欢迎访问《植物研究》杂志官方网站,今天是 分享到:

植物研究 ›› 2013, Vol. 33 ›› Issue (5): 599-604.doi: 10.7525/j.issn.1673-5102.2013.05.017

• 论文 • 上一篇    下一篇

ptc-miR801人工microRNA表达载体构建及功能初步研究

杨惠琴1,2;蒋晶1;刘明英1;乔桂荣1;姜彦成2;卓仁英1*   

  1. 1.中国林业科学研究院亚热带林业研究所,富阳 311400;2.新疆大学生命科学与技术学院,乌鲁木齐 830046
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2013-09-20 发布日期:2013-09-20
  • 通讯作者: 卓仁英
  • 基金资助:
     

Construction of Expression Vector of ptc-miR801 Gene and Function Analysis

YANG Hui-Qin;JIANG Jing;LIU Ming-Ying;QIAO Gui-Rong;JIANG Yan-Cheng;ZHUO Ren-Ying*   

  1. 1.Research Institute of Subtropical Forestry,Chinese Academy of Forestry,Fuyang 311400;2.College of Life Science and Technology,Xinjiang University,Urumqi 830046
  • Received:1900-01-01 Revised:1900-01-01 Online:2013-09-20 Published:2013-09-20
  • Contact: ZHUO Ren-Ying
  • Supported by:
     

摘要: 在构建盐胁迫下青杨microRNA文库中发现了ptc-miR801,为探索植物在盐胁迫条件下ptc-miR801参与胁迫应答的机制,本实验构建了植物表达载体pCAM2300-ami801,经根癌农杆菌EHA105介导、花序侵染法获得拟南芥转基因植株。RT-PCR半定量结果显示ptc-miR801可以在转基因拟南芥中超表达且NaCl胁迫下ptc-miRNA801转基因植株种子萌发率和根长显著高于野生型,说明ptc-miR801超表达增强了转基因拟南芥耐盐性。该试验为进一步研究miR801在杨树胁迫应答机制中的作用奠定基础。

关键词: miRNA, 表达载体, 拟南芥, 耐盐

Abstract: ptc-miR801 was identified from P.cathayana by microRNA library sequence, and it had been confirmed that ptc-miR801 was induced by salt stress. The putative targets were NAC-domain protein and Scarecrow-like gene, which were shown to play important roles in response to salt stress. To investigate the function of ptc-miR801, the artificial miR801 plant expression vector was constructed and transformed into Arabidopsis thaliana. Transformed plants were confirmed by PCR and RT-PCR. The results showed that amiRNA transcript level was markedly improved in transgenic plant compared with non-transgenic plants and the overexpression of ptc-miR801 could enhance the salt tolerance of transgenic plants. Our results would help to clarify the molecular mechanism of P.cathayana under salt stress.

Key words: miRNA, expression vector, Arabidopsis thaliana, salt stress

中图分类号: