLDMV were dominant papaya mosaic viruses in Hainan, and the homology was below 95% during 3 PRSV varieties. This study would contribute to the subsequent construction of bivalent expression vector that could resist both PRSV and PLDMV viruses.

Key words: Papaya, PRSV, PLDMV

中图分类号:

S432.4+1

张雨良;黄启星;朱丽娣;孔华;曾会才;姜浩元;刘志昕*. 海南地区番木瓜花叶病病原的分子鉴定与多样性分析[J]. 植物研究, 2014, 34(5): 694-699.

ZHANG Yu-Liang;HUANG Qi-Xing;ZHU Li-Di;KONG Hua;ZENG Hui-Cai;JIANG Hao-Yuan;LIU Zhi-Xin*. Molecular Identification and Diversity Analysis of Papaya Mosaic Viruses in Hainan Province[J]. Bulletin of Botanical Research, 2014, 34(5): 694-699.

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海南地区番木瓜花叶病病原的分子鉴定与多样性分析

Molecular Identification and Diversity Analysis of Papaya Mosaic Viruses in Hainan Province

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