欢迎访问《植物研究》杂志官方网站,今天是 分享到:

植物研究 ›› 2014, Vol. 34 ›› Issue (3): 328-332.doi: 10.7525/j.issn.1673-5102.2014.03.007

• 论文 • 上一篇    下一篇

大叶蚁塔组织培养技术

陈锁良1;吕秀立2;崔心红2*;王铖2;张群2;朱义2;陆亮2   

  1. 1.江苏生太环境建设集团有限公司,丹阳 212300;2.上海市园林科学研究所,上海 200232
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2014-05-20 发布日期:2014-05-20
  • 通讯作者: 崔心红
  • 基金资助:
     

Tissue Culture of Gunnera manlcata L.

CHEN Suo-Liang;LV Xiu-Li;CUI Xin-Hong*;WANG Cheng;ZHANG Qun;ZHU Yi;LU Liang   

  1. 1.Jiangsu Shengtai Environmental Construction Group Co.,Ltd.,Danyang 212300;2.Shanghai Landscape Gardening Research Institute,Shanghai 200232
  • Received:1900-01-01 Revised:1900-01-01 Online:2014-05-20 Published:2014-05-20
  • Contact: CUI Xin-Hong
  • Supported by:
     

摘要: 以大叶蚁塔茎尖作为外植体诱导出无菌苗,再以无菌苗的嫩叶诱导不定芽发生,研究了其离体培养和不定芽再生的过程,成功建立了低成本的快速繁殖技术体系。结果表明:(1)无菌苗增殖培养基为MS+BA 1.0 mg·L-1+NAA 0.1 mg·L-1,培养30 d,增殖率稳定为3.80;(2)叶片可直接诱导再生出不定芽,其最佳培养基为MS+ZT 1.0 mg·L-1+NAA 0.1 mg·L-1,诱导率达95%,分化幼苗众多;(3)生根培养基为1/2MS+NAA 1 mg·L-1+蔗糖20 g·L-1时,生根率达95%。

关键词: 大叶蚁塔, 离体培养, 快速繁殖

Abstract: Using the apical buds of Gunnera manlcata as explants to induce the aseptic seedlings and then using the new leaves of aseptic seedling to induce the adventitious buds, in vitro culture and regeneration process of apical buds of G.manlcata were studied in this paper, and the quick propagation system in low cost was successfully established. The result showed: (1)Multiplication stage: MS+BA 1.0 mg·L-1+NAA 0.1 mg·L-1, cultured for 30 d, the stable multiplication rate was 3.80; (2)Induction stage: MS+ZT 1.0 mg·L-1+NAA 0.1 mg·L-1, which was proved to be superior to the others to be used to induce apical buds directly from the leaf explants, the induction rate reached 95%; (3)Rooting stage: 1/2MS+NAA 1 mg·L-1+sugar 20 g·L-1, the rooting rate reached 95%.

Key words: Gunnera manlcata L., tissue culture in vitro, rapid propagation

中图分类号: